Cysteine Dioxygenase Type 1 (CDO1) (NM_001801) Human Tagged ORF Clone

CAT#: RG206067

CDO1 (GFP-tagged) - Human cysteine dioxygenase, type I (CDO1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDO1
• Synonyms: CDO-I
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206067 representing NM_001801
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAGACCGAAGTGCTGAAGCCACGGACCCTGGCTGATCTGATCCGCATCCTGCACCAGCTCTTTG
  CCGGCGATGAGGTCAATGTAGAGGAGGTGCAGGCCATCATGGAAGCCTACGAGAGCGACCCCACCGAGTG
  GGCAATGTACGCCAAGTTCGACCAGTACAGGTATACCCGAAATCTTGTGGATCAAGGAAATGGAAAATTT
  AATCTGATGATTCTCTGTTGGGGTGAAGGACATGGCAGCAGTATTCATGATCATACCAACTCCCACTGCT
  TTCTGAAGATGCTACAGGGAAATCTAAAGGAGACATTATTTGCCTGGCCTGACAAAAAATCCAATGAGAT
  GGTCAAGAAGTCTGAAAGAGTCTTGAGGGAAAACCAGTGTGCCTACATCAATGATTCCGTTGGCTTACAT
  CGAGTAGAGAACATCAGCCATACGGAACCTGCTGTGAGCCTTCACTTGTACAGTCCACCTTTTGATACAT
  GCCATGCCTTTGATCAAAGAACAGGACATAAAAACAAAGTCACAATGACATTCCATAGTAAATTTGGAAT
  CAGAACTCCAAATGCAACTTCGGGCTCGCTGGAGAACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206067 representing NM_001801
  Red=Cloning site Green=Tags(s)
  
  MEQTEVLKPRTLADLIRILHQLFAGDEVNVEEVQAIMEAYESDPTEWAMYAKFDQYRYTRNLVDQGNGKF
  NLMILCWGEGHGSSIHDHTNSHCFLKMLQGNLKETLFAWPDKKSNEMVKKSERVLRENQCAYINDSVGLH
  RVENISHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMTFHSKFGIRTPNATSGSLENN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001801
• ORF Size: 600 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001801.2, NP_001792.2
• RefSeq Size: 1627 bp
• RefSeq ORF: 603 bp
• Locus ID: 1036
• Cytogenetics: 5q22.3
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Taurine and hypotaurine metabolism
• Gene Summary: ''