MFSD8 (NM_152778) Human Tagged ORF Clone

CAT#: RG206118

MFSD8 (GFP-tagged) - Human major facilitator superfamily domain containing 8 (MFSD8)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MFSD8
• Synonyms: CCMD; CLN7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206118 representing NM_152778
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCCTGCGGAACGAAAGTGAACAGGAGCCGCTCTTAGGCGACACACCTGGAAGCAGAGAATGGG
  ACATTTTAGAGACTGAAGAGCATTATAAGAGCCGATGGAGATCTATTAGGATTTTATATCTTACTATGTT
  TCTCAGCAGTGTAGGGTTTTCTGTAGTGATGATGTCCATATGGCCATATCTCCAAAAGATTGATCCGACA
  GCTGATACAAGTTTTTTGGGCTGGGTTATTGCTTCATATAGTCTTGGCCAAATGGTAGCTTCACCTATAT
  TTGGTTTATGGTCTAATTATAGACCAAGAAAAGAGCCTCTTATTGTCTCCATCTTGATTTCCGTGGCAGC
  CAACTGCCTCTATGCATATCTCCACATCCCAGCTTCTCATAATAAATACTACATGCTGGTTGCTCGTGGA
  TTGTTGGGAATTGGAGCAGGAAATGTAGCAGTTGTTAGATCATATACTGCTGGTGCTACTTCCCTTCAGG
  AAAGAACAAGTTCCATGGCAAACATAAGCATGTGTCAAGCATTAGGTTTTATTCTAGGTCCAGTTTTTCA
  GACTTGTTTTACATTCCTTGGAGAAAAAGGTGTGACATGGGATGTGATTAAACTGCAGATAAACATGTAT
  ACAACACCAGTTTTACTTAGCGCCTTCCTGGGAATTTTAAATATTATTCTGATCCTTGCCATACTAAGAG
  AACATCGTGTGGATGACTCAGGAAGACAGTGTAAAAGTATTAATTTTGAAGAAGCAAGTACAGATGAAGC
  TCAGGTTCCCCAAGGAAATATTGACCAGGTTGCTGTTGTGGCCATCAATGTTCTGTTTTTTGTGACTCTA
  TTTATCTTTGCCCTTTTTGAAACCATCATTACTCCATTAACAATGGATATGTATGCCTGGACTCAAGAAC
  AAGCTGTGTTATATAATGGCATAATACTTGCTGCTCTTGGGGTTGAAGCCGTTGTTATTTTCTTAGGAGT
  TAAGTTGCTTTCCAAAAAGATTGGCGAGCGTGCTATTCTACTGGGAGGACTCATCGTTGTATGGGTTGGC
  TTCTTTATCTTGTTACCTTGGGGAAATCAATTTCCCAAAATACAGTGGGAAGATTTGCACAATAATTCAA
  TCCCTAATACCACATTTGGGGAAATTATTATTGGTCTTTGGAAGTCTCCAATGGAAGATGACAATGAAAG
  ACCAACTGGTTGCTCGATTGAACAAGCCTGGTGCCTCTACACCCCGGTGATTCATCTGGCCCAGTTCCTT
  ACATCAGCTGTGCTAATAGGATTAGGCTATCCAGTCTGCAATCTTATGTCCTATACTCTATATTCAAAAA
  TTCTAGGACCAAAACCTCAGGGTGTATACATGGGCTGGTTAACAGCATCTGGAAGTGGAGCCCGGATTCT
  TGGGCCTATGTTCATCAGCCAAGTGTATGCTCACTGGGGACCACGATGGGCATTCAGCCTGGTGTGTGGA
  ATAATAGTGCTCACCATCACCCTCCTGGGAGTGGTTTACAAAAGACTCATTGCTCTTTCTGTAAGATATG
  GGAGGATTCAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206118 representing NM_152778
  Red=Cloning site Green=Tags(s)
  
  MAGLRNESEQEPLLGDTPGSREWDILETEEHYKSRWRSIRILYLTMFLSSVGFSVVMMSIWPYLQKIDPT
  ADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLHIPASHNKYYMLVARG
  LLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPVFQTCFTFLGEKGVTWDVIKLQINMY
  TTPVLLSAFLGILNIILILAILREHRVDDSGRQCKSINFEEASTDEAQVPQGNIDQVAVVAINVLFFVTL
  FIFALFETIITPLTMDMYAWTQEQAVLYNGIILAALGVEAVVIFLGVKLLSKKIGERAILLGGLIVVWVG
  FFILLPWGNQFPKIQWEDLHNNSIPNTTFGEIIIGLWKSPMEDDNERPTGCSIEQAWCLYTPVIHLAQFL
  TSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHWGPRWAFSLVCG
  IIVLTITLLGVVYKRLIALSVRYGRIQE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152778
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152778.1, NP_689991.1
• RefSeq Size: 1909
• RefSeq ORF: 1557
• Locus ID: 256471
• Domains: sugar_tr
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: This gene encodes a ubiquitous integral membrane protein that contains a transporter domain and a major facilitator superfamily (MFS) domain. Other members of the major facilitator superfamily transport small solutes through chemiosmotic ion gradients. The substrate transported by this protein is unknown. The protein likely localizes to lysosomal membranes. Mutations in this gene are correlated with a variant form of late infantile-onset neuronal ceroid lipofuscinoses (vLINCL). [provided by RefSeq, Oct 2008]