Probable hydrolase PNKD (PNKD) (NM_015488) Human Tagged ORF Clone

CAT#: RG206179

PNKD (GFP-tagged) - Human paroxysmal nonkinesigenic dyskinesia (PNKD), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PNKD
• Synonyms: BRP17; DYT8; FKSG19; FPD1; KIPP1184; MR-1; MR-1S; MR1; PDC; PKND1; PNKD1; TAHCCP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206179 representing NM_015488
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGTGGTAGCTGCTACGGCGCTGAAGAGCCGGGGGGCGAGAAATGCCCGCGTCCTCCGGGGGA
  TTCTCGCAGGAGCCACAGCTAACAAGGTTTCTCATAACAGGACCCGGGCCCTGCAAAGCCACAGCTCCTC
  AGAGGGCAAGGAGGAACCTGAACCCCTATCCCCGGAGCTGGAATACATTCCCAGAAAGAGGGGCAAGAAC
  CCCATGAAAGCTGTGGGACTGGCCTGGTACAGCCTGTACACCCGCACCTGGCTCGGGTACCTCTTCTACC
  GACAGCAGCTGCGCAGGGCTCGGAATCGCTACCCTAAAGGCCACTCGAAAACCCAGCCCCGCCTCTTCAA
  TGGAGTGAAGGTGCTTCCCATCCCTGTCCTCTCGGACAACTACAGCTACCTCATCATCGACACCCAGGCC
  CAGCTGGCTGTGGCTGTGGACCCTTCAGACCCTCGGGCTGTGCAGGCTTCCATTGAAAAGGAAGGGGTCA
  CCTTGGTCGCCATTCTGTGTACTCACAAGCACTGGGACCACAGTGGAGGGAACCGTGACCTCAGCCGGCG
  GCACCGGGACTGTCGGGTGTACGGGAGCCCTCAGGACGGCATCCCCTACCTCACCCATCCCCTGTGTCAT
  CAAGATGTGGTCAGCGTGGGACGGCTTCAGATCCGGGCCCTGGCTACACCTGGCCACACACAAGGCCATC
  TGGTCTACCTACTGGATGGGGAGCCCTACAAGGGTCCCTCCTGCCTCTTCTCAGGGGACCTGCTCTTCCT
  CTCTGGCTGTGGGCGGACCTTTGAGGGCAATGCAGAGACCATGCTGAGCTCACTGGACACTGTGCTGGGG
  CTAGGGGATGACACCCTTCTGTGGCCTGGTCATGAGTATGCAGAGGAGAACCTGGGCTTTGCAGGTGTGG
  TGGAGCCCGAGAACCTGGCCCGGGAGAGGAAGATGCAGTGGGTGCAGCGGCAGCGGCTGGAGCGCAAGGG
  CACGTGCCCATCTACCCTGGGAGAGGAGCGCTCCTACAACCCGTTCCTGAGAACCCACTGCCTGGCGCTA
  CAGGAGGCTCTGGGGCCGGGGCCGGGCCCCACTGGGGATGATGACTACTCCCGGGCCCAGCTCCTGGAAG
  AGCTCCGCCGGCTGAAGGATATGCACAAGAGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206179 representing NM_015488
  Red=Cloning site Green=Tags(s)
  
  MAAVVAATALKSRGARNARVLRGILAGATANKVSHNRTRALQSHSSSEGKEEPEPLSPELEYIPRKRGKN
  PMKAVGLAWYSLYTRTWLGYLFYRQQLRRARNRYPKGHSKTQPRLFNGVKVLPIPVLSDNYSYLIIDTQA
  QLAVAVDPSDPRAVQASIEKEGVTLVAILCTHKHWDHSGGNRDLSRRHRDCRVYGSPQDGIPYLTHPLCH
  QDVVSVGRLQIRALATPGHTQGHLVYLLDGEPYKGPSCLFSGDLLFLSGCGRTFEGNAETMLSSLDTVLG
  LGDDTLLWPGHEYAEENLGFAGVVEPENLARERKMQWVQRQRLERKGTCPSTLGEERSYNPFLRTHCLAL
  QEALGPGPGPTGDDDYSRAQLLEELRRLKDMHKSK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015488
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015488.4, NP_056303.3
• RefSeq Size: 3129
• RefSeq ORF: 1158
• Locus ID: 25953
• Protein Families: Transmembrane
• Gene Summary: This gene is thought to play a role in the regulation of myofibrillogenesis. Mutations in this gene have been associated with the movement disorder paroxysmal non-kinesigenic dyskinesia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]