FBXW8 (NM_153348) Human Tagged ORF Clone

CAT#: RG206199

FBXW8 (GFP-tagged) - Human F-box and WD repeat domain containing 8 (FBXW8), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FBXW8
• Synonyms: FBW6; FBW8; FBX29; FBXO29; FBXW6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206199 representing NM_153348
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACTACAGCCTGGATGAGTTCCGTCGGCGCTGGCAGGAGGAGCTGGCGCAGGCCCAGGCGCCGA
  AGAAGCGGCGACGGCCCGAGGCTGCCGAGAGGCGGGCTCGGCGGCCGGAGGTGGGCTCCGGGCGCAGCGA
  ACAGGCCTCGGGGGACCCGGCGCTGGCCCAGGGTCTCCTGGAGGGCGCGGGGAGGCCCCCGGCGGCGCGG
  GCGACTCGGGCCGAGGGGCAGGACGTAGCGAGCCGCTCACGTTCTCCTCTGGCCCGCGAGGGCGCCGGGG
  GCGGGGAGCAGCTGGTGGACCAGCTCATCCGCGACCTGAATGAAATGAATGATGTGCCTTTCTTTGATAT
  CCAACTGCCTTACGAATTGGCAATCAATATATTTCAGTATCTGGACAGGAAAGAACTAGGAAGATGTGCA
  CAGGTGAGCAAGACGTGGAAGGTGATTGCAGAGGATGAGGTGCTGTGGTACAGGCTGTGCCAGCAGGAAG
  GGCACCTTCCGGATAGCAGCATCTCTGACTATTCTTGCTGGAAGCTCATCTTCCAAGAGTGCCGAGCCAA
  GGAACACATGTTACAAACCAACTGGAAGAATCGCAAAGGTGCCGTGAGCGAGCTGGAGCATGTTCCTGAC
  ACAGTTTTGTGTGATGTGCATTCTCACGATGGTGTGGTCATTGCGGGATATACATCAGGGGATGTGAGAG
  TGTGGGACACCCGCACCTGGGACTACGTAGCCCCCTTCCTGGAATCAGAGGACGAGGAGGATGAGCCTGG
  AATGCAGCCAAATGTCTCCTTTGTGAGGATAAACAGCTCGTTGGCAGTAGCAGCTTATGAGGATGGGTTT
  CTTAATATTTGGGATTTAAGGACCGGAAAGTACCCTGTTCATCGTTTTGAGCACGATGCAAGAATACAGG
  CACTAGCCCTCAGCCAGGACGATGCAACCGTGGCCACAGCTTCTGCTTTTGATGTCGTGATGTTATCCCC
  CAATGAGGAGGGGTACTGGCAGATAGCTGCGGAATTTGAAGTTCCGAAACTGGTTCAGTACCTTGAAATA
  GTTCCAGAAACCAGAAGGTACCCTGTGGCAGTAGCCGCTGCTGGAGATCTGATGTACCTGCTCAAAGCCG
  AAGACTCCGCCAGAACCATCCTTTACGCCCACGGCCCGCCTGTCACATGTCTAGACGTCTCGGCCAACCA
  AGTTGCTTTTGGTGTACAGGGTCTGGGATGGGTGTACGAAGGAAGCAAGATCCTGGTGTATAGCCTGGAA
  GCAGGACGCCGCCTCTTGAAGCTGGGTAACGTTCTCCGTGACTTCACGTGTGTCAACCTCAGCGACAGCC
  CTCCCAACCTCATGGTCAGTGGCAACATGGACGGGAGGGTGAGGATCCACGACCTCCGCAGTGGTAACAT
  CGCCCTGTCGCTCTCCGCCCATCAGCTCAGGGTCTCTGCTGTGCAGATGGATGACTGGAAGATCGTCAGT
  GGAGGCGAGGAAGGCCTGGTGTCCGTGTGGGATTATCGGATGAACCAGAAGCTGTGGGAGGTGTATTCCG
  GGCACCCGGTGCAGCACATCTCATTCAGCAGCCACAGCCTCATCACGGCCAACGTGCCTTACCAGACGGT
  AATGCGAAACGCCGACCTGGACAGCTTCACTACTCACAGGAGACACCGGGGGCTGATCCGCGCCTACGAG
  TTTGCGGTGGACCAGCTGGCCTTCCAGAGCCCTCTCCCTGTCTGCCGTTCATCCTGTGACGCCATGGCCA
  CTCACTACTACGACCTCGCACTGGCCTTTCCCTATAACCATGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206199 representing NM_153348
  Red=Cloning site Green=Tags(s)
  
  MDDYSLDEFRRRWQEELAQAQAPKKRRRPEAAERRARRPEVGSGRSEQASGDPALAQGLLEGAGRPPAAR
  ATRAEGQDVASRSRSPLAREGAGGGEQLVDQLIRDLNEMNDVPFFDIQLPYELAINIFQYLDRKELGRCA
  QVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISDYSCWKLIFQECRAKEHMLQTNWKNRKGAVSELEHVPD
  TVLCDVHSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESEDEEDEPGMQPNVSFVRINSSLAVAAYEDGF
  LNIWDLRTGKYPVHRFEHDARIQALALSQDDATVATASAFDVVMLSPNEEGYWQIAAEFEVPKLVQYLEI
  VPETRRYPVAVAAAGDLMYLLKAEDSARTILYAHGPPVTCLDVSANQVAFGVQGLGWVYEGSKILVYSLE
  AGRRLLKLGNVLRDFTCVNLSDSPPNLMVSGNMDGRVRIHDLRSGNIALSLSAHQLRVSAVQMDDWKIVS
  GGEEGLVSVWDYRMNQKLWEVYSGHPVQHISFSSHSLITANVPYQTVMRNADLDSFTTHRRHRGLIRAYE
  FAVDQLAFQSPLPVCRSSCDAMATHYYDLALAFPYNHV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153348
• ORF Size: 1794 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153348.2, NP_699179.2
• RefSeq Size: 4871
• RefSeq ORF: 1797
• Locus ID: 26259
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]