FOXI1 (NM_144769) Human Tagged ORF Clone

CAT#: RG206430

FOXI1 (GFP-tagged) - Human forkhead box I1 (FOXI1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FOXI1
• Synonyms: FKH10; FKHL10; FREAC-6; FREAC6; HFH-3; HFH3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206430 representing NM_144769
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCTTCGACCTGCCGGCGCCCTCCCCACCTCGCTGCAGCCCCCAGTTCCCCAGCATCGGCCAGG
  AGCCCCCCGAGATGAACCTCTACTATGAGAACTTCTTCCACCCACAGGGCGTGCCCAGCCCTCAGCGGCC
  CTCCTTCGAGGGGGGCGGCGAGTATGGGGCCACCCCCAACCCCTACCTCTGGTTCAACGGGCCCACCATG
  ACCCCGCCACCCTACCTGCCCGGCCCCAACGCCAGCCCCTTCCTGCCCCAGGCCTATGGAGTGCAGAGGC
  CGCTGCTGCCCAGCGTGTCGGGGCTTGGGGGGAGCGACCTGGGCTGGCTGCCCATCCCCTCGCAGGAGGA
  GCTGATGAAGCTGGTGCGGCCACCCTATTCCTACTCGGCTCTCATCGCCATGGCCATCCACGGGGCACCC
  GACAAGCGCCTCACTCTCAGCCAGATCTACCAGTACGTGGCCGACAACTTCCCCTTCTACAACAAGAGCA
  AGGCCGGCTGGCAGAACTCCATCCGCCACAACCTGTCGCTCAACGACTGCTTCAAGAAGGTGCCCCGCGA
  CGAGGACGACCCGGCCTATGTGAGCGGGGGGAGCCCCACGAGCCACCCCTTGGTCACACCAGGACTGAGC
  CCTGAGCCCAGTGACAAGACGGGGCAGAACTCACTGACCTTCAACTCCTTCTCCCCGCTCACCAACCTCA
  GCAACCACAGCGGTGGGGGTGACTGGGCGAACCCCATGCCCACCAACATGCTCAGCTACGGAGGATCTGT
  GCTCAGCCAATTCAGCCCTCACTTCTACAACAGTGTCAACACCAGTGGTGTCCTCTACCCCAGGGAGGGC
  ACCGAGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206430 representing NM_144769
  Red=Cloning site Green=Tags(s)
  
  MSSFDLPAPSPPRCSPQFPSIGQEPPEMNLYYENFFHPQGVPSPQRPSFEGGGEYGATPNPYLWFNGPTM
  TPPPYLPGPNASPFLPQAYGVQRPLLPSVSGLGGSDLGWLPIPSQEELMKLVRPPYSYSALIAMAIHGAP
  DKRLTLSQIYQYVADNFPFYNKSKAGWQNSIRHNLSLNDCFKKVPRDEDDPAYVSGGSPTSHPLVTPGLS
  PEPSDKTGQNSLTFNSFSPLTNLSNHSGGGDWANPMPTNMLSYGGSVLSQFSPHFYNSVNTSGVLYPREG
  TEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144769
• ORF Size: 849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144769.2, NP_658982.1
• RefSeq Size: 2011 bp
• RefSeq ORF: 852 bp
• Locus ID: 2299
• Cytogenetics: 5q35.1
• Protein Families: Transcription Factors
• Gene Summary: 'This gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene may play an important role in the development of the cochlea and vestibulum, as well as in embryogenesis. The encoded protein has been found to be required for the transcription of four subunits of a proton pump found in the inner ear, the kidney, and the epididymis. Mutations in this gene have been associated with deafness, autosomal recessive 4. [provided by RefSeq, Jan 2017]'