HCAR2 (NM_177551) Human Tagged ORF Clone

CAT#: RG206527

HCAR2 (GFP-tagged) - Human G protein-coupled receptor 109A (GPR109A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HCAR2
• Synonyms: GPR109A; HCA2; HM74a; HM74b; NIACR1; Puma-g; PUMAG
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206527 representing NM_177551
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCGGCACCATCTGCAGGATCACTTTCTGGAAATAGACAAGAAGAACTGCTGCGTGTTCCGAGATG
  ACTTCATTGTCAAGGTGTTGCCGCCGGTGTTGGGGCTGGAGTTTATCTTCGGGCTTCTGGGCAATGGCCT
  TGCCCTGTGGATTTTCTGTTTCCACCTCAAGTCCTGGAAATCCAGCCGGATTTTCCTGTTCAACCTGGCA
  GTGGCTGACTTTCTACTGATCATCTGCCTGCCCTTCCTGATGGACAACTATGTGAGGCGTTGGGACTGGA
  AGTTTGGGGACATCCCTTGCCGGCTGATGCTCTTCATGTTGGCTATGAACCGCCAGGGCAGCATCATCTT
  CCTCACGGTGGTGGCGGTAGACAGGTATTTCCGGGTGGTCCATCCCCACCACGCCCTGAACAAGATCTCC
  AATCGGACAGCAGCCATCATCTCTTGCCTTCTGTGGGGCATCACTATTGGCCTGACAGTCCACCTCCTGA
  AGAAGAAGATGCCGATCCAGAATGGCGGTGCAAATTTGTGCAGCAGCTTCAGCATCTGCCATACCTTCCA
  GTGGCACGAAGCCATGTTCCTCCTGGAGTTCTTCCTGCCCCTGGGCATCATCCTGTTCTGCTCAGCCAGA
  ATTATCTGGAGCCTGCGGCAGAGACAAATGGACCGGCATGCCAAGATCAAGAGAGCCATCACCTTCATCA
  TGGTGGTGGCCATCGTCTTTGTCATCTGCTTCCTTCCCAGCGTGGTTGTGCGGATCCGCATCTTCTGGCT
  CCTGCACACTTCGGGCACGCAGAATTGTGAAGTGTACCGCTCGGTGGACCTGGCGTTCTTTATCACTCTC
  AGCTTCACCTACATGAACAGCATGCTGGACCCCGTGGTGTACTACTTCTCCAGCCCATCCTTTCCCAACT
  TCTTCTCCACTTTGATCAACCGCTGCCTCCAGAGGAAGATGACAGGTGAGCCAGATAATAACCGCAGCAC
  GAGCGTCGAGCTCACAGGGGACCCCAACAAAACCAGAGGCGCTCCAGAGGCGTTAATGGCCAACTCCGGT
  GAGCCATGGAGCCCCTCTTATCTGGGCCCAACCTCTCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206527 representing NM_177551
  Red=Cloning site Green=Tags(s)
  
  MNRHHLQDHFLEIDKKNCCVFRDDFIVKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLA
  VADFLLIICLPFLMDNYVRRWDWKFGDIPCRLMLFMLAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKIS
  NRTAAIISCLLWGITIGLTVHLLKKKMPIQNGGANLCSSFSICHTFQWHEAMFLLEFFLPLGIILFCSAR
  IIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIRIFWLLHTSGTQNCEVYRSVDLAFFITL
  SFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKMTGEPDNNRSTSVELTGDPNKTRGAPEALMANSG
  EPWSPSYLGPTSP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177551
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177551.3, NP_808219.1
• RefSeq Size: 2082
• RefSeq ORF: 1092
• Locus ID: 338442
• Protein Families: Druggable Genome, GPCR, Transmembrane