GCAP1 (GUCA1A) (NM_000409) Human Tagged ORF Clone
CAT#: RG206595
- TrueORF®
GUCA1A (GFP-tagged) - Human guanylate cyclase activator 1A (retina) (GUCA1A)
"NM_000409" in other vectors (7)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | LOC118142757 |
Synonyms | GUCA1A; GUCA1ANB-GUCA1A |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG206595 representing NM_000409
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACGTGATGGAGGGAAAGTCAGTGGAGGAGCTGAGCAGCACCGAGTGCCACCAGTGGTACAAGA AGTTCATGACTGAGTGCCCCTCTGGCCAACTCACCCTCTATGAGTTCCGCCAGTTCTTCGGCCTCAAGAA CCTGAGCCCGTCGGCCAGCCAGTACGTGGAACAGATGTTTGAGACTTTTGACTTCAACAAGGACGGCTAC ATTGATTTCATGGAGTACGTGGCAGCGCTCAGCTTGGTCCTCAAGGGGAAGGTGGAACAGAAGCTCCGCT GGTACTTCAAGCTCTATGATGTAGATGGCAACGGCTGCATTGACCGCGATGAGCTGCTCACCATCATCCA GGCCATTCGCGCCATTAACCCCTGCAGCGATACCACCATGACTGCAGAGGAGTTCACCGATACAGTGTTC TCCAAGATTGACGTCAACGGGGATGGGGAACTCTCCCTGGAAGAGTTTATAGAGGGCGTCCAGAAGGACC AGATGCTCCTGGACACACTGACACGAAGCCTGGACCTTACCCGCATCGTGCGCAGGCTCCAGAATGGCGA GCAAGACGAGGAGGGGGCTGACGAGGCCGCTGAGGCAGCCGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206595 representing NM_000409
Red=Cloning site Green=Tags(s) MGNVMEGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSPSASQYVEQMFETFDFNKDGY IDFMEYVAALSLVLKGKVEQKLRWYFKLYDVDGNGCIDRDELLTIIQAIRAINPCSDTTMTAEEFTDTVF SKIDVNGDGELSLEEFIEGVQKDQMLLDTLTRSLDLTRIVRRLQNGEQDEEGADEAAEAAG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000409 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000409.2, NP_000400.2 |
RefSeq Size | 1976 bp |
RefSeq ORF | 606 bp |
Locus ID | 2978 |
Cytogenetics | 6p21.1 |
Domains | EFh |
Protein Families | Druggable Genome |
Protein Pathways | Olfactory transduction |
Gene Summary | 'This gene encodes an enzyme that plays a role in the recovery of retinal photoreceptors from photobleaching. This enzyme promotes the activity of retinal guanylyl cyclase-1 (GC1) at low calcium concentrations and inhibits GC1 at high calcium concentrations. Mutations in this gene can cause cone dystrophy 3 and code-rod dystrophy 14. provided by RefSeq, Jul 2020]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108438 | GUCA1A (untagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A) |
USD 310.00 |
|
SC324283 | GUCA1A (untagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A) |
USD 310.00 |
|
RC206595 | GUCA1A (Myc-DDK-tagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A) |
USD 98.00 |
|
RC206595L1 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), Myc-DDK-tagged |
USD 768.00 |
|
RC206595L2 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), mGFP tagged |
USD 620.00 |
|
RC206595L3 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), Myc-DDK-tagged |
USD 620.00 |
|
RC206595L4 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), mGFP tagged |
USD 620.00 |
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