PPM1K (NM_152542) Human Tagged ORF Clone

CAT#: RG207144

PPM1K (GFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1K (PPM1K), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPM1K
• Synonyms: BDP; MSUDMV; PP2Ckappa; PP2Cm; PTMP; UG0882E07
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207144 representing NM_152542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACAGCTGCCTTAATTACTTTGGTCAGAAGTGGTGGGAACCAGGTGAGAAGGAGAGTGCTGCTAA
  GCTCCCGCCTGCTGCAGGACGACAGGCGGGTGACACCCACGTGCCACAGCTCCACTTCAGAGCCTAGGTG
  TTCTCGGTTTGACCCAGATGGTAGTGGGAGTCCAGCTACCTGGGACAATTTTGGGATCTGGGATAACCGC
  ATTGATGAGCCAATTCTGCTGCCACCCAGCATTAAGTATGGCAAGCCAATTCCCAAAATCAGCTTGGAAA
  AGGTGGGGTGCGCCTCACAGATTGGCAAACGGAAAGAGAATGAAGATCGGTTTGACTTCGCTCAGCTGAC
  AGATGAGGTCCTGTACTTTGCAGTGTATGATGGACACGGTGGACCTGCAGCAGCTGATTTCTGTCATACC
  CACATGGAGAAATGTATTATGGATTTGCTTCCTAAGGAGAAGAACTTGGAAACTCTGTTGACCTTGGCTT
  TTCTAGAAATAGATAAAGCCTTTTCGAGTCATGCCCGCCTGTCTGCTGATGCAACTCTTCTGACCTCTGG
  GACTACTGCAACAGTAGCCCTATTGCGAGATGGTATTGAACTGGTTGTAGCCAGTGTTGGGGACAGCCGG
  GCTATTTTGTGTAGAAAAGGAAAACCCATGAAGCTGACCATTGACCATACTCCAGAAAGAAAAGATGAAA
  AAGAAAGGATCAAGAAATGTGGTGGTTTTGTAGCTTGGAATAGTTTGGGGCAGCCTCACGTAAATGGCAG
  GCTTGCAATGACAAGAAGTATTGGAGATTTGGACCTTAAGACCAGTGGTGTCATAGCAGAACCTGAAACT
  AAGAGGATTAAGTTACATCATGCTGATGACAGCTTCCTGGTCCTCACCACAGATGGAATTAACTTCATGG
  TGAATAGTCAAGAGATTTGTGACTTTGTCAATCAGTGCCATGATCCCAACGAAGCAGCCCATGCGGTGAC
  TGAACAGGCAATACAGTACGGTACTGAGGATAACAGTACTGCAGTAGTAGTGCCTTTTGGTGCCTGGGGA
  AAATATAAGAACTCTGAAATCAACTTCTCATTCAGCAGAAGCTTTGCCTCCAGTGGACGATGGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207144 representing NM_152542
  Red=Cloning site Green=Tags(s)
  
  MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDNR
  IDEPILLPPSIKYGKPIPKISLEKVGCASQIGKRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHT
  HMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSR
  AILCRKGKPMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIAEPET
  KRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPFGAWG
  KYKNSEINFSFSRSFASSGRWA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152542
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152542.2, NP_689755.2
• RefSeq Size: 2103
• RefSeq ORF: 1119
• Locus ID: 152926
• Domains: PP2C
• Protein Families: Phosphatase
• Gene Summary: This gene encodes a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. The encoded protein, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore. [provided by RefSeq, Sep 2012]