Keratocan (KERA) (NM_007035) Human Tagged ORF Clone

CAT#: RG207509

KERA (GFP-tagged) - Human keratocan (KERA)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KERA
• Synonyms: CNA2; KTN; SLRR2B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207509 representing NM_007035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCACAATCTGTTTCATCATGTGGGTGTTATTCATAACAGACACTGTGTGGTCTAGAAGTGTAA
  GGCAGGTCTATGAAGTACATGATTCAGATGATTGGACTATTCATGACTTCGAGTGTCCCATGGAATGTTT
  CTGCCCACCCAGTTTTCCTACTGCTTTATATTGTGAAAATAGAGGTCTCAAAGAAATTCCTGCTATTCCT
  TCAAGAATTTGGTATCTTTATCTTCAAAACAACCTGATAGAAACCATTCCTGAAAAGCCATTTGAGAATG
  CCACCCAGCTAAGATGGATAAATCTAAACAAGAACAAAATAACCAACTACGGAATTGAAAAAGGAGCCCT
  AAGCCAGCTGAAGAAGTTGCTCTTCTTATTTCTGGAAGATAATGAGCTAGAGGAGGTACCTTCTCCATTG
  CCAAGAAGTTTAGAACAATTACAATTAGCTAGAAATAAGGTGTCCAGAATTCCTCAAGGGACCTTTAGCA
  ATCTGGAGAACCTGACCCTTCTTGACCTACAGAACAACAAATTAGTGGACAATGCCTTTCAAAGAGACAC
  TTTTAAAGGACTCAAGAATCTCATGCAGCTAAACATGGCCAAGAATGCCCTGAGGAATATGCCTCCAAGA
  TTACCAGCCAATACAATGCAGTTGTTTTTAGACAACAATTCCATTGAAGGAATACCAGAAAATTATTTTA
  ATGTGATTCCTAAAGTGGCCTTTTTGAGACTAAATCACAACAAACTGTCAGATGAGGGTCTCCCATCAAG
  AGGATTTGATGTATCATCAATTCTAGATCTTCAACTGTCGCACAATCAACTCACAAAGGTTCCCCGAATC
  AGTGCTCATCTGCAGCACCTTCACCTTGATCATAACAAAATTAAAAGTGTGAATGTCTCTGTAATATGTC
  CCAGCCCATCCATGCTGCCTGCAGAACGAGATTCCTTCAGTTATGGACCTCATCTTCGCTACCTCCGTCT
  GGATGGAAATGAAATCAAACCACCAATTCCAATGGCTTTAATGACCTGCTTCAGACTTCTGCAGGCTGTC
  ATTATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207509 representing NM_007035
  Red=Cloning site Green=Tags(s)
  
  MAGTICFIMWVLFITDTVWSRSVRQVYEVHDSDDWTIHDFECPMECFCPPSFPTALYCENRGLKEIPAIP
  SRIWYLYLQNNLIETIPEKPFENATQLRWINLNKNKITNYGIEKGALSQLKKLLFLFLEDNELEEVPSPL
  PRSLEQLQLARNKVSRIPQGTFSNLENLTLLDLQNNKLVDNAFQRDTFKGLKNLMQLNMAKNALRNMPPR
  LPANTMQLFLDNNSIEGIPENYFNVIPKVAFLRLNHNKLSDEGLPSRGFDVSSILDLQLSHNQLTKVPRI
  SAHLQHLHLDHNKIKSVNVSVICPSPSMLPAERDSFSYGPHLRYLRLDGNEIKPPIPMALMTCFRLLQAV
  II
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007035
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007035.3, NP_008966.1
• RefSeq Size: 2543
• RefSeq ORF: 1059
• Locus ID: 11081
• Protein Families: Secreted Protein
• Gene Summary: The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2). [provided by RefSeq, May 2010]