DDAH1 (NM_012137) Human Tagged ORF Clone

CAT#: RG207548

DDAH1 (GFP-tagged) - Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDAH1
• Synonyms: DDAH; DDAH-1; DDAHI; HEL-S-16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207548 representing NM_012137
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGCTCGGCCACCCCGCCGCCTTCGGCCGGGCCACCCACGCCGTGGTGCGGGCGCTACCCGAGT
  CGCTCGGCCAGCACGCGCTGAGAAGCGCCAAGGGCGAGGAGGTGGACGTCGCCCGCGCGGAACGGCAGCA
  CCAGCTCTACGTGGGCGTGCTGGGCAGCAAGCTGGGGCTGCAGGTGGTGGAGCTGCCGGCCGACGAGAGC
  CTTCCGGACTGCGTCTTCGTGGAGGACGTGGCCGTGGTGTGCGAGGAGACGGCCCTCATCACCCGACCCG
  GGGCGCCGAGCCGGAGGAAGGAGGTTGACATGATGAAAGAAGCATTAGAAAAACTTCAGCTCAATATAGT
  AGAGATGAAAGATGAAAATGCAACTTTAGATGGCGGAGATGTTTTATTCACAGGCAGAGAATTTTTTGTG
  GGCCTTTCCAAAAGGACAAATCAACGAGGTGCTGAAATCTTGGCTGATACTTTTAAGGACTATGCAGTCT
  CCACAGTGCCAGTGGCAGATGGGTTGCATTTGAAGAGTTTCTGCAGCATGGCTGGGCCTAACCTGATCGC
  AATTGGGTCTAGTGAATCTGCACAGAAGGCCCTTAAGATCATGCAACAGATGAGTGACCACCGCTACGAC
  AAACTCACTGTGCCTGATGACATAGCAGCAAACTGTATATATCTAAATATCCCCAACAAAGGGCACGTCT
  TGCTGCACCGAACCCCGGAAGAGTATCCAGAAAGTGCAAAGGTTTATGAGAAACTGAAGGACCATATGCT
  GATCCCCGTGAGCATGTCTGAACTGGAAAAGGTGGATGGGCTGCTCACCTGCTGCTCAGTTTTAATTAAC
  AAGAAAGTAGACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207548 representing NM_012137
  Red=Cloning site Green=Tags(s)
  
  MAGLGHPAAFGRATHAVVRALPESLGQHALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADES
  LPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFV
  GLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYD
  KLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSVLIN
  KKVDS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012137
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012137.2, NP_036269.1
• RefSeq Size: 3949
• RefSeq ORF: 858
• Locus ID: 23576
• Domains: Amidinotransf
• Gene Summary: This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]