SARA (ZFYVE9) (NM_004799) Human Tagged ORF Clone

CAT#: RG207578

ZFYVE9 (GFP-tagged) - Human zinc finger, FYVE domain containing 9 (ZFYVE9), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZFYVE9
• Synonyms: MADHIP; NSP; PPP1R173; SARA; SMADIP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207578 representing NM_004799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATTACTTCCAAGCAGAAGCTTACAACCTGGACAAGGTGTTAGATGAATTTGAACAAAACGAAG
  ATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTAGATCCCCCTTCTCACCGGCT
  GTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCAGTTTCTAATGAGTCACAACCACAACTGAAA
  GTCTTCTCCCTGGCTCATTCAGCTCCCCTGACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACT
  GTAATCTAAATCCAGAGATTGCCACAATGTGGATTGATGAAAATGCTGTTGCAGAAGACCAGTTAATTAA
  GAGAAACTATAGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCT
  TGTCTGCCAGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGGACATTACAAA
  ACGATTTACAGGATTGTAATAATTATAATAGTCAATCCCTTATGGATGCTTTTAGCTGTTCACTGGATAA
  TGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAATGAGTCCACTGAAAAAGATATGAATTCAGAG
  AAACAAATGGATCCATTGAATAGACCGAAAACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCAT
  CTGATAGTCTAGCCAGTGTCTGTTCCCCTTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTC
  CATGTCTGCGATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGATGGATGTCCTGCT
  GTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCTAGGACAGATC
  TAGGGAGTCCAAATTCCTTTTCCCACATGAGTGAGGGGATTTTGATGAAAAAAGAGCCAGCAGAGGAGAG
  CACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTTCTCAAACCAGACATGCCTAATGGGTCTGGA
  AGGAATAATGACTGTGAACGGTGTTCAGATTGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAG
  GTTATGAACATGAAGAAACTCTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCA
  GGACATGACTAATTGGAAGTTGACTAAACTAAATGAGATGAATGATAGCCAAGTAAACGAAGAAAAGGAA
  AAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTTGGATTGGCAG
  ATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGTGATTTCTCCACTGTTATAGA
  CACACCAGCAGCAAATTATCTATCTAATGGTTGTGATTCCTATGGAATGCAAGACCCAGGTGTTTCTTTT
  GTTCCAAAGACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAGGAAAGCAAGTCAG
  AATGCTACTCAAATATTTATGAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAG
  CACTGGTGACCTAATGAAGAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAA
  TCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTTGGTGGTGCAAGACCCAAGCAAC
  CTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAAAATGACTTTCCTGCAAACAG
  TGGAAATAATACTAAAAATAAAAATGATATTCTTGGGAAAGCAAAATTAGGGGAAAACTCAGCAACCAAT
  GTATGCAGTCCATCTTTGGGAAACATCTCTAATGTCGATACAAATGGGGAACATTTAGAAAGTTATGAGG
  CTGAGATCTCCACTAGACCATGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGTCA
  GTTTGGAATTTCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCT
  CAGGCTCCAAATTGCATGAAATGTGAAGCCAGGTTTACATTCACCAAAAGGAGGCATCACTGCAGAGCAT
  GTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTATACATGGACAGAAAGGAAGC
  TAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGCTCAAGCCTGGGAGAACATGATGAGTGCCTCA
  AGCCAGAGCCCTAACCCTAACAATCCTGCTGAATACTGTTCTACTATCCCTCCCTTGCAGCAAGCTCAGG
  CCTCAGGAGCTCTGAGCTCTCCACCTCCCACTGTGATGGTACCTGTGGGAGTTTTAAAGCACCCTGGAGC
  AGAAGTGGCTCAGCCCAGAGAGCAGAGGCGAGTTTGGTTTGCTGATGGGATCTTGCCCAATGGAGAAGTT
  GCTGATGCAGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACCCTGGCTGTGTCACACGACCCAG
  TCAAGCCAGTAACTACCAGTCCTCTACCAGCAGAGACGGATATTTGTCTATTCTCTGGGAGTATAACTCA
  GGTTGGAAGTCCTGTTGGAAGTGCAATGAATCTTATTCCTGAAGATGGCCTTCCTCCCATTCTCATCTCC
  ACTGGTGTAAAAGGAGACTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTAATGCAGCAGTTGGAGG
  ATGGTGGCCCTGACCCACTTGTATTTGTTTTAAATGCAAATTTGTTGTCAATGGTTAAAATTGTAAATTA
  TGTGAACAGGAAGTGCTGGTGTTTCACAACCAAGGGAATGCATGCAGTGGGTCAGTCTGAGATAGTCATT
  CTTCTACAGTGTTTACCGGATGAAAAGTGTTTGCCAAAGGATATCTTTAATCACTTTGTGCAGCTTTATC
  GGGATGCTCTGGCAGGGAATGTGGTGAGCAACTTGGGACATTCCTTCTTCAGTCAAAGTTTCCTTGGCAG
  TAAAGAACATGGTGGATTCTTATATGTGACATCTACCTACCAGTCACTGCAAGACCTAGTACTCCCAACC
  CCACCTTACTTGTTTGGGATTCTTATCCAGAAATGGGAAACTCCTTGGGCTAAAGTATTTCCTATCCGTC
  TGATGTTGAGACTTGGAGCTGAATATCGACTTTATCCATGCCCACTATTCAGTGTCAGATTTCGGAAGCC
  ATTGTTTGGAGAGACGGGGCATACCATCATGAATCTTCTTGCAGACTTCAGAAATTACCAGTATACCTTG
  CCAGTAGTTCAAGGTTTGGTGGTTGATATGGAAGTTCGGAAAACTAGCATCAAAATTCCCAGCAACAGAT
  ACAATGAGATGATGAAAGCCATGAACAAGTCCAATGAGCATGTCCTGGCAGGAGGTGCCTGCTTCAATGA
  AAAGGCAGACTCTCATCTTGTGTGTGTACAGAATGATGATGGAAACTATCAGACCCAGGCTATCAGTATT
  CACAATCAGCCCAGAAAAGTGACTGGTGCCAGTTTCTTTGTGTTCAGTGGCGCTCTGAAATCCTCTTCTG
  GATACCTTGCCAAGTCCAGTATTGTGGAAGATGGTGTTATGGTCCAGATTACTGCAGAGAACATGGATTC
  CTTGAGGCAGGCACTGCGAGAGATGAAGGACTTCACCATCACCTGTGGGAAGGCGGACGCGGAGGAACCC
  CAGGAGCACATCCACATCCAGTGGGTGGATGATGACAAGAACGTTAGCAAGGGTGTCGTAAGTCCTATAG
  ATGGGAAGTCCATGGAGACTATAACAAATGTGAAGATATTCCATGGATCAGAATATAAAGCAAATGGAAA
  AGTAATCAGATGGACAGAGGTGTTTTTCCTAGAAAACGATGACCAGCACAATTGCCTCAGTGATCCTGCA
  GATCACAGTAGATTGACTGAGCATGTTGCCAAAGCTTTTTGCCTTGCTCTCTGTCCTCACCTGAAACTTC
  TGAAGGAAGATGGAATGACCAAACTGGGACTACGTGTGACACTTGACTCAGATCAGGTTGGCTATCAAGC
  AGGGAGCAATGGCCAGCCCCTTCCCTCGCAGTACATGAATGATCTGGACAGCGCCTTGGTGCCGGTGATC
  CATGGAGGGGCCTGCCAGCTTAGTGAGGGCCCCGTTGTCATGGAACTCATCTTTTATATTCTGGAAAACA
  TCGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207578 representing NM_004799
  Red=Cloning site Green=Tags(s)
  
  MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESAVSNESQPQLK
  VFSLAHSAPLTTEEEDHCANGQDCNLNPEIATMWIDENAVAEDQLIKRNYSWDDQCSAVEVGEKKCGNLA
  CLPDEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQSLMDAFSCSLDNENRQTDQFSFSINESTEKDMNSE
  KQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSPSQLKDDGSIGRDPSMSAITSLTVDSVISSQGTDGCPA
  VKKQENYIPDEDLTGKISSPRTDLGSPNSFSHMSEGILMKKEPAEESTTEESLRSGLPLLLKPDMPNGSG
  RNNDCERCSDCLVPNEVRADENEGYEHEETLGTTEFLNMTEHFSESQDMTNWKLTKLNEMNDSQVNEEKE
  KFLQISQPEDTNGDSGGQCVGLADAGLDLKGTCISESEECDFSTVIDTPAANYLSNGCDSYGMQDPGVSF
  VPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMKKNYLHNFCSQVPSVLGQ
  SSPKVVASLPSISVPFGGARPKQPSNLKLQIPKPLSDHLQNDFPANSGNNTKNKNDILGKAKLGENSATN
  VCSPSLGNISNVDTNGEHLESYEAEISTRPCLALAPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDS
  QAPNCMKCEARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSAS
  SQSPNPNNPAEYCSTIPPLQQAQASGALSSPPPTVMVPVGVLKHPGAEVAQPREQRRVWFADGILPNGEV
  ADAAKLTMNGTSSAGTLAVSHDPVKPVTTSPLPAETDICLFSGSITQVGSPVGSAMNLIPEDGLPPILIS
  TGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFVLNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQSEIVI
  LLQCLPDEKCLPKDIFNHFVQLYRDALAGNVVSNLGHSFFSQSFLGSKEHGGFLYVTSTYQSLQDLVLPT
  PPYLFGILIQKWETPWAKVFPIRLMLRLGAEYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTL
  PVVQGLVVDMEVRKTSIKIPSNRYNEMMKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISI
  HNQPRKVTGASFFVFSGALKSSSGYLAKSSIVEDGVMVQITAENMDSLRQALREMKDFTITCGKADAEEP
  QEHIHIQWVDDDKNVSKGVVSPIDGKSMETITNVKIFHGSEYKANGKVIRWTEVFFLENDDQHNCLSDPA
  DHSRLTEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQAGSNGQPLPSQYMNDLDSALVPVI
  HGGACQLSEGPVVMELIFYILENIV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004799
• ORF Size: 4275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004799.2, NP_004790.2
• RefSeq Size: 4930
• RefSeq ORF: 4278
• Locus ID: 9372
• Domains: FYVE
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]