POLE4 (NM_019896) Human Tagged ORF Clone

CAT#: RG207681

POLE4 (GFP-tagged) - Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLE4
• Synonyms: p12; YHHQ1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207681 representing NM_019896
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGCGGCAGGAAGCGGGACGCCCCGAGAGGAGGAGGTACCTGCTGGGGAGGCAGCGG
  CCTCGCAGCCCCAGGCCCCAACGAGTGTGCCTGGGGCTCGTCTCTCGAGGTTGCCTCTGGCGCGAGTGAA
  GGCCTTGGTGAAGGCAGATCCCGACGTGACGCTAGCGGGACAGGAAGCCATCTTCATTCTGGCACGAGCC
  GCGGAACTGTTTGTGGAGACCATTGCAAAAGATGCCTACTGTTGCGCTCAGCAGGGAAAAAGGAAAACCC
  TTCAGAGGAGAGACTTGGATAATGCAATAGAAGCTGTGGATGAATTTGCTTTTCTGGAAGGTACTTTAGA
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207681 representing NM_019896
  Red=Cloning site Green=Tags(s)
  
  MAAAAAAGSGTPREEEVPAGEAAASQPQAPTSVPGARLSRLPLARVKALVKADPDVTLAGQEAIFILARA
  AELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019896
• ORF Size: 351 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019896.2, NP_063949.2
• RefSeq Size: 710
• RefSeq ORF: 354
• Locus ID: 56655
• Domains: CBFD_NFYB_HMF
• Protein Pathways: Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
• Gene Summary: POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging. [supplied by OMIM, Apr 2004]