PPAN (NM_020230) Human Tagged ORF Clone

CAT#: RG207702

PPAN (GFP-tagged) - Human peter pan homolog (Drosophila) (PPAN)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPAN
• Synonyms: BXDC3; SSF; SSF-1; SSF1; SSF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207702 representing NM_020230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACAGTCAGGGAGGTCCCGGCACCAGAAGCGCGCCCGCGCCCAGGCGCAGCTCCGCAACCTCGAGG
  CCTATGCCGCGAACCCGCACTCGTTCGTGTTCACGCGAGGCTGCACGGGTCGCAACATCCGGCAGCTCAG
  CCTGGACGTGCGGCGGGTCATGGAGCCGCTCACTGCCAGCCGTCTGCAGGTTCGTAAGAAGAACTCGCTG
  AAGGACTGCGTGGCAGTGGCTGGGCCCCTCGGGGTCACACACTTTCTGATCCTGAGCAAAACAGAGACCA
  ATGTCTACTTTAAGCTGATGCGCCTCCCAGGAGGCCCCACCTTGACCTTCCAGGTCAAGAAGTACTCGCT
  GGTGCGTGATGTGGTCTCCTCACTGCGCCGGCACCGCATGCACGAGCAGCAGTTTGCCCACCCACCCCTC
  CTGGTACTCAACAGCTTTGGCCCCCATGGTATGCATGTGAAGCTCATGGCCACCATGTTCCAGAACCTGT
  TCCCCTCCATCAACGTGCACAAGGTGAACCTGAACACCATCAAGCGCTGCCTCCTCATCGACTACAACCC
  CGACTCCCAGGAGCTGGACTTCCGCCACTATAGCATCAAAGTTGTTCCTGTGGGCGCGAGTCGCGGGATG
  AAGAAGCTGCTCCAGGAGAAGTTCCCCAACATGAGCCGCCTGCAGGACATCAGCGAGCTGCTGGCCACGG
  GCGCGGGGCTGTCGGAGAGCGAGGCAGAGCCTGACGGCGACCACAACATCACAGAGCTGCCTCAGGCTGT
  CGCTGGCCGTGGCAACATGCGGGCCCAGCAGAGTGCAGTGCGGCTCACCGAGATCGGCCCGCGGATGACA
  CTGCAGCTCATCAAGGTCCAGGAGGGCGTCGGGGAGGGCAAAGTGATGTTCCACAGTTTTGTGAGCAAGA
  CGGAGGAGGAGCTGCAGGCCATCCTGGAAGCCAAGGAGAAGAAGCTGCGGCTGAAGGCGCAGAGGCAGGC
  CCAGCAGGCCCAGAATGTGCAGCGCAAGCAGGAGCAGCGGGAGGCCCACAGAAAGAAGAGCCTGGAGGGC
  ATGAAGAAGGCACGGGTCGGGGGTAGTGATGAAGAGGCCTCTGGGATCCCTTCAAGGACGGCGAGCCTGG
  AGTTGGGTGAGGACGATGATGAACAGGAAGATGATGACATCGAGTATTTCTGCCAGGCGGTGGGCGAGGC
  GCCCAGTGAGGACCTGTTCCCCGAGGCCAAGCAGAAACGGCTTGCCAAGTCTCCAGGGCGGAAGCGGAAG
  CGGTGGGAAATGGATCGAGGCAGGGGTCGCCTTTGTGACCAGAAGTTTCCCAAGACCAAGGACAAGTCCC
  AGGGAGCCCAGGCCAGGCGGGGGCCCAGAGGGGCTTCCCGGGATGGTGGGCGAGGCCGGGGCCGGGGCCG
  CCCAGGGAAGAGAGTGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207702 representing NM_020230
  Red=Cloning site Green=Tags(s)
  
  MGQSGRSRHQKRARAQAQLRNLEAYAANPHSFVFTRGCTGRNIRQLSLDVRRVMEPLTASRLQVRKKNSL
  KDCVAVAGPLGVTHFLILSKTETNVYFKLMRLPGGPTLTFQVKKYSLVRDVVSSLRRHRMHEQQFAHPPL
  LVLNSFGPHGMHVKLMATMFQNLFPSINVHKVNLNTIKRCLLIDYNPDSQELDFRHYSIKVVPVGASRGM
  KKLLQEKFPNMSRLQDISELLATGAGLSESEAEPDGDHNITELPQAVAGRGNMRAQQSAVRLTEIGPRMT
  LQLIKVQEGVGEGKVMFHSFVSKTEEELQAILEAKEKKLRLKAQRQAQQAQNVQRKQEQREAHRKKSLEG
  MKKARVGGSDEEASGIPSRTASLELGEDDDEQEDDDIEYFCQAVGEAPSEDLFPEAKQKRLAKSPGRKRK
  RWEMDRGRGRLCDQKFPKTKDKSQGAQARRGPRGASRDGGRGRGRGRPGKRVA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020230
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020230.4, NP_064615.3
• RefSeq Size: 1679
• RefSeq ORF: 1422
• Locus ID: 56342
• Domains: Brix
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation. [provided by RefSeq, Nov 2010]