TMEM219 (NM_194280) Human Tagged ORF Clone

CAT#: RG207974

TMEM219 (GFP-tagged) - Human transmembrane protein 219 (TMEM219), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM219
• Synonyms: IGFBP-3R
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207974 representing NM_194280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACTGCCAGGCAGGGCACAACCTGCACCTGTGTCTGGCCCACCACCCACCTCTGGTCTGTGCCA
  CTTTGATCCTGCTGCTCCTTGGCCTCTCTGGCCTGGGCCTTGGCAGCTTCCTCCTCACCCACAGGACTGG
  CCTGCGCAGCCCTGACATCCCCCAGGACTGGGTCTCTTTTTTGAGATCTTTTGGCCAGCTGACCCTGTGT
  CCCAGGAATGGGACAGTCACAGGGAAGTGGCGAGGGTCTCACGTCGTGGGCTTGCTGACCACCTTGAACT
  TCGGAGACGGTCCAGACAGGAACAAGACCCGGACATTCCAGGCCACAGTCCTGGGAAGTCAGATGGGATT
  GAAAGGATCTTCTGCAGGACAACTGGTCCTTATCACAGCCAGGGTGACCACAGAAAGGACTGCAGGAACC
  TGCCTATATTTTAGTGCTGTTCCAGGAATCCTACCCTCCAGCCAGCCACCCATATCCTGCTCAGAGGAGG
  GGGCTGGAAATGCCACCCTGAGCCCTAGAATGGGTGAGGAATGTGTTAGTGTCTGGAGCCATGAAGGCCT
  TGTGCTGACCAAGCTGCTCACCTCGGAGGAGCTGGCTCTGTGTGGCTCCAGGCTGCTGGTCTTGGGCTCC
  TTCCTGCTTCTCTTCTGTGGCCTTCTCTGCTGTGTCACTGCTATGTGCTTCCACCCGCGCCGGGAGTCCC
  ACTGGTCTAGAACCCGGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207974 representing NM_194280
  Red=Cloning site Green=Tags(s)
  
  MGNCQAGHNLHLCLAHHPPLVCATLILLLLGLSGLGLGSFLLTHRTGLRSPDIPQDWVSFLRSFGQLTLC
  PRNGTVTGKWRGSHVVGLLTTLNFGDGPDRNKTRTFQATVLGSQMGLKGSSAGQLVLITARVTTERTAGT
  CLYFSAVPGILPSSQPPISCSEEGAGNATLSPRMGEECVSVWSHEGLVLTKLLTSEELALCGSRLLVLGS
  FLLLFCGLLCCVTAMCFHPRRESHWSRTRL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194280
• ORF Size: 720 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_194280.2, NP_919256.1
• RefSeq Size: 1107
• RefSeq ORF: 723
• Locus ID: 124446
• Protein Families: Druggable Genome, Transmembrane