RNF149 (NM_173647) Human Tagged ORF Clone

CAT#: RG207979

RNF149 (GFP-tagged) - Human ring finger protein 149 (RNF149)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RNF149
• Synonyms: DNAPTP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207979 representing NM_173647
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGG
  CCCTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGT
  GGACCCGCAGACCAACCTGACGGTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAG
  GAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGCGGAGACCTCGAGGGCTGCGCGCCCGACA
  CGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCTCGTGGGGG
  CTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAG
  GAGCGCTACGGGAACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTA
  GCTATCCAAAAGGAAGAGAAATTTTGGAGCTGGTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGT
  TGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTGGCCATTGCCTTCATCACC
  ATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGA
  TTGGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCA
  TGGAGAAAAGGGAATTGATGTTGATGCTGAAAATTGTGCAGTGTGTATTGAAAATTTCAAAGTAAAGGAT
  ATTATTAGAATTCTGCCATGCAAGCATATTTTTCATAGAATATGCATTGACCCATGGCTTTTGGATCACC
  GAACATGTCCAATGTGTAAACTTGATGTCATCAAAGCCCTAGGATATTGGGGAGAGCCTGGGGATGTACA
  GGAGATGCCTGCTCCAGAATCTCCTCCTGGAAGGGATCCAGCTGCAAATTTGAGTCTAGCTTTACCAGAT
  GATGACGGAAGTGATGAGAGCAGTCCACCATCAGCCTCCCCTGCTGAATCTGAGCCACAGTGTGATCCCA
  GCTTTAAAGGAGATGCAGGAGAAAATACGGCATTGCTAGAAGCCGGCAGGAGTGACTCTCGGCATGGAGG
  ACCCATCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207979 representing NM_173647
  Red=Cloning site Green=Tags(s)
  
  MAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPK
  EGAHGLVGVPWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNE
  ERYGNITLPMSHAGTGNIVVIMISYPKGREILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFIT
  MMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGEKGIDVDAENCAVCIENFKVKD
  IIRILPCKHIFHRICIDPWLLDHRTCPMCKLDVIKALGYWGEPGDVQEMPAPESPPGRDPAANLSLALPD
  DDGSDESSPPSASPAESEPQCDPSFKGDAGENTALLEAGRSDSRHGGPIS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173647
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173647.2, NP_775918.1
• RefSeq Size: 2452
• RefSeq ORF: 1203
• Locus ID: 284996
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation. [UniProtKB/Swiss-Prot Function]