MEIS1 (NM_002398) Human Tagged ORF Clone

CAT#: RG208135

MEIS1 (GFP-tagged) - Human Meis homeobox 1 (MEIS1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MEIS1
• Synonyms: MGC43380
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208135 representing NM_002398
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAAAGGTACGACGATCTACCCCATTACGGGGGCATGGATGGAGTAGGCATCCCCTCCACGATGT
  ATGGGGACCCGCATGCAGCCAGGTCCATGCAGCCGGTCCACCACCTGAACCACGGGCCTCCTCTGCACTC
  GCATCAGTACCCGCACACAGCTCATACCAACGCCATGGCCCCCAGCATGGGCTCCTCTGTCAATGACGCT
  TTAAAGAGAGATAAAGATGCCATTTATGGACACCCCCTCTTCCCTCTCTTAGCACTGATTTTTGAGAAAT
  GTGAATTAGCTACTTGTACCCCCCGCGAGCCGGGGGTGGCGGGCGGGGACGTCTGCTCGTCAGAGTCATT
  CAATGAAGATATAGCCGTGTTCGCCAAACAGATTCGCGCAGAAAAACCTCTATTTTCTTCTAATCCAGAA
  CTGGATAACTTGATGATTCAAGCCATACAAGTATTAAGGTTTCATCTATTGGAATTAGAGAAGGTACACG
  AATTATGTGACAATTTCTGCCACCGGTATATTAGCTGTTTGAAAGGGAAAATGCCTATCGATTTGGTGAT
  AGACGATAGAGAAGGAGGATCAAAATCAGACAGTGAAGATATAACAAGATCAGCAAATCTAACTGACCAG
  CCCTCTTGGAACAGAGATCATGATGACACGGCATCTACTCGTTCAGGAGGAACCCCAGGCCCTTCCAGCG
  GTGGCCACACGTCACACAGTGGGGACAACAGCAGTGAGCAAGGTGATGGCTTGGACAACAGTGTAGCTTC
  CCCCAGCACAGGTGACGATGATGACCCTGATAAGGACAAAAAGCGTCACAAAAAGCGTGGCATCTTTCCC
  AAAGTAGCCACAAATATCATGAGGGCGTGGCTGTTCCAGCATCTAACACACCCTTACCCTTCTGAAGAAC
  AGAAAAAGCAGTTGGCACAAGACACGGGACTCACCATCCTTCAAGTGAACAATTGGTTTATTAATGCCCG
  GAGAAGAATAGTGCAGCCCATGATAGACCAGTCCAACCGAGCAGTAAGTCAAGGAACACCTTATAATCCT
  GATGGACAGCCCATGGGAGGTTTCGTAATGGACGGTCAGCAACATATGGGAATTAGAGCACCAGGACCTA
  TGAGTGGAATGGGCATGAATATGGGCATGGAGGGGCAGTGGCACTACATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208135 representing NM_002398
  Red=Cloning site Green=Tags(s)
  
  MAQRYDDLPHYGGMDGVGIPSTMYGDPHAARSMQPVHHLNHGPPLHSHQYPHTAHTNAMAPSMGSSVNDA
  LKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQIRAEKPLFSSNPE
  LDNLMIQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDDREGGSKSDSEDITRSANLTDQ
  PSWNRDHDDTASTRSGGTPGPSSGGHTSHSGDNSSEQGDGLDNSVASPSTGDDDDPDKDKKRHKKRGIFP
  KVATNIMRAWLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVSQGTPYNP
  DGQPMGGFVMDGQQHMGIRAPGPMSGMGMNMGMEGQWHYM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002398
• ORF Size: 1170 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002398.2, NP_002389.1
• RefSeq Size: 3198 bp
• RefSeq ORF: 1173 bp
• Locus ID: 4211
• Cytogenetics: 2p14
• Domains: homeobox
• Protein Families: Transcription Factors
• Gene Summary: 'Homeobox genes, of which the most well-characterized category is represented by the HOX genes, play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. [provided by RefSeq, Jul 2008]'