Syntaxin 2 (STX2) (NM_001980) Human Tagged ORF Clone

CAT#: RG208207

STX2 (GFP-tagged) - Human syntaxin 2 (STX2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: STX2
• Synonyms: EPIM; EPM; STX2A; STX2B; STX2C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208207 representing NM_001980
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGACCGGCTGCCAGACCTGACGGCGTGTAGGAAGAATGATGATGGAGACACAGTTGTTGTGGTTG
  AGAAAGATCATTTCATGGATGATTTCTTCCATCAGGTGGAGGAGATTAGAAACAGTATTGATAAAATAAC
  TCAATATGTTGAAGAAGTAAAGAAAAACCACAGCATCATTCTTTCTGCACCAAACCCGGAAGGAAAAATA
  AAAGAAGAGCTTGAAGATCTGAACAAAGAAATCAAGAAAACTGCGAATAAAATTCGAGCCAAGTTAAAGG
  CTATTGAACAAAGTTTTGATCAGGATGAGAGTGGGAACCGGACTTCAGTGGATCTTCGGATACGAAGAAC
  CCAGCATTCGGTGCTGTCTCGGAAGTTTGTGGAAGCCATGGCGGAGTACAATGAGGCACAGACTCTGTTT
  CGGGAGCGGAGCAAAGGCCGCATCCAGCGCCAGCTGGAGATAACTGGGAGAACCACCACAGACGACGAGC
  TAGAAGAGATGCTGGAGAGCGGGAAGCCATCCATCTTCACTTCCGACATTATATCAGATTCACAAATTAC
  TAGACAAGCTCTCAATGAAATCGAGTCACGTCACAAGGACATCATGAAGCTGGAGACCAGCATCCGAGAG
  TTGCATGAGATGTTCATGGACATGGCTATGTTTGTGGAGACTCAGGGTGAAATGATCAACAACATAGAAA
  GAAATGTTATGAATGCCACAGACTATGTAGAACACGCTAAAGAAGAAACAAAAAAAGCTATCAAATATCA
  GAGCAAGGCAAGAAGGAAATTGATGTTCATTATTATTTGTGTAATTGTTTTGCTTGTGATCCTTGGAATT
  ATCCTAGCAACAACATTGTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208207 representing NM_001980
  Red=Cloning site Green=Tags(s)
  
  MRDRLPDLTACRKNDDGDTVVVVEKDHFMDDFFHQVEEIRNSIDKITQYVEEVKKNHSIILSAPNPEGKI
  KEELEDLNKEIKKTANKIRAKLKAIEQSFDQDESGNRTSVDLRIRRTQHSVLSRKFVEAMAEYNEAQTLF
  RERSKGRIQRQLEITGRTTTDDELEEMLESGKPSIFTSDIISDSQITRQALNEIESRHKDIMKLETSIRE
  LHEMFMDMAMFVETQGEMINNIERNVMNATDYVEHAKEETKKAIKYQSKARRKLMFIIICVIVLLVILGI
  ILATTLS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001980
• ORF Size: 861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001980.2, NP_001971.2
• RefSeq Size: 3343 bp
• RefSeq ORF: 864 bp
• Locus ID: 2054
• Cytogenetics: 12q24.33
• Domains: t_SNARE, SynN
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'The product of this gene belongs to the syntaxin/epimorphin family of proteins. The syntaxins are a large protein family implicated in the targeting and fusion of intracellular transport vesicles. The product of this gene regulates epithelial-mesenchymal interactions and epithelial cell morphogenesis and activation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'