KMT5A (NM_020382) Human Tagged ORF Clone

CAT#: RG208219

SETD8 (GFP-tagged) - Human SET domain containing (lysine methyltransferase) 8 (SETD8)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KMT5A
• Synonyms: PR-Set7; PR/SET07; SET07; SET8; SETD8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208219 representing NM_020382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGAGGCAGGAAGATGTCCAAGCCCCGCGCGGTGGAGGCGGCGGCGGCGGCGGCGGCGGTGGCAG
  CGACGGCCCCGGGCCCGGAGATGGTGGAGCGGAGGGGCCCGGGGAGGCCCCGCACCGACGGGGAGAACGT
  ATTTACCGGGCAGTCAAAGATCTATTCCTACATGAGCCCGAACAAATGCTCTGGAATGCGTTTCCCCCTT
  CAGGAAGAGAACTCAGTTACACATCACGAAGTCAAATGCCAGGGGAAACCATTAGCCGGAATCTACAGGA
  AACGAGAAGAGAAAAGAAATGCTGGGAACGCAGTACGGAGCGCCATGAAGTCCGAGGAACAGAAGATCAA
  AGACGCCAGGAAAGGTCCCCTGGTACCTTTTCCAAACCAAAAATCTGAAGCAGCAGAACCTCCAAAAACT
  CCACCCTCATCTTGTGATTCCACCAATGCAGCCATCGCCAAGCAAGCCCTGAAAAAGCCCATCAAGGGCA
  AACAGGCCCCCCGAAAAAAAGCTCAAGGAAAAACGCAACAGAATCGCAAACTTACGGATTTCTACCCTGT
  CCGAAGGAGCTCCAGGAAGAGCAAAGCCGAGCTGCAGTCTGAAGAAAGGAAAAGAATAGATGAATTGATT
  GAAAGTGGGAAGGAAGAAGGAATGAAGATTGACCTCATCGATGGCAAAGGCAGGGGTGTGATTGCCACCA
  AGCAGTTCTCCCGGGGTGACTTTGTGGTGGAATACCACGGGGACCTCATCGAGATCACCGACGCCAAGAA
  ACGGGAGGCTCTGTACGCACAGGACCCTTCCACGGGCTGCTACATGTACTATTTTCAGTATCTGAGCAAA
  ACCTACTGCGTGGATGCAACTAGAGAGACAAATCGCCTAGGAAGACTGATCAATCACAGCAAATGTGGGA
  ACTGCCAAACCAAACTGCACGACATCGACGGCGTACGTCACCTCATCCTCATCGCCTCCCGAGACATCGC
  GGCTGGGGAGGAGCTCCTGTATGACTATGGGGACCGCAGCAAGGCTTCCATTGAAGCCCACCCGTGGCTG
  AAGCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208219 representing NM_020382
  Red=Cloning site Green=Tags(s)
  
  MARGRKMSKPRAVEAAAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMSPNKCSGMRFPL
  QEENSVTHHEVKCQGKPLAGIYRKREEKRNAGNAVRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKT
  PPSSCDSTNAAIAKQALKKPIKGKQAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELI
  ESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSK
  TYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVRHLILIASRDIAAGEELLYDYGDRSKASIEAHPWL
  KH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020382
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020382.3, NP_065115.3
• RefSeq Size: 2765
• RefSeq ORF: 1059
• Locus ID: 387893
• Domains: SET
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• Gene Summary: The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation. [provided by RefSeq, May 2016]