Topoisomerase III alpha (TOP3A) (NM_004618) Human Tagged ORF Clone

CAT#: RG208236

TOP3A (GFP-tagged) - Human topoisomerase (DNA) III alpha (TOP3A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TOP3A
• Synonyms: MGRISCE2; PEOB5; TOP3; ZGRF7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208236 representing NM_004618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTTTCCTGTCGCCCGCTACGCGCTCCGGTGGCTGCGACGGCCCGAAGACCGTGCCTTTTCCCGCG
  CCGCCATGGAGATGGCCCTCCGAGGCGTGCGGAAAGTCCTCTGTGTGGCCGAAAAAAACGACGCGGCCAA
  GGGGATCGCCGACCTGCTGTCAAACGGTCGCATGAGGCGGAGAGAAGGACTTTCAAAATTCAACAAGATC
  TATGAATTTGATTATCATCTGTATGGCCAGAATGTTACCATGGTAATGACTTCAGTTTCTGGACATTTAC
  TGGCTCATGATTTCCAGATGCAGTTTCGAAAATGGCAGAGCTGCAACCCTCTTGTCCTCTTTGAAGCAGA
  AATTGAAAAGTACTGCCCAGAGAATTTTGTAGACATCAAGAAAACTTTGGAACGAGAGACTCGCCAGTGC
  CAGGCTCTGGTGATCTGGACTGACTGTGATAGAGAAGGCGAAAACATCGGGTTTGAGATTATCCACGTGT
  GTAAGGCTGTAAAGCCCAATCTGCAGGTGTTGCGAGCCCGATTCTCTGAGATCACACCCCATGCCGTCAG
  GACAGCTTGTGAAAACCTGACCGAGCCTGATCAGAGGGTGAGCGATGCTGTGGATGTGAGGCAGGAGCTG
  GACCTGAGGATTGGAGCTGCCTTTACTAGGTTCCAGACCCTGCGGCTTCAGAGGATTTTTCCTGAGGTGC
  TGGCAGAGCAGCTCATCAGTTACGGCAGCTGCCAGTTCCCCACACTGGGCTTTGTGGTGGAGCGGTTCAA
  AGCCATTCAGGCTTTTGTACCAGAAATCTTCCACAGAATTAAAGTAACTCATGACCACAAAGATGGTATC
  GTAGAATTCAACTGGAAAAGGCATCGACTCTTTAACCACACGGCTTGCCTAGTTCTCTATCAGTTGTGTG
  TGGAGGATCCCATGGCAACTGTGGTAGAGGTCAGATCTAAGCCCAAGAGCAAGTGGCGGCCTCAAGCCTT
  GGACACTGTGGAGCTTGAGAAGCTGGCTTCTCGAAAGTTGAGAATAAATGCTAAAGAAACCATGAGGATT
  GCTGAGAAGCTCTACACTCAAGGGTACATCAGCTATCCCCGAACAGAAACAAACATTTTTCCCAGAGACT
  TAAACCTGACGGTGTTGGTGGAACAGCAGACCCCCGATCCACGCTGGGGGGCCTTTGCCCAGAGCATTCT
  AGAGCGGGGTGGTCCCACCCCACGCAATGGGAACAAGTCTGACCAAGCTCACCCTCCCATTCACCCCACC
  AAATACACCAACAACTTACAGGGAGATGAACAGCGACTGTACGAGTTTATTGTTCGCCATTTCCTGGCTT
  GCTGCTCCCAGGATGCTCAGGGGCAGGAGACCACAGTGGAGATCGACATCGCTCAGGAACGCTTTGTGGC
  CCATGGCCTCATGATTCTGGCCCGAAACTATCTGGATGTGTATCCATATGATCACTGGAGTGACAAGATC
  CTCCCTGTCTATGAGCAAGGATCCCACTTTCAGCCCAGCACCGTGGAGATGGTGGACGGGGAGACCAGCC
  CACCCAAGCTGCTCACCGAGGCCGACCTCATTGCCCTCATGGAGAAGCATGGCATTGGTACGGATGCCAC
  TCATGCGGAGCACATCGAGACCATCAAAGCCCGGATGTACGTGGGCCTCACCCCAGACAAGCGGTTCCTC
  CCTGGGCACCTGGGCATGGGACTTGTGGAAGGTTATGATTCCATGGGCTATGAAATGTCTAAGCCTGACC
  TCCGGGCTGAACTGGAAGCTGATCTGAAGCTGATCTGTGATGGCAAAAAGGACAAATTTGTGGTTCTAAG
  GCAGCAAGTGCAGAAATACAAGCAGGTTTTCATTGAAGCGGTGGCTAAAGCAAAGAAATTGGACGAGGCC
  TTGGCCCAGTACTTTGGGAATGGGACAGAGTTGGCCCAGCAAGAAGATATCTACCCAGCCATGCCAGAGC
  CCATCAGGAAGTGCCCACAGTGCAACAAGGACATGGTCCTTAAGACCAAGAAGAATGGCGGGTTCTACCT
  CAGCTGCATGGGTTTCCCAGAGTGTCGCTCAGCTGTGTGGCTTCCTGACTCGGTGCTGGAGGCCAGCAGG
  GACAGCAGTGTGTGTCCAGTTTGTCAGCCACACCCTGTGTACAGGTTAAAGTTAAAGTTTAAGCGCGGTA
  GCCTTCCCCCGACCATGCCTCTGGAGTTTGTTTGCTGCATCGGCGGATGCGACGACACCCTGAGGGAGAT
  CCTGGACCTGAGATTTTCAGGGGGCCCCCCCAGGGCTAGCCAGCCCTCTGGCCGCCTGCAGGCTAACCAG
  TCCCTGAACAGGATGGACAACAGCCAGCACCCCCAGCCTGCTGACAGCAGACAGACTGGGTCCTCAAAGG
  CTCTGGCCCAGACCCTCCCACCACCCACGGCTGCTGGTGAAAGCAATTCTGTGACCTGCAACTGTGGCCA
  GGAGGCTGTGCTGCTCACTGTCCGTAAGGAGGGCCCCAACCGGGGCCGGCAGTTCTTTAAGTGCAACGGA
  GGTAGCTGCAACTTCTTCCTGTGGGCAGACAGCCCCAATCCGGGAGCAGGAGGGCCTCCTGCCTTGGCAT
  ATAGACCCCTGGGCGCCTCCCTGGGATGCCCACCAGGCCCAGGGATCCACCTAGGTGGGTTTGGCAACCC
  TGGTGATGGCAGTGGTAGTGGCACATCCTGCCTTTGCAGCCAGCCCTCCGTCACACGGACTGTGCAGAAG
  GATGGACCCAACAAGGGGCGCCAGTTCCACACATGTGCCAAGCCGAGAGAGCAGCAGTGTGGCTTTTTCC
  AGTGGGTCGATGAGAACACCGCTCCAGGGACTTCTGGAGCCCCGTCCTGGACAGGAGACAGAGGAAGAAC
  CCTGGAGTCGGAAGCCAGAAGCAAAAGGCCCCGGGCCAGTTCCTCAGACATGGGGTCCACAGCAAAGAAA
  CCCCGGAAATGCAGCCTTTGCCACCAGCCTGGACACACCCGTCCCTTTTGTCCTCAGAACAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208236 representing NM_004618
  Red=Cloning site Green=Tags(s)
  
  MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKI
  YEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQC
  QALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQEL
  DLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGI
  VEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRI
  AEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPT
  KYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKI
  LPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFL
  PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEA
  LAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASR
  DSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQ
  SLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNG
  GSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQK
  DGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKK
  PRKCSLCHQPGHTRPFCPQNR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004618
• ORF Size: 3003 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004618.3, NP_004609.1
• RefSeq Size: 4116 bp
• RefSeq ORF: 3006 bp
• Locus ID: 7156
• Cytogenetics: 17p11.2
• Domains: Topoisom_bac, zf-CCHC, TOP1Bc, TOP1Ac, TOPRIM, Toprim
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination
• Gene Summary: 'This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]'