Topoisomerase III alpha (TOP3A) (NM_004618) Human Tagged ORF Clone
CAT#: RG208236
- TrueORF®
TOP3A (GFP-tagged) - Human topoisomerase (DNA) III alpha (TOP3A)
"NM_004618" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | TOP3A |
Synonyms | MGRISCE2; PEOB5; TOP3; ZGRF7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208236 representing NM_004618
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTTTCCTGTCGCCCGCTACGCGCTCCGGTGGCTGCGACGGCCCGAAGACCGTGCCTTTTCCCGCG CCGCCATGGAGATGGCCCTCCGAGGCGTGCGGAAAGTCCTCTGTGTGGCCGAAAAAAACGACGCGGCCAA GGGGATCGCCGACCTGCTGTCAAACGGTCGCATGAGGCGGAGAGAAGGACTTTCAAAATTCAACAAGATC TATGAATTTGATTATCATCTGTATGGCCAGAATGTTACCATGGTAATGACTTCAGTTTCTGGACATTTAC TGGCTCATGATTTCCAGATGCAGTTTCGAAAATGGCAGAGCTGCAACCCTCTTGTCCTCTTTGAAGCAGA AATTGAAAAGTACTGCCCAGAGAATTTTGTAGACATCAAGAAAACTTTGGAACGAGAGACTCGCCAGTGC CAGGCTCTGGTGATCTGGACTGACTGTGATAGAGAAGGCGAAAACATCGGGTTTGAGATTATCCACGTGT GTAAGGCTGTAAAGCCCAATCTGCAGGTGTTGCGAGCCCGATTCTCTGAGATCACACCCCATGCCGTCAG GACAGCTTGTGAAAACCTGACCGAGCCTGATCAGAGGGTGAGCGATGCTGTGGATGTGAGGCAGGAGCTG GACCTGAGGATTGGAGCTGCCTTTACTAGGTTCCAGACCCTGCGGCTTCAGAGGATTTTTCCTGAGGTGC TGGCAGAGCAGCTCATCAGTTACGGCAGCTGCCAGTTCCCCACACTGGGCTTTGTGGTGGAGCGGTTCAA AGCCATTCAGGCTTTTGTACCAGAAATCTTCCACAGAATTAAAGTAACTCATGACCACAAAGATGGTATC GTAGAATTCAACTGGAAAAGGCATCGACTCTTTAACCACACGGCTTGCCTAGTTCTCTATCAGTTGTGTG TGGAGGATCCCATGGCAACTGTGGTAGAGGTCAGATCTAAGCCCAAGAGCAAGTGGCGGCCTCAAGCCTT GGACACTGTGGAGCTTGAGAAGCTGGCTTCTCGAAAGTTGAGAATAAATGCTAAAGAAACCATGAGGATT GCTGAGAAGCTCTACACTCAAGGGTACATCAGCTATCCCCGAACAGAAACAAACATTTTTCCCAGAGACT TAAACCTGACGGTGTTGGTGGAACAGCAGACCCCCGATCCACGCTGGGGGGCCTTTGCCCAGAGCATTCT AGAGCGGGGTGGTCCCACCCCACGCAATGGGAACAAGTCTGACCAAGCTCACCCTCCCATTCACCCCACC AAATACACCAACAACTTACAGGGAGATGAACAGCGACTGTACGAGTTTATTGTTCGCCATTTCCTGGCTT GCTGCTCCCAGGATGCTCAGGGGCAGGAGACCACAGTGGAGATCGACATCGCTCAGGAACGCTTTGTGGC CCATGGCCTCATGATTCTGGCCCGAAACTATCTGGATGTGTATCCATATGATCACTGGAGTGACAAGATC CTCCCTGTCTATGAGCAAGGATCCCACTTTCAGCCCAGCACCGTGGAGATGGTGGACGGGGAGACCAGCC CACCCAAGCTGCTCACCGAGGCCGACCTCATTGCCCTCATGGAGAAGCATGGCATTGGTACGGATGCCAC TCATGCGGAGCACATCGAGACCATCAAAGCCCGGATGTACGTGGGCCTCACCCCAGACAAGCGGTTCCTC CCTGGGCACCTGGGCATGGGACTTGTGGAAGGTTATGATTCCATGGGCTATGAAATGTCTAAGCCTGACC TCCGGGCTGAACTGGAAGCTGATCTGAAGCTGATCTGTGATGGCAAAAAGGACAAATTTGTGGTTCTAAG GCAGCAAGTGCAGAAATACAAGCAGGTTTTCATTGAAGCGGTGGCTAAAGCAAAGAAATTGGACGAGGCC TTGGCCCAGTACTTTGGGAATGGGACAGAGTTGGCCCAGCAAGAAGATATCTACCCAGCCATGCCAGAGC CCATCAGGAAGTGCCCACAGTGCAACAAGGACATGGTCCTTAAGACCAAGAAGAATGGCGGGTTCTACCT CAGCTGCATGGGTTTCCCAGAGTGTCGCTCAGCTGTGTGGCTTCCTGACTCGGTGCTGGAGGCCAGCAGG GACAGCAGTGTGTGTCCAGTTTGTCAGCCACACCCTGTGTACAGGTTAAAGTTAAAGTTTAAGCGCGGTA GCCTTCCCCCGACCATGCCTCTGGAGTTTGTTTGCTGCATCGGCGGATGCGACGACACCCTGAGGGAGAT CCTGGACCTGAGATTTTCAGGGGGCCCCCCCAGGGCTAGCCAGCCCTCTGGCCGCCTGCAGGCTAACCAG TCCCTGAACAGGATGGACAACAGCCAGCACCCCCAGCCTGCTGACAGCAGACAGACTGGGTCCTCAAAGG CTCTGGCCCAGACCCTCCCACCACCCACGGCTGCTGGTGAAAGCAATTCTGTGACCTGCAACTGTGGCCA GGAGGCTGTGCTGCTCACTGTCCGTAAGGAGGGCCCCAACCGGGGCCGGCAGTTCTTTAAGTGCAACGGA GGTAGCTGCAACTTCTTCCTGTGGGCAGACAGCCCCAATCCGGGAGCAGGAGGGCCTCCTGCCTTGGCAT ATAGACCCCTGGGCGCCTCCCTGGGATGCCCACCAGGCCCAGGGATCCACCTAGGTGGGTTTGGCAACCC TGGTGATGGCAGTGGTAGTGGCACATCCTGCCTTTGCAGCCAGCCCTCCGTCACACGGACTGTGCAGAAG GATGGACCCAACAAGGGGCGCCAGTTCCACACATGTGCCAAGCCGAGAGAGCAGCAGTGTGGCTTTTTCC AGTGGGTCGATGAGAACACCGCTCCAGGGACTTCTGGAGCCCCGTCCTGGACAGGAGACAGAGGAAGAAC CCTGGAGTCGGAAGCCAGAAGCAAAAGGCCCCGGGCCAGTTCCTCAGACATGGGGTCCACAGCAAAGAAA CCCCGGAAATGCAGCCTTTGCCACCAGCCTGGACACACCCGTCCCTTTTGTCCTCAGAACAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208236 representing NM_004618
Red=Cloning site Green=Tags(s) MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKI YEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQC QALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQEL DLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGI VEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRI AEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPT KYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKI LPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFL PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEA LAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASR DSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQ SLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNG GSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQK DGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKK PRKCSLCHQPGHTRPFCPQNR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004618 |
ORF Size | 3003 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004618.3, NP_004609.1 |
RefSeq Size | 4116 bp |
RefSeq ORF | 3006 bp |
Locus ID | 7156 |
Cytogenetics | 17p11.2 |
Domains | Topoisom_bac, zf-CCHC, TOP1Bc, TOP1Ac, TOPRIM, Toprim |
Protein Families | Druggable Genome |
Protein Pathways | Homologous recombination |
Gene Summary | 'This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117267 | TOP3A (untagged)-Human topoisomerase (DNA) III alpha (TOP3A) |
USD 310.00 |
|
RC208236 | TOP3A (Myc-DDK-tagged)-Human topoisomerase (DNA) III alpha (TOP3A) |
USD 1,050.00 |
|
RC208236L3 | Lenti-ORF clone of TOP3A (Myc-DDK-tagged)-Human topoisomerase (DNA) III alpha (TOP3A) |
USD 1,250.00 |
|
RC208236L4 | Lenti-ORF clone of TOP3A (mGFP-tagged)-Human topoisomerase (DNA) III alpha (TOP3A) |
USD 1,548.00 |
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