RNF156 (MGRN1) (NM_015246) Human Tagged ORF Clone

CAT#: RG208284

MGRN1 (GFP-tagged) - Human mahogunin, ring finger 1 (MGRN1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MGRN1
• Synonyms: RNF156
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208284 representing NM_015246
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCATTCTCAGCCGCCGCATCGCGGGGGTGGAGGACATCGACATCCAGGCGAACTCGGCCTATC
  GCTACCCTCCGAAGTCCGGAAACTACTTTGCTTCGCACTTTTTCATGGGAGGAGAGAAATTCGACACCCC
  CCACCCTGAAGGTTACCTCTTTGGAGAGAACATGGATCTGAACTTCCTGGGCAGCCGCCCGGTCCAGTTT
  CCCTACGTCACTCCTGCCCCCCACGAGCCCGTGAAGACGCTGCGGAGCCTGGTGAACATCCGCAAAGACT
  CCCTGCGGCTGGTGAGGTACAAAGACGATGCCGACAGCCCCACCGAGGACGGCGACAAGCCCCGGGTGCT
  CTACAGCCTGGAGTTCACCTTCGACGCCGATGCCCGCGTGGCCATCACCATCTACTGCCAGGCATCGGAG
  GAGTTCCTGAACGGCAGGGCAGTATACAGCCCCAAGAGCCCCTCGCTACAGTCCGAGACCGTCCACTACA
  AGAGAGGGGTGAGCCAGCAGTTCTCCCTGCCCTCCTTCAAGATTGACTTCTCGGAATGGAAGGATGACGA
  GCTGAACTTTGACCTGGACCGGGGCGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGAGATGTG
  GTGGAAGTGACTGGCCACGCCCACGTGCTCTTGGCTGCCTTTGAAAAGCACATGGACGGCAGCTTCTCTG
  TGAAGCCTTTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTCCTGCAGGAGATCTATGGCATTGA
  GAACAAGAACAACCAGGAGACCAAGCCCTCGGACGACGAGAACAGCGACAACAGCAACGAGTGTGTGGTG
  TGCCTGTCCGACCTGCGGGACACGCTGATCCTGCCCTGCCGCCACCTGTGCCTCTGTACCTCCTGCGCCG
  ACACGCTGCGCTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCTTTCCGGGCCCTCCTGCAGATCCG
  GGCGGTGCGGAAGAAGCCAGGAGCCCTGTCCCCCGTGTCCTTCAGCCCCGTCCTGGCCCAGAGCCTGGAG
  CATGATGAGCACTCTTGTCCCTTTAAAAAATCAAAGCCGCACCCCGCCTCCCTGGCCAGCAAGAAACCTA
  AAAGGGAAACAAACTCTGACAGCGTCCCACCTGGCTACGAGCCCATCTCGCTGCTCGAGGCGCTCAACGG
  CCTCCGGGCTGTCTCCCCGGCCATCCCCTCGGCCCCTCTTTATGAAGAAATCACCTATTCAGGCATCTCG
  GACGGCCTGTCCCAGGCCAGCTGTCCCCTCGCGGCTATCGACCACATCCTGGACAGCAGCCGCCAGAAGG
  GCAGGCCGCAGAGCAAGGCCCCCGACAGCACCCTACGGTCCCCGTCTTCCCCCATCCACGAAGAGGATGA
  GGAGAAGCTCTCCGAGGACGTGGACGCCCCTCCCCCACTGGGTGGCGCAGAGCTGGCCCTGCGGGAAAGC
  AGCTCCCCTGAGAGTTTCATAACAGAAGAGGTTGATGAGTCGTCGTCACCACAGCAAGGGACCCGAGCAG
  CTTCCATTGAGAATGTCCTGCAGGACAGCAGCCCCGAGCACTGTGGCCGAGGCCCACCTGCTGACATCTA
  CCTGCCAGGACGGCCCACCTCCATGGAGACGGCCCACGGCCTCGCCACCACCAGCCCCACCTGGCCTCCA
  CTTGGTGGCCCCAGCCCCGATCCCAGCGCCGCCGAGCTGACCCCACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208284 representing NM_015246
  Red=Cloning site Green=Tags(s)
  
  MGSILSRRIAGVEDIDIQANSAYRYPPKSGNYFASHFFMGGEKFDTPHPEGYLFGENMDLNFLGSRPVQF
  PYVTPAPHEPVKTLRSLVNIRKDSLRLVRYKDDADSPTEDGDKPRVLYSLEFTFDADARVAITIYCQASE
  EFLNGRAVYSPKSPSLQSETVHYKRGVSQQFSLPSFKIDFSEWKDDELNFDLDRGVFPVVIQAVVDEGDV
  VEVTGHAHVLLAAFEKHMDGSFSVKPLKQKQIVDRVSYLLQEIYGIENKNNQETKPSDDENSDNSNECVV
  CLSDLRDTLILPCRHLCLCTSCADTLRYQANNCPICRLPFRALLQIRAVRKKPGALSPVSFSPVLAQSLE
  HDEHSCPFKKSKPHPASLASKKPKRETNSDSVPPGYEPISLLEALNGLRAVSPAIPSAPLYEEITYSGIS
  DGLSQASCPLAAIDHILDSSRQKGRPQSKAPDSTLRSPSSPIHEEDEEKLSEDVDAPPPLGGAELALRES
  SSPESFITEEVDESSSPQQGTRAASIENVLQDSSPEHCGRGPPADIYLPGRPTSMETAHGLATTSPTWPP
  LGGPSPDPSAAELTPL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015246
• ORF Size: 1728 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015246.1, NP_056061.1
• RefSeq Size: 3941
• RefSeq ORF: 1731
• Locus ID: 23295
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro. [supplied by OMIM, Apr 2004]