HS3ST3A1 (NM_006042) Human Tagged ORF Clone

CAT#: RG208327

HS3ST3A1 (GFP-tagged) - Human heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 (HS3ST3A1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HS3ST3A1
• Synonyms: 3-OST-3A; 3OST3A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208327 representing NM_006042
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTCCGGGCCCGGCCAGTGCCCTCTCCACCTCGGCCGAGCCGCTGTCCCGCAGCATCTTCCGGA
  AGTTCTTGCTGATGCTCTGCTCCCTGCTCACGTCCCTTTACGTCTTCTACTGCCTGGCCGAGCGCTGCCA
  GACCCTGTCCGGCCCCGTCGTGGGGCTGTCCGGCGGCGGCGAGGAGGCGGGGGCCCCTGGTGGCGGCGTC
  CTGGCCGGAGGCCCGAGGGAGCTGGCGGTGTGGCCGGCGGCGGCACAGAGAAAGCGCCTCCTGCAACTGC
  CGCAGTGGCGGAGGCGCCGGCCGCCCGCGCCCCGCGACGACGGCGAGGAGGCGGCCTGGGAAGAAGAGTC
  CCCTGGCCTGTCAGGGGGTCCGGGCGGCTCCGGGGCCGGAAGCACCGTGGCCGAGGCCCCGCCGGGGACC
  CTGGCGCTGCTCCTGGACGAAGGCAGCAAGCAGCTGCCGCAGGCCATCATCATCGGAGTGAAGAAGGGCG
  GCACGCGGGCGCTGCTGGAGTTCCTGCGCGTGCACCCCGACGTGCGCGCCGTGGGCGCCGAGCCCCACTT
  CTTCGACCGCAGCTACGACAAGGGCCTCGCCTGGTACCGGGACCTGATGCCCAGAACCCTGGACGGGCAG
  ATCACCATGGAGAAGACGCCCAGTTACTTCGTCACGCGGGAGGCCCCCGCGCGCATCTCGGCCATGTCCA
  AGGACACCAAGCTCATCGTGGTGGTGCGGGACCCGGTGACCAGGGCCATCTCGGACTACACGCAGACGCT
  GTCCAAGCGGCCCGACATCCCCACCTTCGAGAGCTTGACGTTCAAAAACAGGACAGCGGGCCTCATCGAC
  ACGTCGTGGAGCGCCATCCAGATCGGCATCTACGCCAAGCACCTGGAGCACTGGCTGCGCCACTTCCCCA
  TCCGCCAGATGCTCTTCGTGAGCGGCGAGCGGCTCATCAGCGACCCGGCCGGGGAGCTGGGCCGCGTGCA
  AGACTTCCTGGGCCTCAAGAGGATCATCACGGACAAGCACTTCTACTTCAACAAGACCAAGGGCTTCCCC
  TGCCTGAAGAAGGCGGAGGGCAGCAGCCGGCCCCATTGCCTGGGCAAGACCAAGGGCAGGACCCATCCTG
  AGATCGACCGCGAGGTGGTGCGCAGGCTGCGCGAGTTCTACCGGCCTTTCAACCTCAAGTTCTACCAGAT
  GACCGGGCACGACTTTGGCTGGGATGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208327 representing NM_006042
  Red=Cloning site Green=Tags(s)
  
  MAPPGPASALSTSAEPLSRSIFRKFLLMLCSLLTSLYVFYCLAERCQTLSGPVVGLSGGGEEAGAPGGGV
  LAGGPRELAVWPAAAQRKRLLQLPQWRRRRPPAPRDDGEEAAWEEESPGLSGGPGGSGAGSTVAEAPPGT
  LALLLDEGSKQLPQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRSYDKGLAWYRDLMPRTLDGQ
  ITMEKTPSYFVTREAPARISAMSKDTKLIVVVRDPVTRAISDYTQTLSKRPDIPTFESLTFKNRTAGLID
  TSWSAIQIGIYAKHLEHWLRHFPIRQMLFVSGERLISDPAGELGRVQDFLGLKRIITDKHFYFNKTKGFP
  CLKKAEGSSRPHCLGKTKGRTHPEIDREVVRRLREFYRPFNLKFYQMTGHDFGWDG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006042
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006042.1, NP_006033.1
• RefSeq Size: 2546
• RefSeq ORF: 1221
• Locus ID: 9955
• Protein Pathways: Glycosaminoglycan degradation, Heparan sulfate biosynthesis
• Gene Summary: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. The sulfotransferase domain of this enzyme is highly similar to the same domain of heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1, and these two enzymes sulfate an identical disaccharide. This gene is widely expressed, with the most abundant expression in liver and placenta. [provided by RefSeq, Dec 2014]