SMARCAD1 (NM_020159) Human Tagged ORF Clone

CAT#: RG208438

SMARCAD1 (GFP-tagged) - Human SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 (SMARCAD1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SMARCAD1
• Synonyms: ADERM; BASNS; ETL1; HEL1; HRZ
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208438 representing NM_020159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTTTCAACCTGGACCGTTTTCGCTTTGAGAAAAGGAATAAGATTGAGGAAGCGCCCGAAGCAA
  CCCCTCAACCTTCCCAGCCTGGCCCTTCTTCACCAATTTCTCTTAGTGCTGAAGAGGAGAATGCTGAAGG
  GGAAGTTAGCAGGGCAAACACTCCTGATTCAGATATAACTGAAAAAACAGAAGATTCTAGTGTTCCAGAA
  ACTCCAGATAATGAAAGAAAAGCAAGTATATCATATTTCAAAAATCAAAGAGGAATACAGTATATTGATT
  TGTCTTCTGATAGTGAAGATGTCGTTTCCCCAAATTGCTCCAATACAGTTCAAGAGAAAACATTCAACAA
  AGATACAGTGATTATAGTTTCTGAGCCATCTGAAGATGAAGAGTCCCAAGGCCTTCCTACCATGGCACGT
  AGAAATGATGATATTTCAGAACTGGAAGACCTTTCGGAATTGGAAGACCTTAAAGATGCTAAACTTCAGA
  CTTTGAAGGAACTTTTTCCACAAAGAAGTGACAATGATTTACTTAAGTTGATTGAATCAACAAGCACTAT
  GGATGGAGCAATTGCTGCTGCCTTGCTGATGTTTGGTGATGCAGGTGGTGGGCCCAGGAAAAGAAAATTA
  TCTTCTTCTTCAGAGCCATATGAGGAAGATGAATTTAATGATGATCAATCTATAAAAAAGACAAGACTGG
  ATCATGGAGAGGAATCAAATGAGTCTGCAGAATCTAGCAATAATTGGGAAAAGCAGGAAAGTATTGTACT
  GAAATTGCAAAAGGAATTTCCCAATTTTGATAAACAGGAACTTAGAGAAGTACTCAAGGAACATGAATGG
  ATGTACACAGAAGCTTTAGAATCTCTAAAAGTGTTTGCAGAAGACCAAGATATGCAATATGCATCACAAA
  GTGAGGTTCCAAATGGAAAAGAAGTTTCTTCAAGAAGTCAAAATTACCCTAAAAATGCAACTAAAACAAA
  ACTAAAACAGAAATTTTCAATGAAAGCACAAAATGGCTTTAACAAGAAACGTAAAAAAAATGTTTTTAAT
  CAAAAGAGAGTTGTTGAAGACTCTGAATATGATTCAGGTTCTGATGTCGGTAGTTCACTAGATGAGGACT
  ATAGTAGTGGTGAAGAAGTGATGGAGGATGGCTATAAAGGTAAAATTCTTCACTTCCTTCAAGATGCTTC
  AATTGGTGAACTTACTTTGATTCCTCAGTGTTCTCAGAAAAAGGCTCAGAAGATAACAGAACTCCGGCCC
  TTTAATAGTTGGGAGGCTCTGTTCACAAAGATGTCCAAAACTAATGGCTTATCAGAAGATTTGATATGGC
  ACTGTAAAACACTGATCCAAGAAAGAGATGTAGTTATAAGGCTTATGAACAAATGTGAAGACATTTCAAA
  TAAATTGACCAAACAAGTTACCATGCTTACTGGAAATGGAGGTGGATGGAACATAGAACAACCTTCCATT
  CTAAACCAGAGTTTGTCACTCAAGCCCTATCAGAAGGTTGGTTTGAATTGGCTGGCATTGGTACATAAAC
  ATGGACTTAATGGCATTTTGGCAGATGAAATGGGCCTAGGAAAAACTATTCAAGCCATTGCATTTCTGGC
  ATACCTCTATCAGGAGGGTAATAATGGTCCTCATTTGATCGTTGTTCCAGCTTCAACTATAGATAACTGG
  TTAAGGGAAGTTAATTTATGGTGCCCTACTTTGAAGGTCCTCTGTTACTATGGTTCTCAAGAAGAACGTA
  AACAAATTAGATTTAACATTCATAGTAGATATGAAGATTACAATGTAATTGTGACCACATATAACTGTGC
  GATCAGCAGTTCTGATGATCGTAGTCTGTTTCGACGGCTGAAACTTAATTACGCAATTTTTGATGAGGGC
  CATATGCTGAAGAATATGGGCTCCATTCGCTACCAGCACCTTATGACAATTAATGCAAATAACCGTTTGC
  TGCTCACAGGCACACCTGTACAGAACAATCTGTTAGAACTCATGTCGCTGTTGAATTTTGTTATGCCACA
  CATGTTTAGTAGTAGCACCAGTGAAATACGAAGAATGTTTTCCTCTAAGACAAAATCAGCAGATGAGCAA
  AGCATATATGAAAAGGAGAGAATAGCACATGCAAAACAAATTATAAAGCCATTTATTCTCAGAAGAGTAA
  AAGAAGAGGTTCTCAAGCAGTTACCCCCCAAGAAAGATCGAATTGAGTTGTGTGCAATGTCGGAGAAGCA
  GGAGCAACTCTATTTGGGTCTTTTCAACAGATTGAAAAAATCTATCAATAACTTGGTCACAGAAAAAAAC
  ACAGAAATGTGCAATGTCATGATGCAGTTGAGGAAAATGGCCAATCATCCTTTATTACATCGCCAATATT
  ACACAGCTGAAAAACTCAAGGAAATGTCTCAGCTTATGCTAAAGGAACCTACACATTGTGAGGCTAACCC
  TGACCTGATCTTTGAAGATATGGAAGTTATGACAGACTTCGAACTACATGTACTTTGTAAACAGTACCGA
  CACATTAATAACTTTCAGTTAGACATGGACTTGATTTTAGATTCTGGAAAATTTCGAGTTTTAGGATGCA
  TCTTGTCTGAATTGAAACAGAAGGGTGATAGAGTTGTGTTATTTAGCCAATTTACCATGATGCTGGATAT
  CTTAGAGGTTCTATTAAAACATCATCAGCATAGGTACCTCAGATTAGATGGAAAGACTCAGATTTCTGAA
  AGGATTCATCTAATTGATGAGTTTAATACCGATATGGATATCTTTGTGTTTCTGCTATCAACAAAAGCTG
  GTGGATTAGGAATAAATCTGACTTCAGCAAATGTTGTTATACTTCACGATATTGACTGTAATCCTTATAA
  TGACAAACAAGCAGAAGATAGATGCCATAGAGTAGGCCAGACTAAAGAAGCACTAGTTATAAAACTAATA
  AGCCAAGGGACGATTGAAGAATCCATGCTAAAAATTAACCAACAGAAATTGAAACTAGAACAGGATATGA
  CTACAGTAGATGAAGGTGATGAAGGGAGTATGCCAGCAGATATAGCCACATTACTAAAAACATCAATGGG
  CCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208438 representing NM_020159
  Red=Cloning site Green=Tags(s)
  
  MNLFNLDRFRFEKRNKIEEAPEATPQPSQPGPSSPISLSAEEENAEGEVSRANTPDSDITEKTEDSSVPE
  TPDNERKASISYFKNQRGIQYIDLSSDSEDVVSPNCSNTVQEKTFNKDTVIIVSEPSEDEESQGLPTMAR
  RNDDISELEDLSELEDLKDAKLQTLKELFPQRSDNDLLKLIESTSTMDGAIAAALLMFGDAGGGPRKRKL
  SSSSEPYEEDEFNDDQSIKKTRLDHGEESNESAESSNNWEKQESIVLKLQKEFPNFDKQELREVLKEHEW
  MYTEALESLKVFAEDQDMQYASQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKKRKKNVFN
  QKRVVEDSEYDSGSDVGSSLDEDYSSGEEVMEDGYKGKILHFLQDASIGELTLIPQCSQKKAQKITELRP
  FNSWEALFTKMSKTNGLSEDLIWHCKTLIQERDVVIRLMNKCEDISNKLTKQVTMLTGNGGGWNIEQPSI
  LNQSLSLKPYQKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLYQEGNNGPHLIVVPASTIDNW
  LREVNLWCPTLKVLCYYGSQEERKQIRFNIHSRYEDYNVIVTTYNCAISSSDDRSLFRRLKLNYAIFDEG
  HMLKNMGSIRYQHLMTINANNRLLLTGTPVQNNLLELMSLLNFVMPHMFSSSTSEIRRMFSSKTKSADEQ
  SIYEKERIAHAKQIIKPFILRRVKEEVLKQLPPKKDRIELCAMSEKQEQLYLGLFNRLKKSINNLVTEKN
  TEMCNVMMQLRKMANHPLLHRQYYTAEKLKEMSQLMLKEPTHCEANPDLIFEDMEVMTDFELHVLCKQYR
  HINNFQLDMDLILDSGKFRVLGCILSELKQKGDRVVLFSQFTMMLDILEVLLKHHQHRYLRLDGKTQISE
  RIHLIDEFNTDMDIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAEDRCHRVGQTKEALVIKLI
  SQGTIEESMLKINQQKLKLEQDMTTVDEGDEGSMPADIATLLKTSMGL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020159
• ORF Size: 3084 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020159.1, NP_064544.1
• RefSeq Size: 4935
• RefSeq ORF: 3081
• Locus ID: 56916
• Domains: SNF2_N, DEAD, helicase_C, CUE
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: This gene encodes a member of the SNF subfamily of helicase proteins. The encoded protein plays a critical role in the restoration of heterochromatin organization and propagation of epigenetic patterns following DNA replication by mediating histone H3/H4 deacetylation. Mutations in this gene are associated with adermatoglyphia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]