MAN2C1 (NM_006715) Human Tagged ORF Clone

CAT#: RG208517

MAN2C1 (GFP-tagged) - Human mannosidase, alpha, class 2C, member 1 (MAN2C1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAN2C1
• Synonyms: MAN6A8; MANA; MANA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208517 representing NM_006715
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCGCCGGCCTTGAAGCACTGGCGCACCACGCTGGAGCGGGTGGAGAAGTTCGTGTCGCCGC
  TCTACTTTACCGACTGTAACCTCCGCGGCAGGCTTTTTGGGGCCAGCTGCCCTGTGGCTGTGCTCTCCAG
  CTTCCTGACGCCGGAGAGACTTCCCTACCAGGAGGCAGTCCAGCGGGACTTCCGCCCCGCGCAGGTCGGC
  GACAGCTTCGGACCCACATGGTGGACCTGCTGGTTCCGGGTGGAGCTGACCATCCCAGAGGCATGGGTGG
  GCCAGGAAGTTCACCTTTGCTGGGAAAGTGATGGAGAAGGTCTGGTGTGGCGTGATGGAGAACCTGTCCA
  GGGTTTAACCAAAGAGGGTGAGAAGACCAGCTATGTCCTGACTGACAGGCTGGGGGAAAGAGACCCCCGA
  AGCCTCACTCTCTATGTGGAAGTAGCCTGCAATGGGCTCCTGGGGGCCGGGAAGGGAAGCATGATTGCAG
  CCCCTGACCCTGAGAAGATGTTCCAGCTGAGCCGGGCTGAGCTAGCTGTGTTCCACCGGGATGTCCACAT
  GCTCCTGGTGGATCTGGAGCTGCTGCTGGGCATAGCCAAGGGCCTCGGGAAGGACAACCAGCGCAGCTTC
  CAGGCCCTGTACACAGCCAATCAGATGGTGAACGTGTGTGACCCTGCCCAGCCCGAGACCTTCCCAGTGG
  CCCAGGCCCTGGCCTCCAGGTTCTTTGGCCAACATGGGGGTGAAAGCCAACACACCATTCATGCCACAGG
  GCACTGCCACATTGATACAGCCTGGCTTTGGCCCTTCAAAGAGACTGTGAGGAAATGTGCCCGGAGCTGG
  GTGACCGCCCTGCAGCTCATGGAGCGGAACCCTGAGTTCATCTTTGCCTGCTCCCAGGCGCAGCAGCTGG
  AATGGGTGAAGAGCCGCTACCCTGGCCTGTACTCCCGCATCCAGGAGTTTGCGTGCCGTGGGCAGTTTGT
  GCCTGTGGGGGGCACCTGGGTGGAGATGGATGGGAACCTGCCCAGTGGAGAGGCCATGGTGAGGCAGTTT
  TTGCAGGGCCAGAACTTCTTTCTGCAGGAGTTTGGGAAGATGTGCTCTGAGTTCTGGCTGCCGGACACCT
  TTGGCTACTCAGCACAGCTCCCCCAGATCATGCACGGCTGTGGCATCAGGCGCTTTCTCACCCAGAAATT
  GAGCTGGAATTTGGTGAACTCCTTCCCACACCATACATTTTTCTGGGAGGGCCTGGATGGCTCCCGTGTA
  CTGGTCCACTTCCCACCTGGCGACTCCTATGGGATGCAGGGCAGCGTGGAGGAGGTGCTGAAGACCGTGG
  CCAACAACCGGGACAAGGGGCGGGCCAACCACAGTGCCTTCCTCTTTGGCTTTGGGGATGGGGGTGGTGG
  CCCCACCCAGACCATGCTGGACCGCCTGAAGCGCCTGAGCAATACGGATGGGCTGCCCAGGGTGCAGCTA
  TCTTCTCCAAGACAGCTCTTCTCAGCACTGGAGAGTGACTCAGAGCAGCTGTGCACGTGGGTTGGGGAGC
  TCTTCTTGGAGCTGCACAATGGCACATACACCACCCATGCCCAGATCAAGAAGGGGAACCGGGAATGTGA
  GCGGATCCTGCACGACGTGGAGCTGCTCAGTAGCCTGGCCCTGGCCCGCAGTGCCCAGTTCCTATACCCA
  GCAGCCCAGCTGCAGCACCTCTGGAGGCTCCTTCTTCTGAACCAGTTCCATGATGTGGTGACTGGAAGCT
  GCATCCAGATGGTGGCAGAGGAAGCCATGTGCCATTATGAAGACATCCGTTCCCATGGCAATACACTGCT
  CAGCGCTGCAGCCGCAGCCCTGTGTGCTGGGGAGCCAGGTCCTGAGGGCCTCCTCATCGTCAACACACTG
  CCCTGGAAGCGGATCGAAGTGATGGCCCTGCCCAAACCGGGCGGGGCCCACAGCCTAGCCCTGGTGACAG
  TGCCCAGCATGGGCTATGCTCCTGTTCCTCCCCCCACCTCACTGCAGCCCCTGCTGCCCCAGCAGCCTGT
  GTTCGTAGTGCAAGAGACTGATGGCTCCGTGACTCTGGACAATGGCATCATCCGAGTGAAGCTGGACCCA
  ACTGGTCGCCTGACGTCCTTGGTCCTGGTGGCCTCTGGCAGGGAGGCCATTGCTGAGGGCGCCGTGGGGA
  ACCAGTTTGTGCTATTTGATGATGTCCCCTTGTACTGGGATGCATGGGACGTCATGGACTACCACCTGGA
  GACACGGAAGCCTGTGCTGGGCCAGGCAGGGACCCTGGCAGTGGGCACCGAGGGCGGCCTGCGGGGCAGC
  GCCTGGTTCTTGCTACAGATCAGCCCCAACAGTCGGCTTAGCCAGGAGGTTGTGCTGGACGTTGGCTGCC
  CCTATGTCCGCTTCCACACCGAGGTACACTGGCATGAGGCCCACAAGTTCCTGAAGGTGGAGTTCCCTGC
  TCGCGTGCGGAGTTCCCAGGCCACCTATGAGATCCAGTTTGGGCACCTGCAGCGACCTACCCACTACAAT
  ACCTCTTGGGACTGGGCTCGATTTGAGGTGTGGGCCCATCGCTGGATGGATCTGTCAGAACACGGCTTTG
  GGCTGGCCCTGCTCAACGACTGCAAGTATGGCGCGTCAGTGCGAGGCAGCATCCTCAGCCTCTCGCTCTT
  GCGGGCGCCTAAAGCCCCGGACGCTACTGCTGACACGGGGCGCCACGAGTTCACCTATGCACTGATGCCG
  CACAAGGGCTCTTTCCAGGATGCTGGCGTTATCCAAGCTGCCTACAGCCTAAACTTCCCCCTGTTGGCTC
  TGCCAGCCCCCAGCCCAGCGCCCGCCACCTCCTGGAGTGCGTTTTCCGTGTCTTCACCCGCGGTCGTATT
  GGAGACCGTCAAGCAGGCGGAGAGCAGCCCCCAGCGCCGCTCGCTGGTCCTGAGGCTGTATGAGGCCCAC
  GGCAGCCACGTGGACTGCTGGCTGCACTTGTCGCTGCCGGTTCAGGAGGCCATCCTCTGCGATCTCTTGG
  AGCGACCAGACCCTGCTGGCCACTTGACCCTTCGGGACAACCGCCTGAAGCTCACCTTTTCTCCCTTCCA
  AGTGCTGTCCCTGTTGCTCGTGCTTCAGCCTCCGCCACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208517 representing NM_006715
  Red=Cloning site Green=Tags(s)
  
  MAAAPALKHWRTTLERVEKFVSPLYFTDCNLRGRLFGASCPVAVLSSFLTPERLPYQEAVQRDFRPAQVG
  DSFGPTWWTCWFRVELTIPEAWVGQEVHLCWESDGEGLVWRDGEPVQGLTKEGEKTSYVLTDRLGERDPR
  SLTLYVEVACNGLLGAGKGSMIAAPDPEKMFQLSRAELAVFHRDVHMLLVDLELLLGIAKGLGKDNQRSF
  QALYTANQMVNVCDPAQPETFPVAQALASRFFGQHGGESQHTIHATGHCHIDTAWLWPFKETVRKCARSW
  VTALQLMERNPEFIFACSQAQQLEWVKSRYPGLYSRIQEFACRGQFVPVGGTWVEMDGNLPSGEAMVRQF
  LQGQNFFLQEFGKMCSEFWLPDTFGYSAQLPQIMHGCGIRRFLTQKLSWNLVNSFPHHTFFWEGLDGSRV
  LVHFPPGDSYGMQGSVEEVLKTVANNRDKGRANHSAFLFGFGDGGGGPTQTMLDRLKRLSNTDGLPRVQL
  SSPRQLFSALESDSEQLCTWVGELFLELHNGTYTTHAQIKKGNRECERILHDVELLSSLALARSAQFLYP
  AAQLQHLWRLLLLNQFHDVVTGSCIQMVAEEAMCHYEDIRSHGNTLLSAAAAALCAGEPGPEGLLIVNTL
  PWKRIEVMALPKPGGAHSLALVTVPSMGYAPVPPPTSLQPLLPQQPVFVVQETDGSVTLDNGIIRVKLDP
  TGRLTSLVLVASGREAIAEGAVGNQFVLFDDVPLYWDAWDVMDYHLETRKPVLGQAGTLAVGTEGGLRGS
  AWFLLQISPNSRLSQEVVLDVGCPYVRFHTEVHWHEAHKFLKVEFPARVRSSQATYEIQFGHLQRPTHYN
  TSWDWARFEVWAHRWMDLSEHGFGLALLNDCKYGASVRGSILSLSLLRAPKAPDATADTGRHEFTYALMP
  HKGSFQDAGVIQAAYSLNFPLLALPAPSPAPATSWSAFSVSSPAVVLETVKQAESSPQRRSLVLRLYEAH
  GSHVDCWLHLSLPVQEAILCDLLERPDPAGHLTLRDNRLKLTFSPFQVLSLLLVLQPPPH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006715
• ORF Size: 3120 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006715.2, NP_006706.2
• RefSeq Size: 3254 bp
• RefSeq ORF: 3123 bp
• Locus ID: 4123
• Cytogenetics: 15q24.2
• Domains: Glyco_hydro_38
• Protein Families: Druggable Genome
• Protein Pathways: Other glycan degradation
• Gene Summary: ''