ST8SIA4 (NM_005668) Human Tagged ORF Clone

CAT#: RG208764

ST8SIA4 (GFP-tagged) - Human ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (ST8SIA4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ST8SIA4
• Synonyms: PST; PST1; SIAT8D; ST8SIA-IV
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208764 representing NM_005668
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCCATTAGGAAGAGGTGGACGATCTGCACAATAAGTCTGCTCCTGATCTTTTATAAGACAAAAG
  AAATAGCAAGAACTGAGGAGCACCAGGAGACGCAACTCATCGGAGATGGTGAATTGTCTTTGAGTCGGTC
  ACTTGTCAATAGCTCTGATAAAATCATTCGAAAGGCTGGCTCTTCAATCTTCCAGCACAATGTAGAAGGT
  TGGAAAATCAATTCCTCTTTGGTCCTAGAGATAAGGAAGAACATACTTCGTTTCTTAGATGCAGAACGAG
  ATGTGTCAGTGGTCAAGAGCAGTTTTAAGCCTGGTGATGTCATACACTATGTGCTTGACAGGCGCCGGAC
  ACTAAACATTTCTCATGATCTACATAGCCTCCTACCTGAAGTTTCACCAATGAAGAATCGCAGGTTTAAG
  ACCTGTGCAGTTGTTGGAAATTCTGGCATTCTGTTAGACAGTGAATGTGGAAAGGAGATTGACAGTCACA
  ATTTTGTAATAAGGTGTAATCTAGCTCCTGTGGTGGAGTTTGCTGCAGATGTGGGAACTAAATCAGATTT
  TATTACCATGAATCCATCAGTTGTACAAAGAGCATTTGGAGGCTTTCGAAATGAGAGTGACAGAGAAAAA
  TTTGTGCATAGACTTTCCATGCTGAATGACAGTGTCCTTTGGATTCCTGCTTTCATGGTCAAAGGAGGAG
  AGAAGCACGTGGAGTGGGTTAATGCATTAATCCTTAAGAATAAACTGAAAGTGCGAACTGCCTATCCGTC
  ATTGAGACTTATTCATGCTGTCAGAGGTTACTGGCTGACCAACAAAGTTCCTATCAAAAGACCCAGCACA
  GGTCTTCTCATGTATACACTTGCCACAAGATTCTGTGATGAAATTCACCTGTATGGATTCTGGCCCTTCC
  CTAAGGATTTAAATGGAAAAGCGGTCAAATATCATTATTATGATGACTTAAAATATAGGTACTTTTCCAA
  TGCAAGCCCTCACAGAATGCCATTAGAATTCAAAACATTAAATGTGCTACATAATAGAGGAGCTCTAAAA
  CTGACAACAGGAAAGTGTGTAAAGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208764 representing NM_005668
  Red=Cloning site Green=Tags(s)
  
  MRSIRKRWTICTISLLLIFYKTKEIARTEEHQETQLIGDGELSLSRSLVNSSDKIIRKAGSSIFQHNVEG
  WKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLDRRRTLNISHDLHSLLPEVSPMKNRRFK
  TCAVVGNSGILLDSECGKEIDSHNFVIRCNLAPVVEFAADVGTKSDFITMNPSVVQRAFGGFRNESDREK
  FVHRLSMLNDSVLWIPAFMVKGGEKHVEWVNALILKNKLKVRTAYPSLRLIHAVRGYWLTNKVPIKRPST
  GLLMYTLATRFCDEIHLYGFWPFPKDLNGKAVKYHYYDDLKYRYFSNASPHRMPLEFKTLNVLHNRGALK
  LTTGKCVKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005668
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005668.3, NP_005659.1
• RefSeq Size: 6051
• RefSeq ORF: 1080
• Locus ID: 7903
• Domains: Glyco_transf_29
• Gene Summary: The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]