RCC2 (NM_018715) Human Tagged ORF Clone

CAT#: RG208811

RCC2 (GFP-tagged) - Human regulator of chromosome condensation 2 (RCC2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RCC2
• Synonyms: TD-60
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208811 representing NM_018715
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGGAAGAAGGCGGCGGCGGCGGCCTGGGAGGAGCCGAGCTCGGGCAACGGCACTGCCCGCGCCG
  GGCCCAGGAAACGCGGCGGCCCGGCGGGCAGGAAGCGCGAGCGGCCCGAGCGCTGCAGTAGCAGCAGCGG
  CGGCGGCAGCAGCGGCGACGAGGACGGCCTGGAGCTCGACGGGGCCCCCGGCGGGGGCAAGCGCGCGGCG
  CGGCCGGCGACAGCAGGCAAGGCGGGCGGCGCGGCCGTGGTCATCACCGAACCCGAGCACACCAAGGAGC
  GCGTCAAACTTGAAGGGTCAAAGTGCAAAGGGCAGCTTTTGATTTTTGGGGCAACCAACTGGGACTTGAT
  TGGTCGAAAAGAAGTGCCTAAACAGCAAGCTGCTTACCGCAATCTCGGTCAGAATTTGTGGGGGCCCCAC
  AGATATGGGTGCCTGGCGGGGGTCCGGGTGCGGACAGTGGTCTCGGGCTCGTGTGCTGCACACAGCCTCC
  TCATCACCACGGAAGGGAAGCTGTGGAGCTGGGGTCGAAATGAGAAGGGGCAGCTGGGACATGGTGACAC
  CAAGAGAGTAGAAGCCCCTAGACTCATCGAGGGTCTTAGCCACGAAGTGATTGTGTCTGCAGCATGTGGG
  CGGAACCACACCTTGGCCTTGACGGAAACGGGCTCCGTGTTTGCGTTTGGGGAAAACAAGATGGGGCAGC
  TGGGCCTTGGCAACCAGACAGACGCTGTTCCCAGCCCCGCGCAGATAATGTACAACGGCCAGCCAATTAC
  CAAAATGGCCTGTGGGGCTGAATTCAGTATGATAATGGACTGCAAAGGAAACCTCTATTCCTTTGGGTGC
  CCTGAATATGGTCAGCTGGGACACAACTCAGATGGGAAGTTCATCGCCCGGGCACAGCGGATAGAGTACG
  ACTGTGAACTAGTTCCCCGGCGAGTGGCCATCTTCATTGAGAAGACGAAAGATGGACAGATTCTGCCTGT
  ACCAAACGTGGTTGTACGAGACGTGGCCTGTGGCGCTAACCACACGCTGGTCCTGGACTCCCAGAAGCGA
  GTCTTCTCCTGGGGCTTTGGTGGCTATGGCCGGCTGGGCCACGCAGAGCAGAAGGATGAGATGGTCCCCC
  GCCTGGTGAAGCTGTTTGACTTCCCTGGGCGTGGGGCTTCCCAGATCTATGCTGGTTACACCTGCTCCTT
  TGCTGTCAGTGAAGTGGGTGGTCTGTTTTTCTGGGGGGCCACCAACACCTCCCGTGAATCTACCATGTAC
  CCAAAAGCAGTGCAGGACCTCTGCGGCTGGAGAATCCGGAGCCTGGCTTGTGGGAAGAGCAGCATCATTG
  TGGCCGCCGATGAGAGCACCATCAGCTGGGGTCCGTCACCGACCTTTGGGGAACTGGGCTACGGGGACCA
  CAAGCCCAAGTCTTCCACTGCAGCCCAGGAGGTAAAGACTCTGGATGGCATTTTCTCAGAGCAGGTCGCC
  ATGGGCTACTCACACTCCTTGGTGATAGCAAGAGATGAAAGTGAGACTGAGAAAGAGAAGATCAAGAAAC
  TGCCAGAATACAACCCCCGAACCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208811 representing NM_018715
  Red=Cloning site Green=Tags(s)
  
  MPRKKAAAAAWEEPSSGNGTARAGPRKRGGPAGRKRERPERCSSSSGGGSSGDEDGLELDGAPGGGKRAA
  RPATAGKAGGAAVVITEPEHTKERVKLEGSKCKGQLLIFGATNWDLIGRKEVPKQQAAYRNLGQNLWGPH
  RYGCLAGVRVRTVVSGSCAAHSLLITTEGKLWSWGRNEKGQLGHGDTKRVEAPRLIEGLSHEVIVSAACG
  RNHTLALTETGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMIMDCKGNLYSFGC
  PEYGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKR
  VFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMY
  PKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVA
  MGYSHSLVIARDESETEKEKIKKLPEYNPRTL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018715
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018715.3, NP_061185.1
• RefSeq Size: 4139
• RefSeq ORF: 1569
• Locus ID: 55920
• Gene Summary: The protein encoded by this gene is a guanine exchange factor that is active on RalA, a small GTPase. The encoded protein and RalA are both essential for proper kinetochore-microtubule function in early mitosis. This protein has been shown to be a biomarker for colorectal cancer. [provided by RefSeq, Oct 2016]