PPA2 (NM_006903) Human Tagged ORF Clone

CAT#: RG208860

PPA2 (GFP-tagged) - Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPA2
• Synonyms: HSPC124; SCFAI; SCFI; SID6-306
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208860 representing NM_006903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCGCTGCTGCGGCTGCTGCGCACGGGTGCCCCAGCCGCTGCGTGCCTGCGGTTGGGGACCAGTG
  CAGGGACCGGGTCGCGCCGTGCTATGGCCCTGTACCACACTGAGGAGCGCGGCCAGCCCTGCTCGCAGAA
  TTACCGCCTCTTCTTTAAGAATGTAACTGGTCACTACATTTCCCCCTTTCATGATATTCCTCTGAAGGTG
  AACTCTAAAGAGGAAAATGGCATTCCTATGAAGAAAGCACGAAATGATGAATATGAGAATCTGTTTAATA
  TGATTGTAGAAATACCTCGGTGGACAAATGCTAAAATGGAGATTGCCACCAAGGAGCCAATGAATCCCAT
  TAAACAATATGTAAAGGATGGAAAGCTACGCTATGTGGCGAATATCTTCCCTTACAAGGGTTATATATGG
  AATTATGGTACCCTCCCTCAGATTCTTTCTTGTGGAGAAGTTATTCATGTGAAGATCCTTGGAATTTTGG
  CTCTTATTGATGAAGGTGAAACAGATTGGAAATTAATTGCTATCAATGCGAATGATCCTGAAGCCTCAAA
  GTTTCATGATATTGATGATGTTAAGAAGTTCAAACCGGGTTACCTGGAAGCTACTCTTAATTGGTTTAGA
  TTATATAAGGTACCAGATGGAAAACCAGAAAACCAGTTTGCTTTTAATGGAGAATTCAAAAACAAGGCTT
  TTGCTCTTGAAGTTATTAAATCCACTCATCAATGTTGGAAAGCATTGCTTATGAAGAAGTGTAATGGAGG
  AGCTATAAATTGCACAAACGTGCAGATATCTGATAGCCCTTTCCGTTGCACTCAAGAGGAAGCAAGATCA
  TTAGTTGAATCGGTATCATCTTCACCAAATAAAGAAAGTAATGAAGAAGAGCAAGTGTGGCACTTCCTTG
  GCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208860 representing NM_006903
  Red=Cloning site Green=Tags(s)
  
  MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKV
  NSKEENGIPMKKARNDEYENLFNMIVEIPRWTNAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIW
  NYGTLPQILSCGEVIHVKILGILALIDEGETDWKLIAINANDPEASKFHDIDDVKKFKPGYLEATLNWFR
  LYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDSPFRCTQEEARS
  LVESVSSSPNKESNEEEQVWHFLGK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006903
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006903.4, NP_008834.3
• RefSeq Size: 1595
• RefSeq ORF: 918
• Locus ID: 27068
• Domains: Pyrophosphatase
• Protein Pathways: Oxidative phosphorylation
• Gene Summary: The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]