CCDC50 (NM_174908) Human Tagged ORF Clone

CAT#: RG208965

CCDC50 (GFP-tagged) - Human coiled-coil domain containing 50 (CCDC50), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CCDC50
• Synonyms: C3orf6; DFNA44; YMER
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208965 representing NM_174908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGTCAGCATCGACCAGTCCAAGCTGCCTGGAGTCAAGGAAGTATGCCGAGATTTTGCTGTCC
  TGGAGGACCACACCCTGGCTCACAGCCTGCAGGAACAAGAGATTGAGCATCATTTGGCATCGAACGTTCA
  GCGGAACCGTTTGGTCCAGCATGATCTCCAGGTGGCTAAGCAGCTCCAAGAGGAAGATCTGAAAGCGCAG
  GCCCAGCTCCAGAAGCGCTACAAAGACCTTGAACAACAAGACTGTGAAATTGCTCAGGAAATTCAGGAGA
  AGCTGGCTATTGAGGCAGAGAGACGACGCATTCAGGAGAAGAAGGATGAGGACATAGCTCGCCTTTTGCA
  AGAAAAGGAGTTACAGGAAGAGAAAAAGAGAAAGAAACACTTTCCAGAGTTCCCTGCAACCCGTGCTTAT
  GCAGATAGTTACTATTATGAAGATGGAGGAATGAAGCCAAGAGTGATGAAAGAAGCTGTATCTACTCCAT
  CACGAATGGCCCACAGGGATCAGGAATGGTATGATGCTGAAATTGCCAGAAAACTGCAAGAAGAAGAACT
  TTTGGCTACCCAGGTGGACATGAGAGCCGCTCAAGTAGCTCAAGATGAAGAAATCGCTCGACTTCTAATG
  GCTGAAGAAAAGAAAGCTTACAAAAAAGCCAAGGAGCGGGAGAAATCATCTTTGGACAAAAGAAAGCAAG
  ACCCCGAGTGGAAGCCAAAAACAGCTAAAGCAGCAAATTCCAAGTCAAAAGAGAGTGATGAACCTCACCA
  TTCTAAGAATGAAAGGCCAGCACGGCCACCACCACCTATCATGACAGATGGTGAAGATGCGGATTACACT
  CATTTTACAAACCAGCAGAGTTCCACACGGCATTTCTCAAAATCAGAGTCCTCTCATAAAGGTTTTCATT
  ACAAACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208965 representing NM_174908
  Red=Cloning site Green=Tags(s)
  
  MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQ
  AQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAY
  ADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLM
  AEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYT
  HFTNQQSSTRHFSKSESSHKGFHYKH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174908
• ORF Size: 918 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_174908.2, NP_777568.1
• RefSeq Size: 1926
• RefSeq ORF: 921
• Locus ID: 152137
• Gene Summary: This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]