NDUFA11 (NM_175614) Human Tagged ORF Clone

CAT#: RG208966

NDUFA11 (GFP-tagged) - Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 11, 14.7kDa (NDUFA11), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NDUFA11
• Synonyms: B14.7; CI-B14.7; MC1DN14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208966 representing NM_175614
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGAAGGTTTTTCGTCAGTACTGGGATATCCCCGATGGCACCGATTGCCACCGCAAAGCCTACA
  GCACCACCAGTATTGCCAGCGTCGCTGGCCTGACCGCCGCTGCCTACAGAGTCACACTCAATCCTCCGGG
  CACCTTCCTTGAAGGAGTGGCTAAGGTTGGACAATACACGTTCACTGCAGCTGCTGTCGGGGCCGTGTTT
  GGCCTCACCACCTGCATCAGCGCCCATGTCCGCGAGAAGCCCGACGACCCCCTGAACTACTTCCTCGGTG
  GCTGCGCCGGAGGCCTGACTCTGGGAGCACGCACGCACAACTACGGGATTGGCGCCGCCGCCTGCGTGTA
  CTTTGGCATAGCGGCCTCCCTGGTCAAGATGGGCCGGCTGGAGGGCTGGGAGGTGTTTGCAAAACCCAAG
  GTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208966 representing NM_175614
  Red=Cloning site Green=Tags(s)
  
  MAPKVFRQYWDIPDGTDCHRKAYSTTSIASVAGLTAAAYRVTLNPPGTFLEGVAKVGQYTFTAAAVGAVF
  GLTTCISAHVREKPDDPLNYFLGGCAGGLTLGARTHNYGIGAAACVYFGIAASLVKMGRLEGWEVFAKPK
  V
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175614
• ORF Size: 423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175614.2, NP_783313.1
• RefSeq Size: 819
• RefSeq ORF: 426
• Locus ID: 126328
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Oxidative phosphorylation
• Gene Summary: This gene encodes a subunit of the membrane-bound mitochondrial complex I. Complex I is composed of numerous subunits and functions as the NADH-ubiquinol reductase of the mitochondrial electron transport chain. Mutations in this gene are associated with severe mitochondrial complex I deficiency. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]