NPEPPS (NM_006310) Human Tagged ORF Clone

CAT#: RG209037

NPEPPS (GFP-tagged) - Human aminopeptidase puromycin sensitive (NPEPPS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NPEPPS
• Synonyms: AAP-S; MP100; PSA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209037 representing NM_006310
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGGCAGCTGCCGCCCCCTCCCTCGCTCGCCGCCTGCTCTTCCTCGGCCCTCCGCCTCCTCCCC
  TCCTCCTTCTCGTCTTCAGCCGCTCCTCTCGCCGCCGCCTCCACAGCCTGGGCCTCGCCGCGATGCCGGA
  GAAGAGGCCCTTCGAGCGGCTGCCTGCCGATGTCTCCCCCATCAACTACAGCCTTTGCCTCAAGCCCGAC
  TTGCTGGACTTCACCTTCGAGGGCAAGCTGGAGGCCGCCGCCCAGGTGAGGCAGGCGACTAATCAGATTG
  TGATGAATTGTGCTGATATTGATATTATTACAGCTTCATATGCACCAGAAGGAGATGAAGAAATACATGC
  TACAGGATTTAACTATCAGAATGAAGATGAAAAAGTCACCTTGTCTTTCCCTAGTACTCTGCAAACAGGT
  ACGGGAACCTTAAAGATAGATTTTGTTGGAGAGCTGAATGACAAAATGAAAGGTTTCTATAGAAGTAAAT
  ATACTACCCCTTCTGGAGAGGTGCGCTATGCTGCTGTAACACAGTTTGAGGCTACTGATGCCCGAAGGGC
  TTTTCCTTGCTGGGATGAGCCTGCTATCAAAGCAACTTTTGATATCTCATTGGTTGTTCCTAAAGACAGA
  GTAGCTTTATCAAACATGAATGTAATTGACCGGAAACCATACCCTGATGATGAAAATTTAGTGGAAGTGA
  AGTTTGCCCGCACACCTGTTATGTCTACATATCTGGTGGCATTTGTTGTGGGTGAATATGACTTTGTAGA
  AACAAGGTCAAAAGATGGTGTGTGTGTCCGTGTTTACACTCCTGTTGGCAAAGCAGAGCAAGGAAAATTT
  GCGTTAGAGGTTGCTGCTAAAACCTTGCCTTTTTATAAGGACTACTTCAATGTTCCTTATCCTCTACCTA
  AAATTGATCTCATTGCTATTGCAGACTTTGCAGCTGGTGCCATGGAGAACTGGGGCCTTGTTACTTATAG
  GGAGACTGCATTGCTTATTGATCCAAAAAATTCCTGTTCTTCATCCCGCCAGTGGGTTGCTCTGGTTGTG
  GGACATGAACTCGCCCATCAATGGTTTGGAAATCTTGTTACTATGGAATGGTGGACTCATCTTTGGTTAA
  ATGAAGGTTTTGCATCCTGGATTGAATATCTGTGTGTAGACCACTGCTTCCCAGAGTATGATATTTGGAC
  TCAGTTTGTTTCTGCTGATTACACCCGTGCCCAGGAGCTTGACGCCTTAGATAACAGCCATCCTATTGAA
  GTCAGTGTGGGCCATCCATCTGAGGTTGATGAGATATTTGATGCTATATCATATAGCAAAGGTGCATCTG
  TCATCCGAATGCTGCATGACTACATTGGGGATAAGGACTTTAAGAAAGGAATGAACATGTATTTAACCAA
  GTTCCAACAAAAGAATGCTGCCACAGAGGATCTCTGGGAAAGTTTAGAAAATGCTAGTGGTAAACCTATA
  GCAGCTGTGATGAATACCTGGACCAAACAAATGGGATTTCCCCTCATTTATGTGGAAGCTGAACAGGTAG
  AAGATGACAGATTATTGAGGTTGTCCCAAAAGAAGTTCTGTGCTGGTGGGTCATATGTTGGTGAAGATTG
  TCCCCAGTGGATGGTCCCTATCACAATCTCTACTAGTGAAGACCCCAACCAGGCCAAACTAAAAATTCTA
  ATGGACAAGCCAGAGATGAATGTGGTTTTGAAAAATGTCAAACCAGACCAATGGGTGAAGTTAAACTTAG
  GAACAGTTGGGTTTTATCGGACCCAGTACAGCTCTGCCATGCTGGAAAGTTTATTACCAGGCATTCGTGA
  CCTTTCTCTGCCCCCTGTGGATCGACTTGGATTACAGAATGACCTCTTCTCCTTGGCTCGAGCTGGAATC
  ATTAGCACTGTAGAGGTTCTAAAAGTCATGGAGGCTTTTGTGAATGAGCCCAATTATACTGTATGGAGCG
  ACCTGAGCTGTAACCTGGGGATTCTCTCAACTCTCTTGTCCCACACAGACTTCTATGAGGAAATCCAGGA
  GTTTGTGAAAGATGTCTTTTCACCTATAGGGGAGAGACTGGGCTGGGACCCCAAACCTGGAGAAGGTCAT
  CTCGATGCACTCCTGAGGGGCTTGGTTCTGGGAAAACTAGGAAAAGCAGGACATAAGGCAACGTTAGAAG
  AAGCCCGTCGTCGGTTTAAGGACCACGTGGAAGGAAAACAGATTCTCTCCGCTGATCTGAGGAGTCCTGT
  CTATCTGACTGTTTTGAAGCATGGTGATGGCACTACTTTAGATATTATGTTAAAACTTCATAAACAAGCA
  GATATGCAAGAAGAGAAAAACCGAATCGAAAGAGTCCTTGGCGCTACTCTTTTGCCTGACCTGATTCAAA
  AAGTCCTCACGTTTGCACTTTCAGAAGAGGTACGTCCACAGGACACTGTATCGGTAATTGGTGGAGTAGC
  TGGAGGCAGCAAGCATGGTAGGAAAGCTGCTTGGAAATTCATAAAGGACAACTGGGAAGAACTTTATAAC
  CGATACCAGGGAGGATTCTTAATATCCAGACTAATAAAGCTATCAGTTGAGGGATTTGCAGTTGATAAAA
  TGGCTGGAGAGGTTAAGGCTTTCTTCGAGAGTCACCCAGCTCCTTCAGCTGAGCGTACCATCCAGCAGTG
  TTGTGAAAATATTCTGCTGAATGCTGCCTGGCTAAAGCGAGATGCTGAGAGCATCCACCAGTACCTCCTT
  CAGCGGAAGGCCTCACCACCCACAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209037 representing NM_006310
  Red=Cloning site Green=Tags(s)
  
  MWLAAAAPSLARRLLFLGPPPPPLLLLVFSRSSRRRLHSLGLAAMPEKRPFERLPADVSPINYSLCLKPD
  LLDFTFEGKLEAAAQVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTG
  TGTLKIDFVGELNDKMKGFYRSKYTTPSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDR
  VALSNMNVIDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKF
  ALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVV
  GHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIE
  VSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLENASGKPI
  AAVMNTWTKQMGFPLIYVEAEQVEDDRLLRLSQKKFCAGGSYVGEDCPQWMVPITISTSEDPNQAKLKIL
  MDKPEMNVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGI
  ISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGH
  LDALLRGLVLGKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQA
  DMQEEKNRIERVLGATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYN
  RYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLL
  QRKASPPTV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006310
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006310.3, NP_006301.3
• RefSeq Size: 4364
• RefSeq ORF: 2760
• Locus ID: 9520
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]