PLC delta 3 (PLCD3) (NM_133373) Human Tagged ORF Clone

CAT#: RG209120

PLCD3 (GFP-tagged) - Human phospholipase C, delta 3 (PLCD3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PLCD3
• Synonyms: PLC-delta-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209120 representing NM_133373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGCGGCCGCTGGAGGCGTTGCCGCCGCCCGCCCGAGGAGCCCCCGGTGGCCGCCCAGGTCGCAG
  CCCAAGTCGCGGCGCCGGTCGCTCTCCCGTCCCCGCCGACTCCCTCCGATGGCGGCACCAAGAGGCCCGG
  GCTGCGGGCGCTGAAGAAGATGGGCCTGACGGAGGACGAGGACGTGCGCGCCATGCTGCGGGGCTCCCGG
  CTCCGCAAGATCCGCTCGCGCACGTGGCACAAGGAGCGGCTGTACCGGCTGCAGGAGGACGGCCTGAGCG
  TGTGGTTCCAGCGGCGCATCCCGCGTGCGCCATCGCAGCACATCTTCTTCGTGCAGCACATCGAGGCGGT
  CCGCGAGGGCCACCAGTCCGAGGGCCTGCGGCGCTTCGGGGGTGCCTTCGCGCCAGCGCGCTGCCTCACC
  ATCGCCTTCAAGGGCCGCCGCAAGAACCTGGACCTGGCGGCGCCCACGGCTGAGGAAGCGCAGCGCTGGG
  TGCGCGGTCTGACCAAGCTCCGCGCGCGCCTGGACGCCATGAGCCAGCGCGAGCGGCTAGACCACTGGAT
  CCACTCCTATCTGCACCGGGCTGACTCCAACCAGGACAGCAAGATGAGCTTCAAGGAGATCAAGAGCCTG
  CTGAGAATGGTCAACGTGGACATGAACGACATGTACGCCTACCTCCTCTTCAAGGAGTGTGACCACTCCA
  ACAACGACCGTCTAGAGGGGGCTGAGATCGAGGAGTTCCTGCGGCGGCTGCTGAAGCGGCCGGAGCTGGA
  GGAGATCTTCCATCAGTACTCGGGCGAGGACCGCGTGCTGAGTGCCCCTGAGCTGCTGGAGTTCCTGGAG
  GACCAGGGCGAGGAGGGCGCCACACTGGCCCGCGCCCAGCAGCTCATTCAGACCTATGAGCTCAACGAGA
  CAGCCAAGCAGCATGAGCTGATGACACTGGATGGCTTCATGATGTACCTGTTGTCGCCGGAGGGGGCTGC
  CTTGGACAACACCCACACGTGTGTGTTCCAGGACATGAACCAGCCCCTTGCCCACTACTTCATCTCTTCC
  TCCCACAACACCTATCTGACTGACTCCCAGATCGGGGGGCCCAGCAGCACCGAGGCCTATGTTAGGGCCT
  TTGCCCAGGGATGCCGCTGCGTGGAGCTGGACTGCTGGGAGGGGCCAGGAGGGGAGCCCGTCATCTATCA
  TGGCCATACCCTCACCTCCAAGATTCTCTTCCGGGACGTGGTCCAAGCCGTGCGCGACCATGCCTTCACG
  CTGTCCCCTTACCCTGTCATCCTATCCCTGGAGAACCACTGCGGGCTGGAGCAGCAGGCTGCCATGGCCC
  GCCACCTCTGCACCATCCTGGGGGACATGCTGGTGACACAGGCGCTGGACTCCCCAAATCCCGAGGAGCT
  GCCATCCCCAGAGCAGCTGAAGGGCCGGGTCCTGGTGAAGGGAAAGAAGCTGCCCGCTGCTCGGAGCGAG
  GATGGCCGGGCTCTGTCGGATCGGGAGGAGGAGGAGGAGGATGACGAGGAGGAAGAAGAGGAGGTGGAGG
  CTGCAGCGCAGAGGCGGCTGGCCAAGCAGATCTCCCCGGAGCTGTCGGCCCTGGCTGTGTACTGCCACGC
  CACCCGCCTGCGGACCCTGCACCCTGCCCCCAACGCCCCACAACCCTGCCAGGTCAGCTCCCTCAGCGAG
  CGCAAAGCCAAGAAACTCATTCGGGAGGCAGGGAACAGCTTTGTCAGGCACAATGCCCGCCAGCTGACCC
  GCGTGTACCCGCTGGGGCTGCGGATGAACTCAGCCAACTACAGTCCCCAGGAGATGTGGAACTCGGGCTG
  TCAGCTGGTGGCCTTGAACTTCCAGACGCCAGGCTACGAGATGGACCTCAATGCCGGGCGCTTCCTAGTC
  AATGGGCAGTGTGGCTACGTCCTAAAACCTGCCTGCCTGCGGCAACCTGACTCGACCTTTGACCCCGAGT
  ACCCAGGACCTCCCAGAACCACTCTCAGCATCCAGGTGCTGACTGCACAGCAGCTGCCCAAGCTGAATGC
  CGAGAAGCCACACTCCATTGTGGACCCCCTGGTGCGCATTGAGATCCATGGGGTGCCCGCAGACTGTGCC
  CGGCAGGAGACTGACTACGTGCTCAACAATGGCTTCAACCCCCGCTGGGGGCAGACCCTGCAGTTCCAGC
  TGCGGGCTCCGGAGCTGGCACTGGTCCGGTTTGTGGTGGAAGATTATGACGCCACCTCCCCCAATGACTT
  TGTGGGCCAGTTTACACTGCCTCTTAGCAGCCTAAAGCAAGGGTACCGCCACATACACCTGCTTTCCAAG
  GACGGGGCCTCACTGTCACCAGCCACGCTCTTCATCCAAATCCGCATCCAGCGCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209120 representing NM_133373
  Red=Cloning site Green=Tags(s)
  
  MLCGRWRRCRRPPEEPPVAAQVAAQVAAPVALPSPPTPSDGGTKRPGLRALKKMGLTEDEDVRAMLRGSR
  LRKIRSRTWHKERLYRLQEDGLSVWFQRRIPRAPSQHIFFVQHIEAVREGHQSEGLRRFGGAFAPARCLT
  IAFKGRRKNLDLAAPTAEEAQRWVRGLTKLRARLDAMSQRERLDHWIHSYLHRADSNQDSKMSFKEIKSL
  LRMVNVDMNDMYAYLLFKECDHSNNDRLEGAEIEEFLRRLLKRPELEEIFHQYSGEDRVLSAPELLEFLE
  DQGEEGATLARAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALDNTHTCVFQDMNQPLAHYFISS
  SHNTYLTDSQIGGPSSTEAYVRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVVQAVRDHAFT
  LSPYPVILSLENHCGLEQQAAMARHLCTILGDMLVTQALDSPNPEELPSPEQLKGRVLVKGKKLPAARSE
  DGRALSDREEEEEDDEEEEEEVEAAAQRRLAKQISPELSALAVYCHATRLRTLHPAPNAPQPCQVSSLSE
  RKAKKLIREAGNSFVRHNARQLTRVYPLGLRMNSANYSPQEMWNSGCQLVALNFQTPGYEMDLNAGRFLV
  NGQCGYVLKPACLRQPDSTFDPEYPGPPRTTLSIQVLTAQQLPKLNAEKPHSIVDPLVRIEIHGVPADCA
  RQETDYVLNNGFNPRWGQTLQFQLRAPELALVRFVVEDYDATSPNDFVGQFTLPLSSLKQGYRHIHLLSK
  DGASLSPATLFIQIRIQRS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133373
• ORF Size: 2367 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133373.3, NP_588614.1
• RefSeq Size: 3477
• RefSeq ORF: 2370
• Locus ID: 113026
• Domains: C2, PI-PLC-X, PI-PLC-Y, PH
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008]