KCNK5 (NM_003740) Human Tagged ORF Clone

CAT#: RG209207

KCNK5 (GFP-tagged) - Human potassium channel, subfamily K, member 5 (KCNK5)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNK5
• Synonyms: K2p5.1; KCNK5b; TASK-2; TASK2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209207 representing NM_003740
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACCGGGGCCCTCTGCTCACCTCGGCCATCATCTTCTACCTGGCCATCGGGGCGGCGATCTTCG
  AAGTGCTGGAGGAGCCACACTGGAAGGAGGCCAAGAAAAACTACTACACACAGAAGCTGCATCTGCTCAA
  GGAGTTCCCGTGCCTGGGTCAGGAGGGCCTGGACAAGATCCTAGAGGTGGTATCTGATGCTGCAGGACAG
  GGTGTGGCCATCACAGGGAACCAGACCTTCAACAACTGGAACTGGCCCAATGCAATGATTTTTGCAGCGA
  CCGTCATTACCACCATTGGATATGGCAATGTGGCTCCCAAGACCCCCGCCGGTCGCCTCTTCTGTGTTTT
  CTATGGTCTCTTCGGGGTGCCGCTCTGCCTGACGTGGATCAGTGCCCTGGGCAAGTTCTTCGGGGGACGT
  GCCAAGAGACTAGGGCAGTTCCTTACCAAGAGAGGTGTGAGTCTGCGGAAGGCGCAGATCACGTGCACAG
  TCATCTTCATCGTGTGGGGCGTCCTAGTCCACCTGGTGATCCCACCCTTCGTATTCATGGTGACTGAGGG
  GTGGAACTACATCGAGGGCCTCTACTACTCCTTCATCACCATCTCCACCATCGGCTTCGGTGACTTTGTG
  GCCGGTGTGAACCCCAGCGCCAACTACCACGCCCTGTACCGCTACTTCGTGGAGCTCTGGATCTACTTGG
  GGCTGGCCTGGCTGTCCCTTTTTGTCAACTGGAAGGTGAGCATGTTTGTGGAAGTCCACAAAGCCATTAA
  GAAGCGGCGGCGGCGACGGAAGGAGTCCTTTGAGAGCTCCCCACACTCCCGGAAGGCCCTGCAGGTGAAG
  GGGAGCACAGCCTCCAAGGACGTCAACATCTTCAGCTTTCTTTCCAAGAAGGAAGAGACCTACAACGACC
  TCATCAAGCAGATCGGGAAGAAGGCCATGAAGACAAGCGGGGGTGGGGAGACGGGCCCGGGCCCAGGGCT
  GGGGCCTCAAGGCGGTGGGCTCCCAGCACTGCCCCCTTCCCTGGTGCCCCTGGTAGTCTACTCCAAGAAC
  CGGGTGCCCACCTTGGAAGAGGTGTCACAGACACTGAGGAGCAAAGGCCACGTATCAAGGTCCCCAGATG
  AGGAGGCTGTGGCACGGGCCCCTGAAGACAGCTCCCCTGCCCCCGAGGTGTTCATGAACCAGCTGGACCG
  CATCAGCGAGGAATGCGAGCCATGGGACGCCCAGGACTACCACCCACTCATCTTCCAGGACGCCAGCATC
  ACCTTCGTGAACACGGAGGCTGGCCTCTCAGACGAGGAGACCTCCAAGTCCTCGCTAGAGGACAACTTGG
  CAGGGGAGGAGAGCCCCCAGCAGGGGGCTGAAGCCAAGGCGCCCCTGAACATGGGCGAGTTCCCCTCCTC
  CTCCGAGTCCACCTTCACCAGCACTGAGTCTGAGCTCTCTGTGCCTTACGAACAGCTGATGAATGAGTAC
  AACAAGGCTAACAGCCCCAAGGGCACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209207 representing NM_003740
  Red=Cloning site Green=Tags(s)
  
  MVDRGPLLTSAIIFYLAIGAAIFEVLEEPHWKEAKKNYYTQKLHLLKEFPCLGQEGLDKILEVVSDAAGQ
  GVAITGNQTFNNWNWPNAMIFAATVITTIGYGNVAPKTPAGRLFCVFYGLFGVPLCLTWISALGKFFGGR
  AKRLGQFLTKRGVSLRKAQITCTVIFIVWGVLVHLVIPPFVFMVTEGWNYIEGLYYSFITISTIGFGDFV
  AGVNPSANYHALYRYFVELWIYLGLAWLSLFVNWKVSMFVEVHKAIKKRRRRRKESFESSPHSRKALQVK
  GSTASKDVNIFSFLSKKEETYNDLIKQIGKKAMKTSGGGETGPGPGLGPQGGGLPALPPSLVPLVVYSKN
  RVPTLEEVSQTLRSKGHVSRSPDEEAVARAPEDSSPAPEVFMNQLDRISEECEPWDAQDYHPLIFQDASI
  TFVNTEAGLSDEETSKSSLEDNLAGEESPQQGAEAKAPLNMGEFPSSSESTFTSTESELSVPYEQLMNEY
  NKANSPKGT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003740
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003740.3, NP_003731.1
• RefSeq Size: 3800
• RefSeq ORF: 1500
• Locus ID: 8645
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The message for this gene is mainly expressed in the cortical distal tubules and collecting ducts of the kidney. The protein is highly sensitive to external pH and this, in combination with its expression pattern, suggests it may play an important role in renal potassium transport. [provided by RefSeq, Jul 2008]