HS3ST1 (NM_005114) Human Tagged ORF Clone

CAT#: RG209256

HS3ST1 (GFP-tagged) - Human heparan sulfate (glucosamine) 3-O-sulfotransferase 1 (HS3ST1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HS3ST1
• Synonyms: 3OST; 3OST1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209256 representing NM_005114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGCTGCTCCTGGGCGCGGTGCTGCTGGTGGCCCAGCCCCAGCTAGTGCCTTCCCGCACCGCCG
  AGCTAGGCCAGCAGGAGCTTCTGCGGAAAGCGGGGACCCTCCAGGATGACGTCCGCGATGGCGTGGCCCC
  AAACGGCTCTGCCCAGCAGTTGCCGCAGACCATCATCATCGGCGTGCGCAAGGGCGGCACGCGCGCACTG
  CTGGAGATGCTCAGCCTGCACCCCGACGTGGCGGCCGCGGAGAACGAGGTCCACTTCTTCGACTGGGAGG
  AGCATTACAGCCACGGCTTGGGCTGGTACCTCAGCCAGATGCCCTTCTCCTGGCCACACCAGCTCACAGT
  GGAGAAGACCCCCGCGTATTTCACGTCGCCCAAAGTGCCTGAGCGAGTCTACAGCATGAACCCGTCCATC
  CGGCTGCTGCTCATCCTGCGAGACCCGTCGGAGCGCGTGCTATCTGACTACACCCAAGTGTTCTACAACC
  ACATGCAGAAGCACAAGCCCTACCCGTCCATCGAGGAGTTCCTGGTGCGCGATGGCAGGCTCAATGTGGA
  CTACAAGGCCCTCAACCGCAGCCTCTACCACGTGCACATGCAGAACTGGCTGCGCTTTTTCCCGCTGCGC
  CACATCCACATTGTGGACGGCGACCGCCTCATCAGGGACCCCTTCCCTGAGATCCAAAAGGTCGAGAGGT
  TCCTAAAGCTGTCGCCGCAGATCAATGCTTCGAACTTCTACTTTAACAAAACCAAGGGCTTTTACTGCCT
  GCGGGACAGCGGCCGGGACCGCTGCTTACATGAGTCCAAAGGCCGGGCGCACCCCCAAGTCGATCCCAAA
  CTACTCAATAAACTGCACGAATATTTTCATGAGCCAAATAAGAAGTTCTTCGAGCTTGTTGGCAGAACAT
  TTGACTGGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209256 representing NM_005114
  Red=Cloning site Green=Tags(s)
  
  MAALLLGAVLLVAQPQLVPSRTAELGQQELLRKAGTLQDDVRDGVAPNGSAQQLPQTIIIGVRKGGTRAL
  LEMLSLHPDVAAAENEVHFFDWEEHYSHGLGWYLSQMPFSWPHQLTVEKTPAYFTSPKVPERVYSMNPSI
  RLLLILRDPSERVLSDYTQVFYNHMQKHKPYPSIEEFLVRDGRLNVDYKALNRSLYHVHMQNWLRFFPLR
  HIHIVDGDRLIRDPFPEIQKVERFLKLSPQINASNFYFNKTKGFYCLRDSGRDRCLHESKGRAHPQVDPK
  LLNKLHEYFHEPNKKFFELVGRTFDWH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005114
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005114.2, NP_005105.1
• RefSeq Size: 1965
• RefSeq ORF: 924
• Locus ID: 9957
• Domains: Sulfotransfer
• Protein Families: Druggable Genome
• Protein Pathways: Heparan sulfate biosynthesis
• Gene Summary: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq, Jul 2008]