TUBA3E (NM_207312) Human Tagged ORF Clone

CAT#: RG209279

TUBA3E (GFP-tagged) - Human tubulin, alpha 3e (TUBA3E)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TUBA3E
• Synonyms: tubulin, alpha 3e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209279 representing NM_207312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGAGTGTATCTCTATCCACGTGGGGCAGGCGGGTGTCCAGATCGGCAATGCCTGCTGGGAACTGT
  ACTGCCTTGAACATGGAATTCAGCCCGATGGTCAAATGCCAAGTGATAAAACCATTGGTGGCGGGGACGA
  CTCCTTCAACACGTTCTTCAGTGAGACTGGAGCTGGCAAGCACGTGCCCAGAGCAGTGTTTGTGGACCTG
  GAGCCCACTGTGGTCGATGAAGTGCGCACAGGGACCTACAGGCAGCTCTTCCACCCAGAGCAGCTGATCA
  CCGGGAAGGAAGATGCAGCCAGTAATTACGCCAGGGGCCATTACACCATCGGCAAGGAGATTGTTGACCT
  AGTCCTGGACCGGATCCGCAAACTGGCGGATCTGTGCACAGGACTGCAGGGCTTCCTCATCTTCCACAGC
  TTTGGGGGCGGCACTGGCTCTGGGTTCGCATCTCTGCTCATGGAGCGGCTCTCAGTGGATTACAGCAAGA
  AGTCCAAGCTAGAGTTTGCCATTTACCCAGCCCCCCAGGTCTCCACAGCCGTGGTGGAGCCCTACAACTC
  CATCCTAACCACCCACACGACCCTGGAACATTCTGACTGTGCCTTCATGGTCGACAATGAAGCCATCTAT
  GACATATGTCGGCGCAACCTGGACATTGAACGTCCCACGTACACCAACCTCAATCGCCTGATTGGGCAGA
  TCGTGTCCTCCATCACGGCCTCCCTGCGATTTGATGGGGCCCTGAATGTGGACTTGACGGAATTCCAGAC
  CAACCTCGTGCCGTACCCCCGCATCCACTTCCCCCTGGCCACCTACGCCCCAGTCATCTCAGCTGAGAAG
  GCCTACCATGAGCAGCTGTCTGTGGCTGAGATCACCAATGCCTGCTTCGAGCCAGCCAATCAGATGGTCA
  AGTGTGACCCTCGCCATGGCAAGTACATGGCCTGCTGCATGTTGTACAGGGGGGACGTGGTCCCCAAAGA
  CGTCAATGCGGCCATCGCCACCATCAAGACCAAGCGCACTATCCAGTTTGTGGATTGGTGCCCGACTGGA
  TTTAAGGTGGGCATTAACTACCAGCCCCCCACAGTGGTCCCCGGGGGAGACCTGGCCAAGGTGCAGCGGG
  CCGTGTGCATGCTGAGCAACACCACGGCCATTGCGGAGGCCTGGGCCCGCCTGGTCCATAAGTTCGATCT
  CATGTATGCCAAGTGGGCCTTTGTGCACTGGTACGTGGGCGAAGGCATGGAAGAGGGAGAGTTCTCTGAG
  GCCCGCGAGGACCTGGCAGCTCTAGAGAAGGATTGTGAAGAGGTGGGCGTGGATTCCGTGGAAGCTGAGG
  CTGAAGAAGGCGAAGAATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209279 representing NM_207312
  Red=Cloning site Green=Tags(s)
  
  MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
  EPTVVDEVRTGTYRQLFHPEQLITGKEDAASNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHS
  FGGGTGSGFASLLMERLSVDYSKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
  DICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
  AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTG
  FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLVHKFDLMYAKWAFVHWYVGEGMEEGEFSE
  AREDLAALEKDCEEVGVDSVEAEAEEGEEY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207312
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_207312.1, NP_997195.1
• RefSeq Size: 1554
• RefSeq ORF: 1353
• Locus ID: 112714
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection
• Gene Summary: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. This gene encodes an alpha tubulin that highly conserved among species. A missense mutation in this gene has been potentially linked to microlissencephaly and global developmental delay. [provided by RefSeq, Jul 2016]