PSG4 (NM_002780) Human Tagged ORF Clone

CAT#: RG209380

PSG4 (GFP-tagged) - Human pregnancy specific beta-1-glycoprotein 4 (PSG4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PSG4
• Synonyms: PSBG-4; PSBG-9; PSG9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209380 representing NM_002780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCCTCTCAGCCCCTCCCTGCACACACCTCATCACTTGGAAGGGGGTCCTGCTCACAGCATCAC
  TTTTAAACTTCTGGAATCCGCCCACAACTGCCCAAGTCACGATTGAAGCCCAGCCACCCAAAGTTTCTGA
  GGGGAAGGATGTTCTTCTACTTGTCCACAATTTGCCCCAGAATCTTGCTGGCTACATTTGGTACAAAGGG
  CAAATGACATACCTCTACCATTACATTACATCATATGTAGTAGACGGTCAAAGAATTATATATGGGCCTG
  CATACAGTGGAAGAGAAAGAGTATATTCCAATGCATCCCTGCTGATCCAGAATGTCACGCAGGAGGATGC
  AGGATCCTACACCTTACACATCATAAAGCGACGCGATGGGACTGGAGGAGTAACTGGACATTTCACCTTC
  ACCTTACACCTGGAGACTCCCAAGCCCTCCATCTCCAGCAGCAACTTAAATCCCAGGGAGGCCATGGAGG
  CTGTGATCTTAACCTGTGATCCTGCAACTCCAGCCGCAAGCTACCAGTGGTGGATGAATGGTCAGAGCCT
  CCCTATGACTCACAGGTTGCAGCTGTCCAAAACCAACAGGACCCTCTTTATATTTGGTGTCACAAAGTAT
  ATTGCAGGACCCTATGAATGTGAAATACGGAACCCAGTGAGTGCCAGCCGCAGTGACCCAGTCACCCTGA
  ATCTCCTCCCAAAGCTGCCCAAGCCCTACATCACAATCAACAACTTAAACCCCAGAGAGAATAAGGATGT
  CTTAACCTTCACCTGTGAACCTAAGAGTGAGAACTACACCTACATTTGGTGGCTAAATGGTCAGAGCCTC
  CCTGTCAGTCCCAGGGTAAAGCGACCCATTGAAAACAGGATCCTCATTCTACCCAATGTCACGAGAAATG
  AAACAGGACCTTATCAATGTGAAATACGGGACCGATATGGTGGCATCCGCAGTGACCCAGTCACCCTGAA
  TGTCCTCTATGGTCCAGACCTCCCCAGCATTTACCCTTCATTCACCTATTACCGTTCAGGAGAAAACCTC
  TACTTGTCCTGCTTCGCCGAGTCTAACCCACGGGCACAATATTCTTGGACAATTAATGGGAAGTTTCAGC
  TATCAGGACAAAAGCTCTCTATCCCCCAAATAACTACAAAGCATAGTGGGCTCTATGCTTGCTCTGTTCG
  TAACTCAGCCACTGGCAAGGAAAGCTCCAAATCCATCACAGTCAAAGTCTCTGACTGGATATTACCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209380 representing NM_002780
  Red=Cloning site Green=Tags(s)
  
  MGPLSAPPCTHLITWKGVLLTASLLNFWNPPTTAQVTIEAQPPKVSEGKDVLLLVHNLPQNLAGYIWYKG
  QMTYLYHYITSYVVDGQRIIYGPAYSGRERVYSNASLLIQNVTQEDAGSYTLHIIKRRDGTGGVTGHFTF
  TLHLETPKPSISSSNLNPREAMEAVILTCDPATPAASYQWWMNGQSLPMTHRLQLSKTNRTLFIFGVTKY
  IAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLTFTCEPKSENYTYIWWLNGQSL
  PVSPRVKRPIENRILILPNVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPSIYPSFTYYRSGENL
  YLSCFAESNPRAQYSWTINGKFQLSGQKLSIPQITTKHSGLYACSVRNSATGKESSKSITVKVSDWILP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002780
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002780.3, NP_002771.2
• RefSeq Size: 2059 bp
• RefSeq ORF: 1260 bp
• Locus ID: 5672
• Cytogenetics: 19q13.31
• Domains: ig, IGc2, IG
• Protein Families: Secreted Protein
• Gene Summary: 'The protein encoded by this gene is a pregnancy-specific glycoprotein (PSG), one of several encoded by a cluster of similar genes on chromosome 19. This gene is a member of the carcinoembryonic antigen (CEA) gene family and may play a role in regulation of the innate immune system. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'