Selenophosphate synthetase 1 (SEPHS1) (NM_012247) Human Tagged ORF Clone

CAT#: RG209406

SEPHS1 (GFP-tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SEPHS1
• Synonyms: SELD; SPS; SPS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209406 representing NM_012247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACGCGGGAGTCCTTTAACCCGGAAAGTTACGAATTGGACAAAAGCTTCCGGCTAACCAGATTCA
  CTGAACTGAAGGGCACAGGCTGCAAAGTGCCCCAAGATGTCCTGCAAAAATTGCTGGAATCTTTACAGGA
  GAACCACTTCCAAGAAGATGAGCAGTTTCTGGGAGCCGTTATGCCAAGGCTTGGCATTGGAATGGATACT
  TGTGTCATTCCTTTGAGGCACGGTGGGCTTTCCTTGGTTCAAACCACAGATTACATTTACCCGATCGTAG
  ACGACCCTTACATGATGGGCAGGATAGCGTGTGCCAATGTCCTCAGTGACCTCTATGCAATGGGGGTCAC
  GGAATGTGACAATATGCTGATGCTCCTTGGAGTCAGTAATAAAATGACCGACAGGGAAAGGGATAAAGTG
  ATGCCTCTGATTATCCAAGGTTTTAAAGACGCAGCTGAGGAAGCAGGAACGTCTGTAACAGGCGGCCAAA
  CAGTACTAAACCCCTGGATTGTCCTGGGAGGAGTGGCTACCACTGTCTGCCAACCCAATGAATTTATCAT
  GCCAGACAATGCAGTGCCAGGGGACGTGCTGGTGCTGACAAAACCCCTGGGGACACAGGTGGCAGTGGCT
  GTGCACCAGTGGCTGGATATCCCTGAGAAATGGAATAAGATTAAACTAGTGGTCACCCAAGAAGATGTAG
  AGCTGGCCTACCAGGAGGCGATGATGAACATGGCGAGGCTCAACAGGACAGCTGCAGGACTCATGCACAC
  GTTCAATGCCCACGCCGCCACTGACATCACGGGCTTCGGGATTTTGGGCCATGCGCAGAACCTGGCCAAG
  CAGCAGAGGAACGAGGTGTCGTTTGTAATTCACAACCTCCCGGTGCTGGCCAAGATGGCTGCGGTGAGCA
  AGGCCTGCGGAAACATGTTCGGCCTCATGCACGGGACCTGCCCGGAGACTTCAGGCGGCCTTCTGATCTG
  TTTACCACGTGAGCAAGCAGCTCGGTTCTGTGCAGAGATAAAGTCCCCCAAATATGGTGAAGGCCACCAA
  GCATGGATTATTGGGATTGTAGAGAAGGGCAACCGCACAGCCAGAATCATAGACAAACCCCGGATCATCG
  AGGTCGCACCACAAGTGGCCACTCAAAATGTGAATCCCACACCCGGGGCCACCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209406 representing NM_012247
  Red=Cloning site Green=Tags(s)
  
  MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDT
  CVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKV
  MPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVA
  VHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQNLAK
  QQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQ
  AWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012247
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012247.3, NP_036379.2
• RefSeq Size: 2612
• RefSeq ORF: 1179
• Locus ID: 22929
• Domains: AIRS
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010]