C5L2 (C5AR2) (NM_018485) Human Tagged ORF Clone

CAT#: RG209676

C5AR2 (GFP-tagged) - Human G protein-coupled receptor 77 (GPR77)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C5AR2
• Synonyms: C5L2; GPF77; GPR77
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209676 representing NM_018485
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACGATTCTGTCAGCTACGAGTATGGGGATTACAGCGACCTCTCGGACCGCCCTGTGGACTGCC
  TGGATGGCGCCTGCCTGGCCATCGACCCGCTGCGCGTGGCCCCGCTCCCACTGTATGCCGCCATCTTCCT
  GGTGGGGGTGCCGGGCAATGCCATGGTGGCCTGGGTGGCTGGGAAGGTGGCCCGCCGGAGGGTGGGTGCC
  ACCTGGTTGCTCCACCTGGCCGTGGCGGATTTGCTGTGCTGTTTGTCTCTGCCCATCCTGGCAGTGCCCA
  TTGCCCGTGGAGGCCACTGGCCGTATGGTGCAGTGGGCTGTCGGGCGCTGCCCTCCATCATCCTGCTGAC
  CATGTATGCCAGCGTCCTGCTCCTGGCAGCTCTCAGTACCGACCTCTGCTTCCTGGCTCTCGGGCCTGCC
  TGGTGGTCTACGGTTCAGCGGGCGTGCGGGGTGCAGGTGGCCTGTGGGGCAGCCTGGACACTGGCCTTGC
  TGCTCACCGTGCCCTCCGCCATCTACCGCCGGCTGCACCAGGAGCACTTCCCAGCCCGGCTGCAGTGTGT
  GGTGGACTACGGCGGCTCCTCCAGCACCGAGAATGCGGTGACTGCCATCCGGTTTCTTTTTGGCTTCCTG
  GGGCCCCTGGTGGCCGTGGTCAGCTGCCACAGTGCCCTCCTGTGCTGGGCAGCCCGACGCTGCCGGCCGC
  TGGGCACAGCCATTGTGGTGGGGTTTTTTGTCTGCTGGGCACCCTACCACCTGCTGGGGCTGGTGCTCAC
  TGTGGCGGCCCCGAACTCCGCACTCCTGGCCAGGGCCCTGCGGGCTGAACCCCTCATCGTGGGCCTTGCC
  CTCGCTCACAGCTGCCTCAATCCCATGCTCTTCCTGTATTTTGGGAGGGCTCAACTCCGCCGGTCACTGC
  CAGCTGCCTGTCACTGGGCCCTGAGGGAGTCCCAGGGCCAGGACGAAAGTGTGGACAGCAAGAAATCCAC
  CAGCCATGACCTGGTCTCGGAGATGGAGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209676 representing NM_018485
  Red=Cloning site Green=Tags(s)
  
  MGNDSVSYEYGDYSDLSDRPVDCLDGACLAIDPLRVAPLPLYAAIFLVGVPGNAMVAWVAGKVARRRVGA
  TWLLHLAVADLLCCLSLPILAVPIARGGHWPYGAVGCRALPSIILLTMYASVLLLAALSTDLCFLALGPA
  WWSTVQRACGVQVACGAAWTLALLLTVPSAIYRRLHQEHFPARLQCVVDYGGSSSTENAVTAIRFLFGFL
  GPLVAVVSCHSALLCWAARRCRPLGTAIVVGFFVCWAPYHLLGLVLTVAAPNSALLARALRAEPLIVGLA
  LAHSCLNPMLFLYFGRAQLRRSLPAACHWALRESQGQDESVDSKKSTSHDLVSEMEV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018485
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018485.1, NP_060955.1
• RefSeq Size: 1287
• RefSeq ORF: 1014
• Locus ID: 27202
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]