HGD (NM_000187) Human Tagged ORF Clone
CAT#: RG210009
- TrueORF®
HGD (GFP-tagged) - Human homogentisate 1,2-dioxygenase (HGD)
"NM_000187" in other vectors (5)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | HGD |
Synonyms | AKU; HGO |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210009 representing NM_000187
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGTTAAAGTACATTTCTGGATTTGGGAATGAGTGTTCTTCAGAGGATCCTCGCTGCCCAGGTT CCCTGCCAGAAGGACAGAATAATCCTCAGGTCTGCCCCTACAATCTCTATGCTGAGCAGCTCTCAGGATC GGCTTTCACTTGTCCACGGAGCACCAATAAGAGAAGCTGGCTGTATAGGATTCTACCTTCAGTTTCTCAC AAGCCCTTTGAATCCATTGACGAAGGCCATGTCACTCACAACTGGGATGAAGTTGATCCTGATCCTAACC AGCTTAGATGGAAACCATTTGAGATTCCAAAAGCATCTCAGAAGAAAGTAGACTTTGTGAGTGGCCTGCA TACCTTGTGTGGAGCTGGAGACATAAAGTCTAACAATGGGCTTGCTATCCACATTTTCCTCTGCAATACC TCCATGGAGAACAGATGCTTTTACAATTCAGATGGGGACTTCTTGATTGTTCCGCAGAAAGGGAACCTTC TCATTTACACCGAGTTTGGCAAGATGCTTGTACAGCCCAATGAGATCTGCGTCATTCAGAGAGGAATGCG GTTCAGCATAGATGTCTTTGAGGAGACCAGGGGCTACATCTTGGAGGTCTATGGTGTCCACTTTGAGTTA CCTGACCTTGGACCAATTGGGGCCAATGGCTTGGCCAATCCTCGTGATTTCTTGATACCCATTGCCTGGT ATGAGGATCGCCAAGTACCAGGTGGTTACACGGTCATTAATAAATACCAGGGCAAGCTGTTTGCTGCCAA ACAGGATGTCTCCCCGTTCAATGTTGTGGCCTGGCACGGGAATTATACACCCTACAAGTACAACCTGAAG AATTTCATGGTTATCAACTCAGTGGCCTTTGACCATGCAGACCCATCCATTTTCACAGTATTGACTGCTA AGTCTGTCCGCCCTGGAGTGGCCATTGCTGATTTTGTCATCTTCCCACCTCGATGGGGGGTTGCTGATAA GACCTTCAGGCCTCCTTATTACCATAGGAACTGCATGAGTGAGTTCATGGGACTCATCCGAGGTCACTAT GAGGCAAAGCAAGGTGGGTTCCTGCCAGGGGGAGGGAGTCTACACAGCACAATGACCCCCCATGGACCTG ATGCTGACTGCTTTGAGAAGGCCAGCAAGGTCAAGCTGGCACCTGAGAGGATTGCCGATGGCACCATGGC ATTTATGTTTGAATCATCTTTAAGTCTGGCGGTCACAAAGTGGGGACTCAAGGCCTCCAGGTGTTTGGAT GAGAACTACCACAAGTGCTGGGAGCCACTCAAGAGCCACTTCACTCCCAACTCCAGGAACCCAGCAGAAC CTAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210009 representing NM_000187
Red=Cloning site Green=Tags(s) MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSVSH KPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGAGDIKSNNGLAIHIFLCNT SMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVIQRGMRFSIDVFEETRGYILEVYGVHFEL PDLGPIGANGLANPRDFLIPIAWYEDRQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLK NFMVINSVAFDHADPSIFTVLTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHY EAKQGGFLPGGGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLD ENYHKCWEPLKSHFTPNSRNPAEPN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000187 |
ORF Size | 1335 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000187.2, NP_000178.2 |
RefSeq Size | 1920 bp |
RefSeq ORF | 1338 bp |
Locus ID | 3081 |
Cytogenetics | 3q13.33 |
Domains | HgmA |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways, Tyrosine metabolism |
Gene Summary | 'This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC310382 | HGD (untagged)-Human homogentisate 1,2-dioxygenase (HGD) |
USD 760.00 |
|
SC321034 | HGD (untagged)-Human homogentisate 1,2-dioxygenase (HGD) |
USD 760.00 |
|
RC210009 | HGD (Myc-DDK-tagged)-Human homogentisate 1,2-dioxygenase (HGD) |
USD 420.00 |
|
RC210009L3 | Lenti ORF clone of Human homogentisate 1,2-dioxygenase (HGD), Myc-DDK-tagged |
USD 620.00 |
|
RC210009L4 | Lenti ORF clone of Human homogentisate 1,2-dioxygenase (HGD), mGFP tagged |
USD 620.00 |
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