G2A (GPR132) (NM_013345) Human Tagged ORF Clone

CAT#: RG210284

GPR132 (GFP-tagged) - Human G protein-coupled receptor 132 (GPR132)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPR132
• Synonyms: G2A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210284 representing NM_013345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCCAATGCTACTGAAAAACGGTTACAATGGAAACGCCACCCCAGTGACCACCACTGCCCCGTGGG
  CCTCCCTGGGCCTCTCCGCCAAGACCTGCAACAACGTGTCCTTCGAAGAGAGCAGGATAGTCCTGGTCGT
  GGTGTACAGCGCGGTGTGCACGCTGGGGGTGCCGGCCAACTGCCTGACTGCGTGGCTGGCGCTGCTGCAG
  GTACTGCAGGGCAACGTGCTGGCCGTCTACCTGCTCTGCCTGGCACTCTGCGAGCTGCTGTACACAGGCA
  CGCTGCCACTCTGGGTCATCTATATCCGCAACCAGCACCGCTGGACCCTAGGCCTGCTGGCCTGCAAGGT
  GACCGCCTACATCTTCTTCTGCAACATCTACGTCAGCATCCTCTTCCTGTGCTGCATCTCCTGCGACCGC
  TTCGTGGCCGTGGTGTACGCGCTGGAGAGTCGGGGCCGCCGCCGCCGGAGGACCGCCATCCTCATCTCCG
  CCTGCATCTTCATCCTCGTCGGGATCGTTCACTACCCGGTGTTCCAGACGGAAGACAAGGAGACCTGCTT
  TGACATGCTGCAGATGGACAGCAGGATTGCCGGGTACTACTACGCCAGGTTCACCGTTGGCTTTGCCATC
  CCTCTCTCCATCATCGCCTTCACCAACCACCGGATTTTCAGGAGCATCAAGCAGAGCATGGGCTTAAGCG
  CTGCCCAGAAGGCCAAGGTGAAGCACTCGGCCATCGCGGTGGTTGTCATCTTCCTAGTCTGCTTCGCCCC
  GTACCACCTGGTTCTCCTCGTCAAAGCCGCTGCCTTTTCCTACTACAGAGGAGACAGGAACGCCATGTGC
  GGCTTGGAGGAAAGGCTGTACACAGCCTCTGTGGTGTTTCTGTGCCTGTCCACGGTGAACGGCGTGGCTG
  ACCCCATTATCTACGTGCTGGCCACGGACCATTCCCGCCAAGAAGTGTCCAGAATCCATAAGGGGTGGAA
  AGAGTGGTCCATGAAGACAGACGTCACCAGGCTCACCCACAGCAGGGACACCGAGGAGCTGCAGTCGCCC
  GTGGCCCTTGCAGACCACTACACCTTCTCCAGGCCCGTGCACCCACCAGGGTCACCATGCCCTGCAAAGA
  GGCTGATTGAGGAGTCCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210284 representing NM_013345
  Red=Cloning site Green=Tags(s)
  
  MCPMLLKNGYNGNATPVTTTAPWASLGLSAKTCNNVSFEESRIVLVVVYSAVCTLGVPANCLTAWLALLQ
  VLQGNVLAVYLLCLALCELLYTGTLPLWVIYIRNQHRWTLGLLACKVTAYIFFCNIYVSILFLCCISCDR
  FVAVVYALESRGRRRRRTAILISACIFILVGIVHYPVFQTEDKETCFDMLQMDSRIAGYYYARFTVGFAI
  PLSIIAFTNHRIFRSIKQSMGLSAAQKAKVKHSAIAVVVIFLVCFAPYHLVLLVKAAAFSYYRGDRNAMC
  GLEERLYTASVVFLCLSTVNGVADPIIYVLATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSP
  VALADHYTFSRPVHPPGSPCPAKRLIEESC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013345
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013345.2, NP_037477.1
• RefSeq Size: 3652
• RefSeq ORF: 1143
• Locus ID: 29933
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein was reported to be a receptor for lysophosphatidylcholine action, but PubMedID: 15653487 retracts this finding and instead suggests this protein to be an effector of lysophosphatidylcholine action. This protein may have proton-sensing activity and may be a receptor for oxidized free fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]