Aminopeptidase A (ENPEP) (NM_001977) Human Tagged ORF Clone

CAT#: RG210521

ENPEP (GFP-tagged) - Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ENPEP
• Synonyms: APA; CD249; gp160
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210521 representing NM_001977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTGCGGAGAGAGAGGGCTCTAAGAGATACTGCATTCAAACGAAACATGTGGCCATTCTCTGTG
  CGGTGGTGGTGGGTGTAGGATTAATAGTGGGACTTGCCGTGGGCTTGACCAGATCGTGTGACTCCAGCGG
  GGACGGCGGGCCGGGCACTGCGCCAGCTCCTTCCCACCTGCCTTCTTCCACGGCCAGCCCCTCAGGTCCT
  CCTGCCCAGGACCAGGACATCTGCCCGGCCAGTGAGGATGAGAGCGGACAGTGGAAAAACTTTCGACTGC
  CGGACTTCGTCAACCCAGTCCACTACGACCTGCACGTGAAGCCCCTGTTGGAGGAGGACACCTACACGGG
  CACCGTGAGCATCTCCATCAACCTGAGCGCTCCCACCCGGTACCTGTGGCTGCACCTCCGGGAGACCAGG
  ATCACCCGGCTCCCGGAGCTGAAGAGGCCCTCTGGGGACCAGGTGCAAGTCCGGAGGTGTTTCGAGTACA
  AAAAGCAGGAGTACGTGGTGGTCGAGGCGGAGGAAGAGCTTACCCCCAGCAGTGGAGATGGCCTGTATCT
  CCTGACCATGGAGTTCGCCGGCTGGCTGAACGGCTCCCTCGTGGGATTTTATAGAACCACCTACACGGAG
  AACGGACGAGTCAAGAGCATAGTGGCCACCGATCATGAACCAACAGATGCCAGGAAATCTTTTCCTTGTT
  TTGATGAGCCCAACAAAAAGGCAACTTATACAATATCTATCACCCATCCCAAAGAATACGGAGCACTTTC
  AAATATGCCAGTGGCGAAAGAAGAGTCAGTGGATGATAAATGGACTCGAACAACTTTTGAGAAGTCTGTC
  CCCATGAGCACGTACCTGGTGTGCTTTGCTGTACATCAATTTGACTCTGTAAAGAGAATATCAAATAGTG
  GAAAACCTCTTACAATTTATGTCCAGCCAGAGCAAAAGCACACAGCCGAATATGCTGCAAACATAACTAA
  AAGTGTGTTTGATTATTTTGAAGAATACTTTGCTATGAATTATTCTCTTCCTAAATTAGATAAAATCGCT
  ATTCCAGATTTTGGCACTGGTGCCATGGAGAACTGGGGACTCATCACGTACAGAGAAACGAACCTGCTTT
  ATGACCCTAAGGAATCAGCCTCATCAAACCAACAGAGGGTGGCCACTGTGGTTGCCCATGAACTTGTGCA
  TCAGTGGTTTGGAAATATTGTGACCATGGACTGGTGGGAAGACTTGTGGCTAAATGAAGGATTTGCTTCT
  TTCTTTGAGTTTCTGGGAGTAAACCATGCAGAAACAGACTGGCAAATGCGTGACCAAATGTTACTTGAAG
  ATGTATTACCTGTTCAAGAGGATGATTCTTTGATGTCTTCGCATCCAATTATTGTGACTGTGACAACCCC
  TGATGAAATAACATCTGTTTTTGATGGAATATCCTATAGCAAGGGATCTTCTATTTTGAGAATGCTTGAA
  GACTGGATAAAACCAGAGAATTTTCAAAAAGGATGTCAGATGTACTTGGAAAAATACCAATTCAAGAATG
  CAAAAACTTCTGATTTTTGGGCAGCACTGGAAGAGGCAAGTAGGCTACCAGTGAAAGAAGTAATGGACAC
  CTGGACCAGACAGATGGGTTATCCTGTGCTTAACGTGAACGGTGTCAAGAACATCACACAGAAACGCTTT
  TTGTTGGACCCAAGAGCTAACCCTTCTCAGCCCCCTTCAGATCTTGGTTATACATGGAATATCCCAGTTA
  AATGGACTGAAGATAATATAACAAGCAGTGTGTTATTTAATAGGTCAGAAAAAGAAGGAATCACTTTGAA
  CTCCTCTAATCCTAGTGGAAATGCTTTTCTCAAAATAAACCCAGATCATATTGGGTTTTATCGTGTAAAT
  TATGAAGTAGCAACTTGGGACTCGATAGCTACAGCGCTCTCCTTGAACCACAAGACATTTTCTTCAGCAG
  ATCGTGCAAGTCTTATTGATGATGCTTTTGCCTTGGCAAGAGCTCAACTTCTAGATTATAAGGTGGCTTT
  GAACTTGACCAAGTATCTCAAAAGGGAAGAGAATTTTTTACCATGGCAGAGAGTAATTTCAGCTGTAACC
  TACATCATTAGCATGTTTGAAGATGATAAAGAGCTATATCCTATGATTGAGGAATACTTCCAAGGTCAAG
  TGAAGCCTATTGCAGATTCTCTGGGATGGAATGATGCTGGAGACCATGTCACAAAGTTACTCCGTTCCTC
  CGTGTTAGGGTTTGCGTGCAAGATGGGAGACAGAGAAGCCTTGAACAATGCTTCCTCGTTATTTGAGCAG
  TGGCTAAATGGGACTGTAAGCCTTCCCGTAAATCTCAGGCTTCTGGTGTATCGGTATGGGATGCAGAACT
  CTGGCAATGAGATTTCATGGAACTACACTCTTGAGCAATACCAGAAAACTTCATTAGCTCAAGAAAAAGA
  AAAACTGCTGTATGGATTAGCATCAGTGAAGAACGTTACTCTTTTGTCAAGGTATTTGGATTTGCTCAAG
  GACACGAACCTTATTAAAACTCAGGATGTGTTTACAGTCATTCGATATATCTCATATAACAGCTATGGGA
  AGAACATGGCCTGGAATTGGATACAACTCAACTGGGACTATCTAGTCAACAGATATACACTCAATAACAG
  AAACCTTGGCCGAATTGTCACAATAGCAGAGCCATTCAACACTGAACTGCAACTGTGGCAGATGGAGAGC
  TTTTTTGCAAAATATCCACAAGCTGGAGCAGGAGAAAAACCTAGGGAACAAGTGCTGGAAACAGTGAAAA
  ACAATATAGAGTGGCTAAAACAACATAGAAACACCATCAGAGAATGGTTTTTTAATTTACTTGAGAGTGG
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210521 representing NM_001977
  Red=Cloning site Green=Tags(s)
  
  MNFAEREGSKRYCIQTKHVAILCAVVVGVGLIVGLAVGLTRSCDSSGDGGPGTAPAPSHLPSSTASPSGP
  PAQDQDICPASEDESGQWKNFRLPDFVNPVHYDLHVKPLLEEDTYTGTVSISINLSAPTRYLWLHLRETR
  ITRLPELKRPSGDQVQVRRCFEYKKQEYVVVEAEEELTPSSGDGLYLLTMEFAGWLNGSLVGFYRTTYTE
  NGRVKSIVATDHEPTDARKSFPCFDEPNKKATYTISITHPKEYGALSNMPVAKEESVDDKWTRTTFEKSV
  PMSTYLVCFAVHQFDSVKRISNSGKPLTIYVQPEQKHTAEYAANITKSVFDYFEEYFAMNYSLPKLDKIA
  IPDFGTGAMENWGLITYRETNLLYDPKESASSNQQRVATVVAHELVHQWFGNIVTMDWWEDLWLNEGFAS
  FFEFLGVNHAETDWQMRDQMLLEDVLPVQEDDSLMSSHPIIVTVTTPDEITSVFDGISYSKGSSILRMLE
  DWIKPENFQKGCQMYLEKYQFKNAKTSDFWAALEEASRLPVKEVMDTWTRQMGYPVLNVNGVKNITQKRF
  LLDPRANPSQPPSDLGYTWNIPVKWTEDNITSSVLFNRSEKEGITLNSSNPSGNAFLKINPDHIGFYRVN
  YEVATWDSIATALSLNHKTFSSADRASLIDDAFALARAQLLDYKVALNLTKYLKREENFLPWQRVISAVT
  YIISMFEDDKELYPMIEEYFQGQVKPIADSLGWNDAGDHVTKLLRSSVLGFACKMGDREALNNASSLFEQ
  WLNGTVSLPVNLRLLVYRYGMQNSGNEISWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLK
  DTNLIKTQDVFTVIRYISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFNTELQLWQMES
  FFAKYPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001977
• ORF Size: 2871 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001977.3, NP_001968.3
• RefSeq Size: 5004 bp
• RefSeq ORF: 2874 bp
• Locus ID: 2028
• Cytogenetics: 4q25
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Renin-angiotensin system
• Gene Summary: 'The ENPEP gene encodes glutamyl aminopeptidase, a type II integral membrane protein with an extracellular zinc-binding domain. This protein can upregulate blood pressure by cleaving the N-terminal aspartate from angiotensin II, and can regulate blood vessel formation and enhance tumorigenesis in some tissues. Along with ANPEP and DPP4, ENPEP was found to be a candidate co-receptor for the coronavirus SARS-CoV-2, which causes COVID-19. [provided by RefSeq, Apr 2020]'