BEST4 (NM_153274) Human Tagged ORF Clone

CAT#: RG211033

BEST4 (GFP-tagged) - Human bestrophin 4 (BEST4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BEST4
• Synonyms: VMD2L2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211033 representing NM_153274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTTTCATACACTCTCAAAGTGGCGGAGGCCCGCTTCGGAGGTTTCTCTGGCCTGCTTCTCCGCT
  GGAGGGGAAGCATCTACAAGCTCCTCTACAAGGAATTCCTCCTCTTTGGGGCCTTGTACGCTGTGCTTAG
  CATCACCTACCGGCTGCTGCTGACCCAGGAGCAGAGGTACGTGTATGCTCAGGTGGCCCGGTACTGCAAC
  CGCTCAGCAGACCTCATTCCCTTGTCCTTTGTATTGGGTTTCTATGTGACTCTCGTGGTGAACCGCTGGT
  GGTCCCAGTACACAAGCATCCCGCTGCCAGACCAGCTGATGTGCGTCATCTCGGCTAGCGTGCACGGCGT
  GGACCAGCGGGGCCGCCTGCTGCGCCGCACCCTCATCCGCTACGCGAACCTGGCGTCCGTGCTGGTGCTG
  CGCTCGGTCAGCACCCGCGTGCTTAAGCGCTTCCCCACCATGGAGCACGTGGTGGATGCAGGTTTCATGT
  CCCAGGAAGAGAGGAAAAAGTTTGAGAGCCTGAAATCCGACTTCAACAAGTACTGGGTCCCCTGCGTCTG
  GTTCACCAACCTGGCGGCCCAGGCCCGGAGGGACGGGCGAATACGTGACGATATCGCTCTCTGTCTACTT
  TTGGAAGAGCTGAACAAGTACCGAGCCAAGTGCAGCATGCTATTCCACTATGACTGGATCAGCATCCCCC
  TCGTCTACACCCAAGTGGTGACCATAGCCGTCTACTCTTTCTTTGCCCTCTCCCTGGTTGGCCGCCAGTT
  TGTGGAGCCAGAGGCAGGGGCTGCCAAACCTCAGAAGCTTCTGAAGCCAGGCCAGGAGCCAGCCCCAGCC
  CTGGGAGACCCGGACATGTACGTGCCTCTCACCACTCTGCTGCAGTTCTTCTTCTATGCTGGCTGGCTCA
  AGGTGGCTGAACAGATCATCAACCCATTTGGTGAGGATGATGACGACTTTGAGACAAATCAGCTCATAGA
  CCGCAACTTGCAGGTGTCCCTGCTATCCGTGGACGAAATGTACCAGAACCTTCCCCCCGCTGAGAAGGAC
  CAGTACTGGGATGAGGACCAGCCGCAGCCACCCTACACTGTGGCCACGGCGGCCGAGTCTCTGCGGCCCT
  CATTCCTGGGCTCCACCTTCAACCTGCGCATGAGCGACGACCCTGAGCAGAGCCTGCAGGTGGAGGCGTC
  CCCCGGATCTGGTCGGCCCGCGCCCGCCGCGCAGACCCCGTTGCTCGGCCGCTTCCTGGGCGTAGGGGCG
  CCCTCCCCGGCCATCAGCCTCCGGAACTTCGGCCGCGTGCGAGGCACCCCCCGCCCCCCGCATCTGCTGC
  GCTTCCGGGCGGAGGAGGGCGGCGACCCCGAGGCCGCAGCCCGCATCGAGGAGGAATCGGCGGAGTCCGG
  GGACGAGGCCCTGGAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211033 representing NM_153274
  Red=Cloning site Green=Tags(s)
  
  MTVSYTLKVAEARFGGFSGLLLRWRGSIYKLLYKEFLLFGALYAVLSITYRLLLTQEQRYVYAQVARYCN
  RSADLIPLSFVLGFYVTLVVNRWWSQYTSIPLPDQLMCVISASVHGVDQRGRLLRRTLIRYANLASVLVL
  RSVSTRVLKRFPTMEHVVDAGFMSQEERKKFESLKSDFNKYWVPCVWFTNLAAQARRDGRIRDDIALCLL
  LEELNKYRAKCSMLFHYDWISIPLVYTQVVTIAVYSFFALSLVGRQFVEPEAGAAKPQKLLKPGQEPAPA
  LGDPDMYVPLTTLLQFFFYAGWLKVAEQIINPFGEDDDDFETNQLIDRNLQVSLLSVDEMYQNLPPAEKD
  QYWDEDQPQPPYTVATAAESLRPSFLGSTFNLRMSDDPEQSLQVEASPGSGRPAPAAQTPLLGRFLGVGA
  PSPAISLRNFGRVRGTPRPPHLLRFRAEEGGDPEAAARIEEESAESGDEALEP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153274
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153274.2, NP_695006.1
• RefSeq Size: 2096
• RefSeq ORF: 1422
• Locus ID: 266675
• Protein Families: Ion Channels: Other, Transmembrane
• Gene Summary: This gene is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif. Mutation in one of the family members (bestrophin 1) is associated with vitelliform macular dystrophy. The bestrophin 4 gene is predominantly expressed in the colon. [provided by RefSeq, Jul 2008]