ALG9 (NM_001077692) Human Tagged ORF Clone

CAT#: RG211388

ALG9 (GFP-tagged) - Human asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae) (ALG9), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALG9
• Synonyms: CDG1L; DIBD1; GIKANIS; LOH11CR1J
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211388 representing NM_001077692
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTAGCCTTCTTGGTTCTCAGCACTGGCATGTTTTGCTCATCATCAGCATTCCTTCCTAGTAGCT
  TCTGTATGTACACTACGTTGATAGCCATGACTGGATGGTATATGGACAAGACTTCCATTGCTGTGCTGGG
  AGTAGCAGCTGGGGCTATCTTAGGCTGGCCATTCAGTGCAGCTCTTGGTTTACCCATTGCCTTTGATTTG
  CTGGTCATGAAACACAGGTGGAAGAGTTTCTTTCATTGGTCGCTGATGGCCCTCATACTATTTCTGGTGC
  CTGTGGTGGTCATTGACAGCTACTATTATGGGAAGTTGGTGATTGCACCACTCAACATTGTTTTGTATAA
  TGTCTTTACTCCTCATGGACCTGATCTTTATGGTACAGAACCCTGGTATTTCTATTTAATTAATGGATTT
  CTGAATTTCAATGTAGCCTTTGCTTTGGCTCTCCTAGTCCTACCACTGACTTCTCTTATGGAATACCTGC
  TGCAGAGATTTCATGTTCAGAATTTAGGCCACCCGTATTGGCTTACCTTGGCTCCAATGTATATTTGGTT
  TATAATTTTCTTCATCCAGCCTCACAAAGAGGAGAGATTTCTTTTCCCTGTGTATCCACTTATATGTCTC
  TGTGGCGCTGTGGCTCTCTCTGCACTTCAGAAATGTTACCACTTTGTGTTTCAACGATATCGCCTGGAGC
  ACTATACTGTGACATCGAATTGGCTGGCATTAGGAACTGTCTTCCTGTTTGGGCTCTTGTCATTTTCTCG
  CTCTGTGGCACTGTTCAGAGGATATCACGGGCCCCTTGATTTGTATCCAGAATTTTACCGAATTGCTACA
  GACCCAACCATCCACACTGTCCCAGAAGGCAGACCTGTGAATGTCTGTGTGGGAAAAGAGTGGTATCGAT
  TTCCCAGCAGCTTCCTTCTTCCTGACAATTGGCAGCTTCAGTTCATTCCATCAGAGTTCAGAGGTCAGTT
  ACCAAAACCTTTTGCAGAAGGACCTCTGGCCACCCGGATTGTTCCTACTGACATGAATGACCAGAATCTA
  GAAGAGCCATCCAGATATATTGATATCAGTAAATGCCATTATTTAGTGGATTTGGACACCATGAGAGAAA
  CACCCCGGGAGCCAAAATATTCATCCAATAAAGAAGAATGGATCAGCTTGGCCTATAGACCATTCCTTGA
  TGCTTCTAGATCTTCAAAGCTGCTGCGGGCATTCTATGTCCCCTTCCTGTCAGATCAGTATACAGTGTAC
  GTAAACTACACCATCCTCAAACCCCGGAAAGCAAAGCAAATCAGGAAGAAAAGTGGAGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211388 representing NM_001077692
  Red=Cloning site Green=Tags(s)
  
  MMLAFLVLSTGMFCSSSAFLPSSFCMYTTLIAMTGWYMDKTSIAVLGVAAGAILGWPFSAALGLPIAFDL
  LVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIAPLNIVLYNVFTPHGPDLYGTEPWYFYLINGF
  LNFNVAFALALLVLPLTSLMEYLLQRFHVQNLGHPYWLTLAPMYIWFIIFFIQPHKEERFLFPVYPLICL
  CGAVALSALQKCYHFVFQRYRLEHYTVTSNWLALGTVFLFGLLSFSRSVALFRGYHGPLDLYPEFYRIAT
  DPTIHTVPEGRPVNVCVGKEWYRFPSSFLLPDNWQLQFIPSEFRGQLPKPFAEGPLATRIVPTDMNDQNL
  EEPSRYIDISKCHYLVDLDTMRETPREPKYSSNKEEWISLAYRPFLDASRSSKLLRAFYVPFLSDQYTVY
  VNYTILKPRKAKQIRKKSGG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077692
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077692.1, NP_001071160.1
• RefSeq Size: 6414
• RefSeq ORF: 1323
• Locus ID: 79796
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes an alpha-1,2-mannosyltransferase enzyme that functions in lipid-linked oligosaccharide assembly. Mutations in this gene result in congenital disorder of glycosylation type Il. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]