BAI3 (ADGRB3) (NM_001704) Human Tagged ORF Clone

CAT#: RG211798

BAI3 (GFP-tagged) - Human brain-specific angiogenesis inhibitor 3 (BAI3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADGRB3
• Synonyms: BAI3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211798 representing NM_001704
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCTGTTCGTAACCTGCTGATTTATATATTTTCCACCTATCTCCTGGTTATGTTTGGATTTAATG
  CTGCCCAAGACTTCTGGTGTTCAACTTTGGTGAAGGGAGTCATTTATGGATCGTATTCTGTAAGTGAAAT
  GTTTCCTAAAAACTTTACAAACTGCACTTGGACGCTGGAAAATCCAGATCCAACCAAATATAGCATTTAC
  CTGAAATTTTCCAAAAAGGACCTTAGCTGCTCTAACTTTTCACTCCTGGCTTATCAGTTTGATCATTTTT
  CCCATGAAAAAATAAAGGATCTTTTAAGAAAGAATCATTCTATAATGCAACTCTGCAATTCCAAGAATGC
  TTTCGTTTTTCTACAGTATGATAAAAATTTTATTCAAATACGTCGAGTATTTCCAACTAATTTCCCAGGA
  TTACAGAAAAAAGGGGAAGAAGATCAGAAATCTTTTTTTGAGTTTTTGGTATTGAACAAGGTCAGCCCAA
  GCCAGTTTGGTTGCCATGTATTATGTACTTGGTTGGAGAGCTGCTTAAAATCAGAAAATGGGAGAACAGA
  ATCATGTGGGATCATGTATACAAAATGCACCTGCCCTCAGCATTTGGGAGAGTGGGGGATCGACGACCAG
  TCGCTGATTTTGTTAAATAACGTGGTGTTACCCCTGAATGAGCAGACAGAGGGCTGCCTGACCCAGGAGC
  TGCAAACCACCCAAGTCTGCAATCTTACCAGGGAGGCCAAGCGACCACCCAAAGAAGAATTTGGAATGAT
  GGGAGATCATACAATTAAAAGTCAGCGACCTCGATCTGTTCATGAAAAAAGGGTCCCTCAGGAACAAGCT
  GATGCTGCTAAATTTATGGCACAAACTGGTGAATCTGGTGTGGAAGAGTGGTCCCAGTGGAGCACATGTT
  CGGTTACTTGTGGTCAAGGGTCGCAGGTGCGAACCAGAACTTGTGTATCACCTTACGGGACACACTGCAG
  CGGCCCATTAAGAGAATCAAGGGTTTGCAATAACACTGCCCTCTGTCCAGTACACGGAGTATGGGAGGAA
  TGGTCACCATGGAGTTTATGTTCATTTACATGTGGTCGAGGCCAAAGAACAAGAACAAGGTCATGCACAC
  CTCCTCAGTATGGAGGAAGGCCGTGTGAAGGACCTGAAACACATCATAAGCCTTGTAATATTGCTCTTTG
  CCCAGTTGATGGACAGTGGCAAGAGTGGAGTTCGTGGAGCCAGTGCTCAGTAACGTGCTCGAATGGGACT
  CAGCAGAGAAGCCGGCAGTGCACTGCAGCTGCCCATGGAGGCTCCGAATGCAGAGGGCCATGGGCAGAAA
  GCAGAGAGTGCTATAACCCTGAATGTACAGCCAATGGTCAATGGAATCAGTGGGGTCATTGGAGTGGTTG
  TTCCAAGTCCTGTGATGGCGGCTGGGAAAGGCGAATAAGGACCTGTCAGGGTGCAGTGATAACAGGGCAG
  CAATGTGAAGGAACGGGCGAAGAAGTGAGAAGATGCAGTGAGCAGCGATGCCCTGCACCTTATGAAATAT
  GCCCTGAGGATTATCTGATGTCGATGGTGTGGAAAAGAACTCCAGCAGGCGACTTGGCATTCAATCAATG
  TCCCCTGAATGCCACAGGCACCACTAGCAGACGCTGCTCTCTCAGTCTTCATGGAGTGGCCTTCTGGGAA
  CAGCCGAGCTTTGCAAGATGCATATCAAATGAGTACAGACACTTGCAGCATTCAATTAAAGAGCACCTTG
  CTAAGGGGCAGCGAATGCTGGCAGGTGATGGAATGTCCCAGGTGACCAAGACACTGTTGGATTTAACTCA
  GAGAAAAAATTTCTATGCAGGCGATCTTCTGATGTCTGTGGAGATCCTGAGAAATGTGACAGACACATTT
  AAAAGGGCAAGTTACATCCCTGCATCTGATGGTGTCCAGAACTTCTTTCAAATAGTTAGCAACCTTCTAG
  ATGAAGAAAACAAGGAAAAATGGGAAGATGCACAACAGATTTATCCAGGGTCAATAGAGTTAATGCAGGT
  GATTGAAGATTTTATACACATTGTTGGAATGGGGATGATGGACTTTCAGAATTCATACTTAATGACTGGA
  AATGTAGTGGCTAGTATTCAGAAGCTTCCTGCAGCCTCTGTTCTAACAGACATCAACTTTCCAATGAAAG
  GACGGAAGGGAATGGTTGACTGGGCAAGAAACTCAGAAGATAGGGTAGTAATTCCAAAAAGCATTTTCAC
  TCCGGTGTCATCAAAAGAATTAGATGAATCATCTGTATTTGTTCTTGGCGCAGTCCTATACAAAAACTTA
  GATCTAATTTTGCCCACTTTGAGAAATTATACTGTCATTAATTCCAAAATCATCGTGGTCACAATAAGGC
  CTGAACCCAAAACAACCGATTCGTTTCTGGAGATAGAACTAGCTCATTTGGCTAATGGTACTTTGAATCC
  CTATTGTGTATTGTGGGATGACTCCAAAACGAACGAGTCTTTGGGAACGTGGTCCACCCAGGGATGTAAA
  ACTGTGCTTACCGATGCATCCCATACGAAATGCTTATGTGATCGTCTCTCTACCTTCGCCATTTTGGCTC
  AGCAACCTAGAGAAATAATCATGGAATCCTCTGGCACACCTTCAGTTACCCTAATAGTAGGCAGTGGTCT
  TTCTTGCTTGGCCTTGATTACCCTAGCAGTTGTCTATGCAGCATTATGGAGGTACATACGCTCTGAGAGA
  TCCATAATACTAATTAACTTCTGCCTGTCTATCATCTCATCCAATATCCTCATACTGGTTGGACAGACTC
  AGACACATAATAAGAGTATCTGCACAACCACCACTGCATTTTTGCACTTTTTCTTCCTGGCTTCATTCTG
  TTGGGTTTTGACTGAGGCGTGGCAATCATATATGGCTGTAACTGGAAAAATTAGGACACGGCTTATAAGA
  AAACGCTTTTTGTGCCTTGGATGGGGTTTACCAGCATTAGTAGTGGCCACATCAGTAGGCTTCACCAGAA
  CAAAAGGATATGGCACTGATCACTACTGCTGGCTCTCTCTTGAAGGAGGACTACTCTATGCTTTTGTGGG
  ACCTGCAGCCGCTGTTGTCCTGGTCAACATGGTGATTGGCATTTTGGTATTTAATAAACTTGTTTCCAGA
  GATGGAATCCTAGATAAAAAGCTCAAACACAGAGCCGGTCAGATGAGTGAGCCTCATAGCGGTTTGACGC
  TCAAATGTGCCAAGTGTGGAGTAGTTTCAACAACAGCTTTGTCAGCCACCACCGCCAGTAACGCCATGGC
  GTCTCTTTGGAGCTCCTGTGTGGTGTTGCCCCTTCTGGCTTTGACGTGGATGTCTGCGGTTCTGGCCATG
  ACAGATAAACGCTCCATATTGTTTCAAATACTTTTTGCTGTGTTTGATTCATTGCAAGGCTTTGTTATAG
  TCATGGTCCACTGCATTCTTCGGAGAGAGGTTCAGGATGCATTTAGATGCCGATTGAGAAACTGTCAGGA
  TCCCATCAATGCAGATTCTTCGAGTTCGTTTCCTAATGGGCATGCTCAAATCATGACAGACTTTGAAAAG
  GATGTAGACATTGCCTGTCGATCAGTTCTTCATAAGGATATTGGTCCTTGCCGAGCAGCCACAATAACAG
  GAACACTTTCTAGGATTTCTCTAAATGATGATGAAGAAGAAAAGGGAACAAACCCTGAAGGGCTAAGCTA
  TTCAACATTGCCTGGAAATGTCATTTCCAAAGTCATCATCCAGCAACCCACAGGTTTGCACATGCCCATG
  AGTATGAATGAGCTTAGCAATCCATGTTTGAAAAAAGAAAATAGTGAATTGCGGAGAACTGTGTACTTAT
  GTACGGATGATAATTTGAGAGGGGCTGACATGGACATAGTCCATCCTCAAGAAAGAATGATGGAAAGTGA
  CTATATTGTGATGCCCAGAAGTTCTGTAAATAACCAGCCTTCAATGAAAGAAGAAAGCAAAATGAATATT
  GGCATGGAAACCTTGCCACATGAAAGGCTATTGCACTACAAAGTAAACCCTGAATTCAATATGAATCCCC
  CTGTAATGGACCAGTTCAATATGAACTTAGAGCAACATCTCGCACCCCAGGAACATATGCAGAATTTGCC
  CTTTGAACCTCGCACAGCTGTGAAGAATTTCATGGCCTCTGAGTTGGATGATAATGCAGGACTATCAAGA
  AGTGAAACTGGATCAACGATATCAATGAGTTCTTTAGAGAGAAGAAAATCACGATATTCAGACCTTGACT
  TTGAGAAGGTCATGCATACAAGGAAGAGGCATATGGAACTATTTCAAGAACTAAATCAGAAATTTCAAAC
  TTTGGACAGATTTCGGGATATACCAAATACAAGCAGTATGGAAAACCCCGCACCAAACAAGAATCCATGG
  GACACTTTCAAAAACCCCAGTGAATACCCGCATTACACCACAATCAATGTCTTAGACACAGAGGCAAAGG
  ATGCTTTGGAACTGAGGCCAGCAGAGTGGGAGAAGTGTCTGAATTTGCCTCTGGATGTGCAAGAGGGTGA
  CTTTCAAACAGAAGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211798 representing NM_001704
  Red=Cloning site Green=Tags(s)
  
  MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLENPDPTKYSIY
  LKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVFLQYDKNFIQIRRVFPTNFPG
  LQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLKSENGRTESCGIMYTKCTCPQHLGEWGIDDQ
  SLILLNNVVLPLNEQTEGCLTQELQTTQVCNLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQA
  DAAKFMAQTGESGVEEWSQWSTCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEE
  WSPWSLCSFTCGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGT
  QQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRIRTCQGAVITGQ
  QCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLNATGTTSRRCSLSLHGVAFWE
  QPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTKTLLDLTQRKNFYAGDLLMSVEILRNVTDTF
  KRASYIPASDGVQNFFQIVSNLLDEENKEKWEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTG
  NVVASIQKLPAASVLTDINFPMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNL
  DLILPTLRNYTVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCK
  TVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYAALWRYIRSER
  SIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVLTEAWQSYMAVTGKIRTRLIR
  KRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSR
  DGILDKKLKHRAGQMSEPHSGLTLKCAKCGVVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAM
  TDKRSILFQILFAVFDSLQGFVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEK
  DVDIACRSVLHKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGLHMPM
  SMNELSNPCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNNQPSMKEESKMNI
  GMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLEQHLAPQEHMQNLPFEPRTAVKNFMASELDDNAGLSR
  SETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQELNQKFQTLDRFRDIPNTSSMENPAPNKNPW
  DTFKNPSEYPHYTTINVLDTEAKDALELRPAEWEKCLNLPLDVQEGDFQTEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001704
• ORF Size: 4566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001704.1, NP_001695.1
• RefSeq Size: 5218 bp
• RefSeq ORF: 4569 bp
• Locus ID: 577
• Cytogenetics: 6q12-q13
• Domains: GPS, 7tm_2, tsp_1, HormR
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: 'This p53-target gene encodes a brain-specific angiogenesis inhibitor, a seven-span transmembrane protein, and is thought to be a member of the secretin receptor family. Brain-specific angiogenesis proteins BAI2 and BAI3 are similar to BAI1 in structure, have similar tissue specificities, and may also play a role in angiogenesis. [provided by RefSeq, Jul 2008]'