AP1B1 (NM_001127) Human Tagged ORF Clone

CAT#: RG211920

AP1B1 (GFP-tagged) - Human adaptor-related protein complex 1, beta 1 subunit (AP1B1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AP1B1
• Synonyms: ADTB1; AP105A; BAM22; CLAPB2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211920 representing NM_001127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACTCAAAATATTTCACCACGACCAAGAAAGGGGAGATCTTCGAGCTGAAGGCAGAGCTCAACA
  GTGACAAGAAGGAGAAGAAGAAGGAGGCAGTGAAGAAAGTGATTGCATCGATGACCGTGGGCAAAGATGT
  CAGTGCCCTCTTCCCCGATGTGGTCAACTGCATGCAGACGGACAACCTGGAGCTGAAGAAGCTAGTATAC
  CTCTACTTGATGAATTACGCCAAGAGTCAGCCTGACATGGCCATTATGGCCGTCAACACCTTTGTGAAGG
  ACTGTGAGGACCCCAACCCCCTCATCCGAGCCCTGGCAGTGCGGACCATGGGCTGCATCCGCGTTGACAA
  GATCACAGAGTACCTGTGCGAGCCACTCCGGAAGTGCCTGAAGGACGAGGATCCATATGTGCGCAAGACA
  GCAGCTGTGTGCGTGGCCAAGCTCCACGACATCAACGCCCAGCTGGTGGAGGACCAGGGCTTCCTGGACA
  CCCTTAAAGACCTCATCTCCGACTCTAACCCCATGGTGGTGGCCAATGCAGTGGCAGCGCTCTCAGAAAT
  TGCCGAGTCTCACCCCAGCAGCAACCTGCTCGATCTGAACCCACAGTCCATCAACAAGCTGCTGACAGCC
  CTCAATGAGTGCACCGAGTGGGGCCAGATCTTCATCCTGGACTGCCTCGCCAACTATATGCCCAAGGACG
  ACCGCGAGGCCCAGAGCATCTGTGAGCGGGTCACCCCCAGGCTCTCCCATGCCAACTCCGCTGTGGTGCT
  CTCTGCTGTGAAGGTGCTGATGAAGTTCATGGAGATGTTGTCTAAGGACTTGGACTACTACGGCACACTG
  CTCAAGAAGCTGGCCCCACCCCTGGTCACACTGCTGTCAGCCGAGCCAGAGCTGCAGTATGTGGCCCTGC
  GCAACATCAATCTCATCGTGCAGAAAAGGCCTGAGATCCTGAAGCATGAGATGAAGGTGTTCTTCGTGAA
  GTACAACGACCCTATCTACGTGAAGCTGGAGAAGCTGGACATCATGATCCGCCTGGCCTCTCAGGCCAAC
  ATCGCCCAGGTGTTGGCAGAGCTGAAAGAGTACGCAACAGAAGTGGATGTGGACTTTGTACGGAAGGCTG
  TGCGTGCTATTGGCCGCTGCGCCATCAAGGTGGAGCAATCTGCGGAGCGCTGTGTGAGCACGCTGCTCGA
  CCTCATCCAGACCAAGGTCAACTATGTGGTCCAGGAGGCCATCGTGGTCATCAAGGACATCTTCCGCAAG
  TACCCCAACAAGTATGAGAGTGTGATTGCCACACTGTGTGAGAATCTGGACTCCCTGGATGAGCCTGAGG
  CCCGGGCTGCCATGATCTGGATTGTGGGCGAGTACGCGGAACGGATCGACAACGCAGATGAGCTGCTGGA
  GAGCTTCCTCGAGGGCTTCCATGACGAGAGCACACAGGTCCAGCTGCAGCTGCTGACAGCCATTGTGAAA
  CTCTTTCTAAAGAAGCCAACAGAGACCCAGGAGCTGGTGCAGCAGGTCCTCAGTTTGGCCACTCAGGACT
  CAGATAACCCAGACCTGCGGGACCGTGGCTACATCTACTGGCGCCTGCTGTCCACGGACCCGGTGGCAGC
  CAAGGAGGTGGTGTTGGCTGAGAAGCCACTCATCTCTGAAGAGACGGACCTCATCGAGCCCACACTGTTA
  GACGAGCTTATCTGCTACATCGGCACGCTGGCTTCCGTCTACCATAAGCCTCCCAGTGCCTTTGTGGAGG
  GGGGCCGGGGCGTCGTGCACAAGAGCCTGCCACCTCGCACGGCCTCGAGTGAGAGCGCAGAGAGCCCTGA
  GACAGCCCCTACTGGAGCACCTCCTGGGGAGCAGCCAGATGTCATCCCCGCCCAGGGCGACCTGCTGGGT
  GACCTCCTCAACCTGGACCTCGGCCCCCCAGTGAGCGGCCCACCCCTGGCCACCTCCTCGGTGCAGATGG
  GAGCTGTGGACCTTCTTGGCGGTGGCCTTGACAGCCTGATGGGGGATGAGCCTGAAGGGATTGGGGGCAC
  CAACTTCGTGGCACCTCCAACAGCAGCAGTACCAGCCAATCTTGGAGCACCCATCGGCAGTGGCCTGAGT
  GACCTCTTTGACCTGACCAGTGGCGTGGGCACCCTGTCAGGATCATATGTGGCCCCCAAAGCAGTCTGGC
  TCCCAGCCATGAAGGCTAAGGGGCTGGAGATCTCAGGCACCTTCACCCGCCAGGTGGGCTCCATCTCCAT
  GGACCTGCAGCTGACCAACAAGGCCTTGCAGGTCATGACCGACTTTGCCATCCAGTTCAACCGCAACAGC
  TTTGGCCTGGCCCCCGCCACCCCCCTCCAGGTCCACGCGCCACTCAGCCCCAACCAGACAGTGGAGATCT
  CCCTGCCTCTCAGCACGGTGGGCTCGGTCATGAAGATGGAGCCTCTGAACAACCTCCAGGTGGCCGTGAA
  GAACAACATCGATGTCTTCTACTTCAGCACCTTGTACCCACTGCACATCCTCTTTGTGGAGGACGGGAAG
  ATGGACCGGCAGATGTTCCTGGCCACATGGAAGGATATTCCCAATGAGAATGAGGCCCAGTTCCAGATCA
  GAGACTGCCCCCTCAATGCAGAGGCTGCGAGCAGCAAGCTGCAGAGCAGCAACATCTTCACTGTCGCCAA
  GAGGAACGTGGAGGGCCAGGACATGCTCTACCAGTCCCTGAAGCTGACCAACGGCATCTGGGTGCTGGCG
  GAGCTGCGGATCCAGCCGGGCAACCCCAGCTGCACGGACTTAGAGCTGTCCCTGAAGTGTCGAGCACCAG
  AGGTGTCCCAGCACGTGTACCAGGCCTACGAGACCATCCTCAAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211920 representing NM_001127
  Red=Cloning site Green=Tags(s)
  
  MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVY
  LYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT
  AAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTA
  LNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYGTL
  LKKLAPPLVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQAN
  IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRK
  YPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVK
  LFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLL
  DELICYIGTLASVYHKPPSAFVEGGRGVVHKSLPPRTASSESAESPETAPTGAPPGEQPDVIPAQGDLLG
  DLLNLDLGPPVSGPPLATSSVQMGAVDLLGGGLDSLMGDEPEGIGGTNFVAPPTAAVPANLGAPIGSGLS
  DLFDLTSGVGTLSGSYVAPKAVWLPAMKAKGLEISGTFTRQVGSISMDLQLTNKALQVMTDFAIQFNRNS
  FGLAPATPLQVHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGK
  MDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLA
  ELRIQPGNPSCTDLELSLKCRAPEVSQHVYQAYETILKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127
• ORF Size: 2847 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127.2
• RefSeq Size: 4175 bp
• RefSeq ORF: 2850 bp
• Locus ID: 162
• Cytogenetics: 22q12.2
• Domains: Adaptin_N, Alpha_adaptinC2
• Protein Pathways: Lysosome
• Gene Summary: 'Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'