RIOK3 (NM_003831) Human Tagged ORF Clone

CAT#: RG212139

RIOK3 (GFP-tagged) - Human RIO kinase 3 (yeast) (RIOK3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RIOK3
• Synonyms: SUDD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212139 representing NM_003831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTGGTAGGAGTGGCATCGCCTGAGCCCGGGACGGCAGCGGCCTGGGGACCCAGCAAGTGTCCAT
  GGGCTATTCCTCAAAATACAATATCTTGTTCTTTGGCTGATGTAATGAGTGAACAGCTGGCCAAAGAATT
  GCAGTTAGAAGAAGAAGCTGCCGTTTTTCCTGAAGTTGCTGTTGCTGAAGGACCATTTATTACTGGAGAA
  AACATTGATACTTCCAGTGACCTTATGCTGGCTCAGATGCTACAGATGGAATATGACAGAGAATATGATG
  CACAGCTTAGGCGTGAAGAAAAAAAATTCAATGGAGATAGCAAAGTTTCCATTTCCTTTGAAAATTATCG
  AAAAGTGCATCCTTATGAAGACAGCGATAGCTCTGAAGATGAGGTTGACTGGCAGGATACTCGTGATGAT
  CCCTACAGACCAGCAAAACCGGTTCCCACTCCTAAAAAGGGCTTTATTGGAAAAGGAAAAGATATCACCA
  CCAAACATGATGAAGTAGTATGTGGGAGAAAGAACACAGCAAGAATGGAAAATTTTGCACCTGAGTTTCA
  GGTAGGAGATGGAATTGGAATGGATTTAAAACTATCAAACCATGTTTTCAATGCTTTAAAACAACATGCC
  TACTCAGAAGAACGTCGAAGTGCCCGCCTACATGAGAAAAAGGAGCATTCTACAGCAGAAAAAGCAGTTG
  ATCCTAAGACACGTTTACTTATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTAT
  TAGTACAGGAAAGGAGTCTGTTGTCTTTCATGCATATGGAGGGAGCATGGAGGATGAAAAGGAAGATAGT
  AAAGTTATACCTACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAATTTAAGAATCGTGACA
  AATATATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAATCCACGTAAGATCATCCGCAT
  GTGGGCAGAAAAAGAAATGCACAATCTCGCAAGAATGCAGAGAGCTGGAATTCCTTGTCCAACAGTTGTA
  CTACTGAAGAAACACATTTTAGTTATGTCTTTTATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAG
  AAGTAAAGCTCAATAGTGAAGAAATGAAAGAAGCCTACTATCAAACTCTTCATTTGATGCGGCAGTTATA
  TCATGAATGTACGCTTGTCCATGCTGACCTCAGTGAGTATAACATGCTGTGGCATGCTGGAAAGGTCTGG
  TTGATCGATGTCAGTCAGTCAGTAGAACCTACCCACCCTCACGGCCTGGAGTTCTTGTTCCGGGACTGCA
  GGAATGTCTCGCAGTTTTTCCAGAAAGGAGGAGTCAAGGAAGCCCTTAGTGAACGAGAACTCTTCAATGC
  TGTTTCAGGCTTAAACATCACAGCAGATAATGAAGCTGATTTTTTAGCTGAGATAGAAGCTTTGGAGAAA
  ATGAATGAAGATCACGTTCAGAAGAATGGAAGGAAAGCTGCTTCATTTTTGAAAGATGATGGAGACCCAC
  CACTACTATATGATGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212139 representing NM_003831
  Red=Cloning site Green=Tags(s)
  
  MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGE
  NIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDD
  PYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHA
  YSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDS
  KVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVV
  LLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVW
  LIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEK
  MNEDHVQKNGRKAASFLKDDGDPPLLYDE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003831
• ORF Size: 1557 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003831.2, NP_003822.2
• RefSeq Size: 3497
• RefSeq ORF: 1560
• Locus ID: 8780
• Domains: RIO
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2017]