SMARCA6 (HELLS) (NM_018063) Human Tagged ORF Clone

CAT#: RG212231

HELLS (GFP-tagged) - Human helicase, lymphoid-specific (HELLS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HELLS
• Synonyms: ICF4; LSH; Nbla10143; PASG; SMARCA6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212231 representing NM_018063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCGGAACGGCCCGCGGGCAGCGGCGGCTCGGAGGCTCCAGCAATGGTTGAACAACTGGACACTG
  CTGTGATTACCCCGGCCATGCTAGAAGAGGAAGAACAGCTTGAAGCTGCTGGACTAGAGAGAGAGCGGAA
  GATGCTGGAAAAGGCTCGCATGTCTTGGGATAGAGAGTCGACAGAAATTCGGTACCGTAGACTTCAACAT
  TTGCTTGAAAAAAGCAATATATACTCCAAATTTTTATTGACGAAAATGGAACAGCAACAATTAGAGGAAC
  AGAAGAAGAAAGAAAAATTGGAGAGAAAAAAGGAGTCTTTAAAAGTTAAAAAGGGTAAAAATTCAATTGA
  TGCAAGTGAAGAGAAGCCAGTTATGAGGAAAAAAAGAGGAAGAGAAGATGAATCATACAATATTTCAGAG
  GTCATGTCAAAAGAGGAAATTTTGTCTGTGGCTAAAAAAAATAAAAAGGAGAATGAGGATGAAAACTCCT
  CCTCTACTAATCTCTGTGTGGAAGATCTTCAGAAAAATAAAGATTCGAATAGTATAATTAAAGATAGATT
  GTCTGAAACGGTTAGGCAGAATACTAAATTCTTTTTTGACCCAGTCCGGAAGTGTAATGGTCAGCCAGTA
  CCTTTTCAACAACCAAAGCACTTCACTGGAGGAGTGATGCGATGGTACCAAGTAGAAGGCATGGAATGGC
  TTAGGATGCTTTGGGAAAATGGAATTAATGGCATTTTAGCAGATGAAATGGGATTGGGTAAGACAGTTCA
  GTGCATTGCTACTATTGCATTGATGATTCAGAGAGGAGTACCAGGACCTTTTCTTGTCTGTGGCCCTTTG
  TCTACACTTCCTAACTGGATGGCTGAATTCAAAAGATTTACACCAGATATCCCTACAATGTTATATCATG
  GAACCCAGGAGGAACGTCAAAAATTGGTAAGAAATATTTACAAACGGAAAGGGACTTTGCAGATTCATCC
  TGTGGTAATCACGTCATTTGAAATAGCCATGAGAGACCGAAATGCGTTACAGCATTGCTATTGGAAATAC
  TTAATAGTAGATGAAGGACACAGGATTAAGAATATGAAGTGCCGTCTAATCAGGGAGTTAAAACGATTCA
  ATGCTGATAACAAACTTCTTTTGACTGGTACTCCCTTGCAAAACAATTTATCAGAACTTTGGTCATTGCT
  AAACTTTTTGTTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTCTTGGTTTGACATCACTAGTCTT
  TCTGAAACTGCTGAAGATATTATTGCTAAAGAAAGAGAACAGAATGTATTGCATATGCTGCACCAGATTT
  TAACACCTTTCTTATTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAGTTCCTCCTAAACGAGAAGTAGT
  CGTTTATGCTCCACTTTCAAAGAAGCAGGAGATCTTTTATACAGCCATTGTGAACCGTACAATTGCAAAC
  ATGTTTGGATCCAGTGAGAAAGAAACAATTGAGTTAAGTCCTACTGGTCGACCAAAACGACGAACTAGAA
  AATCAATAAATTACAGCAAAATAGATGATTTCCCTAATGAATTGGAAAAACTGATCAGTCAAATACAGCC
  AGAGGTGGACCGAGAAAGAGCTGTTGTGGAAGTGAATATCCCTGTAGAATCTGAAGTTAATCTGAAGCTG
  CAGAATATAATGATGCTACTTCGTAAATGTTGTAATCATCCATATTTGATTGAATATCCTATAGACCCTG
  TTACACAAGAATTTAAGATCGATGAAGAATTGGTAACAAATTCTGGGAAGTTCTTGATTTTGGATCGAAT
  GCTGCCAGAACTAAAAAAAAGAGGTCACAAGGTGCTGCTTTTTTCACAAATGACAAGCATGTTGGACATT
  TTGATGGATTACTGCCATCTCAGAGATTTCAACTTCAGCAGGCTTGATGGGTCCATGTCTTACTCAGAGA
  GAGAAAAAAACATGCACAGCTTCAACACGGATCCAGAGGTGTTTATCTTCTTAGTGAGTACACGAGCTGG
  TGGCCTGGGCATTAATCTGACTGCAGCAGATACAGTTATCATTTATGATAGTGATTGGAACCCCCAGTCG
  GATCTTCAGGCCCAGGATAGATGTCATAGAATTGGTCAGACAAAGCCAGTTGTTGTTTATCGCCTTGTTA
  CAGCAAATACTATCGATCAGAAAATTGTGGAAAGAGCAGCTGCTAAAAGGAAACTGGAAAAGTTGATCAT
  CCATAAAAATCATTTCAAAGGTGGTCAGTCTGGATTAAATCTGTCTAAGAATTTCTTAGATCCTAAGGAA
  TTAATGGAATTATTAAAATCTAGAGATTATGAAAGGGAAATAAAAGGATCAAGAGAGAAGGTCATTAGTG
  ATAAAGATCTAGAGTTGTTGTTAGATCGAAGTGATCTTATTGATCAAATGAATGCTTCAGGACCAATTAA
  AGAGAAGATGGGGATATTCAAGATATTAGAAAATTCTGAAGATTCCAGTCCTGAATGTTTGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212231 representing NM_018063
  Red=Cloning site Green=Tags(s)
  
  MPAERPAGSGGSEAPAMVEQLDTAVITPAMLEEEEQLEAAGLERERKMLEKARMSWDRESTEIRYRRLQH
  LLEKSNIYSKFLLTKMEQQQLEEQKKKEKLERKKESLKVKKGKNSIDASEEKPVMRKKRGREDESYNISE
  VMSKEEILSVAKKNKKENEDENSSSTNLCVEDLQKNKDSNSIIKDRLSETVRQNTKFFFDPVRKCNGQPV
  PFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPL
  STLPNWMAEFKRFTPDIPTMLYHGTQEERQKLVRNIYKRKGTLQIHPVVITSFEIAMRDRNALQHCYWKY
  LIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSL
  SETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFYTAIVNRTIAN
  MFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKL
  QNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDI
  LMDYCHLRDFNFSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQS
  DLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKE
  LMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018063
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018063.3, NP_060533.2
• RefSeq Size: 3165 bp
• RefSeq ORF: 2517 bp
• Locus ID: 3070
• Cytogenetics: 10q23.33
• Domains: SNF2_N, DEAD, helicase_C
• Gene Summary: 'This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]'