UBE2L6 (NM_004223) Human Tagged ORF Clone

CAT#: RG212405

UBE2L6 (GFP-tagged) - Human ubiquitin-conjugating enzyme E2L 6 (UBE2L6), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UBE2L6
• Synonyms: RIG-B; UBCH8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212405 representing NM_004223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCGAGCATGCGAGTGGTGAAGGAGCTGGAGGATCTTCAGAAGAAGCCTCCCCCATACCTGCGGA
  ACCTGTCCAGCGATGATGCCAATGTCCTGGTGTGGCACGCTCTCCTCCTACCCGACCAACCTCCCTACCA
  CCTGAAAGCCTTCAACCTGCGCATCAGCTTCCCGCCGGAGTATCCGTTCAAGCCTCCCATGATCAAATTC
  ACAACCAAGATCTACCACCCCAACGTGGACGAGAACGGACAGATTTGCCTGCCCATCATCAGCAGTGAGA
  ACTGGAAGCCTTGCACCAAGACTTGCCAAGTCCTGGAGGCCCTCAATGTGCTGGTGAATAGACCGAATAT
  CAGGGAGCCCCTGCGGATGGACCTCGCTGACCTGCTGACACAGAATCCGGAGCTGTTCAGAAAGAATGCC
  GAAGAGTTCACCCTCCGATTCGGAGTGGACCGGCCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212405 representing NM_004223
  Red=Cloning site Green=Tags(s)
  
  MMASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKF
  TTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNA
  EEFTLRFGVDRPS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004223
• ORF Size: 459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004223.3, NP_004214.1
• RefSeq Size: 1260
• RefSeq ORF: 462
• Locus ID: 9246
• Domains: UBCc
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011]