CRMP1 (NM_001014809) Human Tagged ORF Clone

CAT#: RG212919

CRMP1 (GFP-tagged) - Human collapsin response mediator protein 1 (CRMP1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CRMP1
• Synonyms: CRMP-1; DPYSL1; DRP-1; DRP1; ULIP-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212919 representing NM_001014809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCGGCGGCGCGCGTGGAACACCGAGGACGACCTGCCCGTGTACCTGGCGCGGCCGGGCAGCG
  CGGCGCAGACCCCGCGCCAGAAGTACGGCGGCATGTTCGCCGCGGTGGAGGGCGCCTACGAGAACAAGAC
  CATCGACTTCGACGCCTACAGTGTGGGCCGCCGCGGCTCGGCGCGCACGCCGCGCAGCGCTGGCCGGCCC
  GACGCGGTCGGGCTGCCAGGGCCGGGAGGCAGCGAGGACACGGCCAGCGACGTGAGCGAGCCCTCGGGCT
  CCGCGGTCAGCTCTCCCGGAGAGCGCGACGAGCGGCCGCCGACGCTGCGCATCCGCCGCCCCGCGCCCCG
  CGACCTGCCCCTCGGCCGGGACAATGGCCAGAGTGACCGACTCCTCATCAAAGGTGGACGGATCATCAAC
  GATGACCAATCCCTTTATGCTGACGTCTACCTGGAGGATGGACTTATCAAACAAATAGGAGAGAACTTAA
  TCGTTCCTGGTGGAGTGAAGACCATTGAAGCCAACGGGCGGATGGTTATTCCCGGAGGTATTGATGTCAA
  CACGTACCTGCAGAAGCCCTCCCAGGGGATGACTGCGGCTGATGACTTCTTCCAAGGGACCAGGGCGGCA
  CTGGTGGGCGGGACCACGATGATCATTGACCATGTTGTTCCTGAACCTGGGTCCAGCCTACTGACCTCTT
  TCGAGAAGTGGCACGAAGCAGCTGACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGACATCACAAG
  CTGGTACGATGGCGTTCGGGAGGAGCTGGAGGTGCTGGTGCAGGACAAAGGCGTCAATTCCTTCCAAGTC
  TACATGGCCTATAAGGATGTCTACCAAATGTCCGACAGCCAGCTCTATGAAGCCTTTACCTTCCTTAAGG
  GCCTGGGAGCTGTGATCTTGGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGAACAAAAGCGGATCCT
  GGAGATGGGCATCACGGGTCCCGAGGGCCATGCCCTGAGCAGACCTGAAGAGCTGGAGGCCGAGGCGGTG
  TTCCGGGCCATCACCATTGCGGGCCGGATCAACTGCCCTGTGTACATCACCAAGGTCATGAGCAAGAGTG
  CAGCCGACATCATCGCTCTGGCCAGGAAGAAAGGGCCCCTAGTTTTTGGAGAGCCCATTGCCGCCAGCCT
  GGGGACCGATGGCACCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCGGCGTTCGTGACTTCCCCTCCC
  CTGAGCCCGGACCCTACCACGCCCGACTACTTGACCTCCCTACTGGCCTGTGGGGACTTGCAGGTCACAG
  GCAGCGGCCACTGTCCCTACAGCACTGCCCAGAAGGCGGTGGGCAAGGACAACTTTACCCTGATCCCCGA
  GGGTGTCAACGGGATAGAGGAGCGGATGACGGTCGTCTGGGACAAGGCGGTGGCTACTGGCAAAATGGAT
  GAGAACCAGTTTGTCGCTGTCACCAGCACCAATGCAGCCAAGATCTTTAACCTGTACCCAAGGAAAGGGC
  GGATTGCCGTGGGCTCGGATGCCGACGTGGTCATCTGGGACCCCGACAAGTTGAAGACCATAACAGCCAA
  AAGTCACAAGTCGGCGGTGGAGTACAACATCTTCGAGGGTATGGAGTGCCACGGCTCCCCACTAGTGGTC
  ATCAGCCAGGGCAAGATCGTCTTTGAAGACGGAAACATCAACGTCAACAAGGGCATGGGCCGCTTCATTC
  CGCGGAAGGCGTTCCCGGAGCACCTGTACCAGCGCGTCAAAATCAGGAATAAGGTTTTTGGATTGCAAGG
  GGTTTCCAGGGGCATGTATGACGGTCCTGTGTACGAGGTACCAGCTACACCCAAATATGCAACTCCCGCT
  CCTTCAGCCAAATCTTCGCCTTCTAAACACCAGCCCCCACCCATCAGAAACCTCCACCAGTCCAACTTCA
  GCTTATCAGGTGCCCAGATAGATGACAACAATCCCAGGCGCACCGGCCACCGCATCGTGGCGCCCCCTGG
  TGGCCGCTCCAACATCACCAGCCTCGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212919 representing NM_001014809
  Red=Cloning site Green=Tags(s)
  
  MADRRRAWNTEDDLPVYLARPGSAAQTPRQKYGGMFAAVEGAYENKTIDFDAYSVGRRGSARTPRSAGRP
  DAVGLPGPGGSEDTASDVSEPSGSAVSSPGERDERPPTLRIRRPAPRDLPLGRDNGQSDRLLIKGGRIIN
  DDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTYLQKPSQGMTAADDFFQGTRAA
  LVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDITSWYDGVREELEVLVQDKGVNSFQV
  YMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMGITGPEGHALSRPEELEAEAV
  FRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAASLGTDGTHYWSKNWAKAAAFVTSPP
  LSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIPEGVNGIEERMTVVWDKAVATGKMD
  ENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSHKSAVEYNIFEGMECHGSPLVV
  ISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSRGMYDGPVYEVPATPKYATPA
  PSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPPGGRSNITSLG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014809
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014809.1, NP_001014809.1
• RefSeq Size: 3155 bp
• RefSeq ORF: 2061 bp
• Locus ID: 1400
• Cytogenetics: 4p16.2
• Gene Summary: 'This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]'