ATP2B1 (NM_001001323) Human Tagged ORF Clone

CAT#: RG213177

ATP2B1 (GFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 1. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP2B1
• Synonyms: PMCA1; PMCA1kb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213177 ORF sequence, codon optimized.
  Due to the complexity of NM_001001323, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACATGGCAAACAACTCAGTTGCTTACAGTGGTGTGAAAAACTCTTTGAAGGAAGCTAATCATG
  ATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGCTCTCATGGAGCTCAGGTCCACAGATGCATTACG
  AAAAATACAGGAAAGCTATGGAGATGTCTATGGAATTTGCACCAAATTGAAAACATCTCCCAATGAAGGT
  TTAAGTGGAAACCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCTAAAA
  AGCCAAAAACCTTTCTTCAATTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATATTAGAAATTGC
  AGCCATAGTATCATTGGGCCTTTCTTTTTATCAGCCTCCAGAAGGGGATAATGCACTTTGTGGAGAAGTT
  TCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTGGATTGAAGGAGCTGCAATCCTCTTGTCTGTAG
  TGTGTGTGGTGTTAGTAACAGCTTTCAATGACTGGAGTAAGGAAAAACAGTTTAGAGGTTTGCAGAGCCG
  AATTGAACAAGAACAGAAGTTCACTGTCATCAGGGGTGGTCAGGTCATTCAGATACCTGTAGCTGACATT
  ACTGTTGGAGATATTGCTCAAGTGAAATATGGTGATCTTCTTCCAGCTGACGGCATACTTATTCAAGGCA
  ACGATCTTAAAATTGATGAAAGCTCATTGACTGGTGAATCAGATCATGTTAAAAAGTCTTTAGATAAGGA
  TCCCTTACTTCTATCAGGTACTCATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTAGGTGTA
  AATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGCTGGAGGTGAAGAGGAAGAGAAGAAAGATGAGA
  AGAAAAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAGAATCGCAACAAAGCAAAAGCCCAGGA
  TGGTGCAGCCATGGAAATGCAGCCATTGAAGAGTGAAGAAGGTGGAGATGGTGATGAAAAAGATAAAAAG
  AAAGCAAATTTGCCAAAAAAGGAAAAATCTGTTTTACAAGGGAAACTTACAAAACTGGCTGTTCAGATTG
  GCAAAGCAGGTCTGTTGATGTCTGCCATCACAGTTATCATTCTAGTATTATATTTTGTCATTGACACCTT
  CTGGGTTCAGAAAAGACCATGGCTTGCTGAGTGCACACCAATTTATATACAATACTTTGTGAAGTTCTTC
  ATTATTGGAGTTACAGTTTTAGTGGTCGCAGTGCCAGAAGGTCTTCCACTTGCAGTCACGATCTCACTGG
  CTTATTCAGTCAAAAAAATGATGAAAGATAATAACTTAGTAAGGCATCTGGATGCTTGTGAAACCATGGG
  AAATGCTACAGCTATTTGTTCAGATAAAACAGGAACTTTGACAATGAACAGAATGACAGTCGTTCAAGCT
  TACATAAATGAAAAACATTATAAAAAGGTTCCTGAACCAGAAGCTATTCCACCAAATATTTTGTCCTATC
  TTGTAACAGGAATTTCTGTGAATTGTGCTTATACATCAAAAATATTGCCACCAGAGAAAGAGGGTGGATT
  ACCTCGTCACGTTGGTAATAAAACTGAATGTGCCTTGTTGGGACTTCTTTTGGATTTAAAACGGGATTAT
  CAGGATGTTAGAAATGAAATACCAGAAGAAGCACTGTACAAAGTCTACACCTTCAATTCTGTTAGGAAGT
  CCATGAGTACTGTCCTGAAAAATTCAGATGGAAGTTATCGAATATTCAGCAAGGGTGCATCTGAGATAAT
  TCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAATGGTGAGGCAAAAGTATTCAGACCAAGGGACCGTGAT
  GATATTGTAAAAACTGTGATTGAACCGATGGCATCAGAAGGCTTGAGAACCATATGTCTTGCATTCAGAG
  ATTTTCCAGCAGGAGAACCAGAACCAGAGTGGGATAATGAAAATGATATTGTCACCGGCCTTACATGCAT
  TGCTGTTGTGGGGATTGAAGATCCTGTGAGACCTGAGGTGCCAGATGCAATTAAAAAGTGTCAGAGGGCT
  GGAATTACTGTGCGGATGGTCACTGGTGATAATATTAATACTGCTCGGGCCATTGCTACCAAATGTGGTA
  TTTTACATCCTGGGGAAGATTTTCTGTGCCTAGAAGGTAAAGATTTTAACAGAAGAATACGAAATGAAAA
  AGGAGAGATTGAGCAAGAGAGGATAGACAAGATTTGGCCAAAACTTCGAGTACTTGCAAGATCATCTCCT
  ACTGATAAGCATACACTGGTTAAAGGTATAATTGACAGCACTGTCTCAGACCAACGCCAGGTTGTAGCTG
  TAACTGGTGATGGTACAAATGATGGCCCAGCACTAAAGAAAGCAGATGTTGGATTTGCAATGGGTATTGC
  TGGAACTGATGTAGCTAAAGAAGCATCCGATATTATTCTCACAGATGACAACTTTACAAGCATTGTTAAA
  GCAGTTATGTGGGGACGAAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTCCAACTTACTGTTAATG
  TAGTAGCAGTGATTGTTGCTTTTACGGGCGCCTGCATTACTCAAGACTCACCGCTTAAGGCTGTGCAGAT
  GCTGTGGGTAAACCTCATAATGGATACACTCGCTTCCCTGGCTCTGGCAACGGAACCACCCACTGAGTCT
  CTCTTGCTTCGGAAACCTTATGGTAGAAATAAGCCTCTCATCTCACGTACAATGATGAAGAATATTTTGG
  GTCATGCATTCTATCAACTTGTAGTAGTCTTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGACATTGA
  TAGTGGAAGAAATGCTCCTTTGCATGCTCCTCCTTCAGAACATTATACTATTGTTTTTAATACCTTTGTG
  CTGATGCAACTTTTCAACGAAATAAATGCCCGGAAAATTCATGGTGAAAGAAATGTATTCGAAGGAATCT
  TTAACAATGCCATCTTCTGCACAATTGTTTTAGGCACTTTTGTGGTACAGATAATAATTGTGCAGTTTGG
  TGGAAAACCTTTCAGTTGTTCAGAACTTTCAATAGAACAGTGGCTATGGTCAATATTCCTAGGAATGGGA
  ACATTACTCTGGGGCCAGCTTATTTCAACAATTCCAACTAGCCGTTTAAAATTCCTCAAAGAAGCTGGTC
  ATGGAACACAAAAGGAAGAAATACCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGATCACGCTGA
  AAGGGAGTTGCGGCGTGGCCAAATCTTGTGGTTTAGAGGTCTGAACAGAATCCAAACACAGATGGATGTA
  GTGAATGCTTTCCAGAGTGGAAGTTCCATTCAGGGGGCTCTAAGGCGGCAACCCTCCATCGCCAGCCAGC
  ATCATGATGTAACAAATATTTCTACCCCTACACATGTAGTGTTTTCCTCTTCTACTGCTTCTACTACTGT
  GGGGTATTCGAGTGGTGAATGCATTTCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213177 representing NM_001001323
  Red=Cloning site Green=Tags(s)
  
  MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEG
  LSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEV
  SVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADI
  TVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGV
  NSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKK
  KANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFF
  IIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQA
  YINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGLLLDLKRDY
  QDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEAKVFRPRDRD
  DIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGIEDPVRPEVPDAIKKCQRA
  GITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSP
  TDKHTLVKGIIDSTVSDQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVK
  AVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTES
  LLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFV
  LMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMG
  TLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQMDV
  VNAFQSGSSIQGALRRQPSIASQHHDVTNISTPTHVVFSSSTASTTVGYSSGECIS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001323
• ORF Size: 3528 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001001323.1, NP_001001323.1
• RefSeq Size: 7121
• RefSeq ORF: 3531
• Locus ID: 490
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]