Eph receptor A5 (EPHA5) (NM_004439) Human Tagged ORF Clone

CAT#: RG213206

EPHA5 (GFP-tagged) - Human EPH receptor A5 (EPHA5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EPHA5
• Synonyms: CEK7; EHK-1; EHK1; EK7; HEK7; TYRO4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213206 representing NM_004439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGCTCGGGGCCCCGGGGTGCGGGACACCGGCGGCCCCCAAGCGGCGGCGGCGACACCCCCATCA
  CCCCAGCGTCCCTGGCCGGCTGCTACTCTGCACCTCGACGGGCTCCCCTCTGGACGTGCCTTCTCCTGTG
  CGCCGCACTCCGGACCCTCCTGGCCAGCCCCAGCAACGAAGTGAATTTATTGGATTCACGCACTGTCATG
  GGGGACCTGGGATGGATTGCTTTTCCAAAAAATGGGTGGGAAGAGATTGGTGAAGTGGATGAAAATTATG
  CCCCTATCCACACATACCAAGTATGCAAAGTGATGGAACAGAATCAGAATAACTGGCTTTTGACCAGTTG
  GATCTCCAATGAAGGTGCTTCCAGAATCTTCATAGAACTCAAATTTACCCTGCGGGACTGCAACAGCCTT
  CCTGGAGGACTGGGGACCTGTAAGGAAACCTTTAATATGTATTACTTTGAGTCAGATGATCAGAATGGGA
  GAAACATCAAGGAAAACCAATACATCAAAATTGATACCATTGCTGCCGATGAAAGCTTTACAGAACTTGA
  TCTTGGTGACCGTGTTATGAAACTGAATACAGAGGTCAGAGATGTAGGACCTCTAAGCAAAAAGGGATTT
  TATCTTGCTTTTCAAGATGTTGGTGCTTGCATTGCTCTGGTTTCTGTGCGTGTATACTATAAAAAATGCC
  CTTCTGTGGTACGACACTTGGCTGTCTTCCCTGACACCATCACTGGAGCTGATTCTTCCCAATTGCTCGA
  AGTGTCAGGCTCCTGTGTCAACCATTCTGTGACCGATGAACCTCCCAAAATGCACTGCAGCGCCGAAGGG
  GAGTGGCTGGTGCCCATCGGGAAATGCATGTGCAAGGCAGGATATGAAGAGAAAAATGGCACCTGTCAAG
  TGTGCAGACCTGGGTTCTTCAAAGCCTCACCTCACATCCAGAGCTGCGGCAAATGTCCACCTCACAGTTA
  TACCCATGAGGAAGCTTCAACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGAGAGTCTGATCCACCC
  ACAATGGCATGCACAAGACCCCCCTCTGCTCCTCGGAATGCCATCTCAAATGTTAATGAAACTAGTGTCT
  TTCTGGAATGGATTCCGCCTGCTGACACTGGTGGAAGGAAAGACGTGTCATATTATATTGCATGCAAGAA
  GTGCAACTCCCATGCAGGTGTGTGTGAGGAGTGTGGCGGTCATGTCAGGTACCTTCCCCGGCAAAGCGGC
  CTGAAAAACACCTCTGTCATGATGGTGGATCTACTCGCTCACACAAACTATACCTTTGAGATTGAGGCAG
  TGAATGGAGTGTCCGACTTGAGCCCAGGAGCCCGGCAGTATGTGTCTGTAAATGTAACCACAAATCAAGC
  AGCTCCATCTCCAGTCACCAATGTGAAAAAAGGGAAAATTGCAAAAAACAGCATCTCTTTGTCTTGGCAA
  GAACCAGATCGTCCCAATGGAATCATCCTAGAGTATGAAATCAAGTATTTTGAAAAGGACCAAGAGACCA
  GCTACACGATTATCAAATCTAAAGAGACAACTATTACTGCAGAGGGCTTGAAACCAGCTTCAGTTTATGT
  CTTCCAAATTCGAGCACGTACAGCAGCAGGCTATGGTGTCTTCAGTCGAAGATTTGAGTTTGAAACCACC
  CCAGTGTTTGCAGCATCCAGCGATCAAAGCCAGATTCCTGTAATTGCTGTGTCTGTGACAGTGGGAGTCA
  TTTTGTTGGCAGTGGTTATCGGCGTCCTCCTCAGTGGAAGTTGCTGCGAATGTGGCTGTGGGAGGGCTTC
  TTCCCTGTGCGCTGTTGCCCATCCAAGCCTAATATGGCGGTGTGGCTACAGCAAAGCAAAACAAGATCCA
  GAAGAGGAAAAGATGCATTTTCATAATGGGCACATTAAACTGCCAGGAGTAAGAACTTACATTGATCCAC
  ATACCTATGAGGATCCCAATCAAGCTGTCCACGAATTTGCTAAGGAGATAGAAGCATCATGTATCACCAT
  TGAGAGAGTTATTGGAGCAGGTGAATTTGGTGAAGTTTGTAGTGGACGTTTGAAACTACCAGGAAAAAGA
  GAATTACCTGTGGCTATCAAAACCCTTAAAGTAGGCTATACTGAAAAGCAACGCAGAGATTTCCTAGGTG
  AAGCAAGTATCATGGGACAGTTTGATCATCCTAACATCATCCATTTAGAAGGTGTGGTGACCAAAAGTAA
  ACCAGTGATGATCGTGACAGAGTATATGGAGAATGGCTCTTTAGATACATTTTTGAAGAAAAACGATGGG
  CAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGTATCTCTGCAGGAATGAAGTACCTTTCTGACA
  TGGGCTATGTGCATAGAGATCTTGCTGCCAGAAACATCTTAATCAACAGTAACCTTGTGTGCAAAGTGTC
  TGACTTTGGACTTTCCCGGGTACTGGAAGATGATCCCGAGGCAGCCTACACCACAAGGGGAGGAAAAATT
  CCAATCAGATGGACTGCCCCAGAAGCAATAGCTTTCCGAAAGTTTACTTCTGCCAGTGATGTCTGGAGTT
  ATGGAATAGTAATGTGGGAAGTTGTGTCTTATGGAGAGAGACCCTACTGGGAGATGACCAATCAAGATGT
  GATTAAAGCGGTAGAGGAAGGCTATCGTCTGCCAAGCCCCATGGATTGTCCTGCTGCTCTCTATCAGTTA
  ATGCTGGATTGCTGGCAGAAAGAGCGAAATAGCAGGCCCAAGTTTGATGAAATAGTCAACATGTTGGACA
  AGCTGATACGTAACCCAAGTAGTCTGAAGACGCTGGTTAATGCATCCTGCAGAGTATCTAATTTATTGGC
  AGAACATAGCCCACTAGGATCTGGGGCCTACAGATCAGTAGGTGAATGGCTAGAGGCAATCAAGATGGGC
  CGGTATACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGTGGCTCAGGTGACCTTGGAGG
  ATTTGAGACGGCTTGGAGTGACTCTTGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGAAATGAA
  GGTGCAGCTGGTAAACGGAATGGTGCCATTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213206 representing NM_004439
  Red=Cloning site Green=Tags(s)
  
  MRGSGPRGAGHRRPPSGGGDTPITPASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVM
  GDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSL
  PGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGF
  YLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEG
  EWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPP
  TMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSG
  LKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQ
  EPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETT
  PVFAASSDQSQIPVIAVSVTVGVILLAVVIGVLLSGSCCECGCGRASSLCAVAHPSLIWRCGYSKAKQDP
  EEEKMHFHNGHIKLPGVRTYIDPHTYEDPNQAVHEFAKEIEASCITIERVIGAGEFGEVCSGRLKLPGKR
  ELPVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIHLEGVVTKSKPVMIVTEYMENGSLDTFLKKNDG
  QFTVIQLVGMLRGISAGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKI
  PIRWTAPEAIAFRKFTSASDVWSYGIVMWEVVSYGERPYWEMTNQDVIKAVEEGYRLPSPMDCPAALYQL
  MLDCWQKERNSRPKFDEIVNMLDKLIRNPSSLKTLVNASCRVSNLLAEHSPLGSGAYRSVGEWLEAIKMG
  RYTEIFMENGYSSMDAVAQVTLEDLRRLGVTLVGHQKKIMNSLQEMKVQLVNGMVPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004439
• ORF Size: 3111 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004439.4, NP_004430.3
• RefSeq Size: 3349 bp
• RefSeq ORF: 3114 bp
• Locus ID: 2044
• Cytogenetics: 4q13.1-q13.2
• Domains: pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: 'This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]'