DIAPH1 (NM_001079812) Human Tagged ORF Clone

CAT#: RG213517

DIAPH1 (GFP-tagged) - Human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DIAPH1
• Synonyms: DFNA1; DIA1; DRF1; hDIA1; LFHL1; SCBMS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213517 representing NM_001079812
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCCCGGCGGGAGCCTGGGGCCCGGCCGCGGGACCCGGGACAAGAAGAAGGGCCGGAGCCCAG
  ATGAGCTGCCCTCGGCGGGCGGCGACGGCGGCAAATCTAAGAAATTTCTGGAGAGATTTACCAGCATGAG
  AATTAAGAAGGAGAAGGAAAAGCCCAATTCTGCTCATAGAAATTCTTCTGCATCATATGGGGATGATCCC
  ACAGCACAGTCATTGCAAGATGTTTCAGATGAACAAGTGCTGGTTCTCTTTGAACAGATGCTGCTGGATA
  TGAACCTGAATGAGGAGAAACAGCAACCTTTGAGGGAGAAGGACATCATCATCAAGAGGGAGATGGTGTC
  CCAATACTTGTACACCTCCAAGGCTGGCATGAGCCAGAAGGAGAGCTCTAAGTCTGCCATGATGTATATT
  CAGGAGTTGAGGTCAGGCTTGCGGGATATGCCTCTGCTCAGCTGCCTGGAGTCCCTTCGTGTGTCTCTCA
  ACAACAACCCTGTCAGTTGGGTGCAAACATTTGGTGCTGAAGGCTTGGCCTCCTTATTGGACATTCTTAA
  ACGACTTCATGATGAGAAAGAAGAGACTGCTGGGAGTTACGATAGCCGGAACAAGCATGAGATCATTCGC
  TGCTTGAAAGCTTTTATGAACAACAAGTTTGGAATCAAGACCATGTTGGAGACAGAAGAAGGAATCCTAC
  TGCTGGTCAGAGCCATGGATCCTGCTGTTCCCAACATGATGATTGATGCAGCTAAGCTGCTTTCTGCTCT
  TTGTATTCTACCGCAGCCAGAGGACATGAATGAAAGGGTTTTGGAGGCAATGACAGAAAGAGCTGAGATG
  GATGAAGTGGAACGTTTCCAGCCGCTGCTGGATGGATTAAAAAGTGGAACCACTATTGCACTGAAGGTTG
  GATGCCTACAGCTGATCAATGCTCTCATCACACCAGCGGAGGAACTTGACTTCCGAGTTCACATCAGAAG
  TGAACTGATGCGTTTGGGGCTACATCAGGTGTTGCAGGACCTTCGAGAGATTGAAAATGAAGATATGAGA
  GTGCAACTAAATGTGTTTGATGAACAAGGGGAAGAGGATTCCTATGACCTGAAGGGACGGCTGGATGACA
  TTCGCATGGAGATGGATGACTTTAATGAAGTCTTTCAGATTCTCTTAAACACAGTGAAGGATTCAAAGGC
  AGAGCCACACTTCCTTTCCATCCTGCAGCACTTACTCTTGGTCCGAAATGACTATGAGGCCAGACCTCAG
  TACTATAAGTTGATTGAAGAATGTATTTCCCAGATAGTTCTGCACAAGAACGGGGCTGATCCTGACTTCA
  AGTGCCGGCACCTCCAGATTGAGATTGAGGGATTAATTGATCAAATGATTGATAAGACAAAGGTGGAGAA
  ATCTGAAGCCAAAGCTGCAGAGCTGGAAAAGAAGTTGGACTCAGAGTTAACAGCCCGACATGAGCTACAG
  GTGGAAATGAAAAAGATGGAAAGTGACTTTGAGCAGAAGCTTCAAGATCTTCAGGGAGAAAAAGATGCAC
  TGCATTCTGAAAAGCAGCAAATTGCCACAGAGAAACAGGACCTGGAAGCAGAGGTGTCCCAGCTCACAGG
  AGAGGTTGCCAAGCTGACAAAGGAACTGGAAGATGCCAAGAAAGAAATGGCTTCCCTCTCTGCGGCAGCT
  ATTACTGTACCTCCTTCTGTTCCTAGTCGTGCTCCTGTTCCCCCTGCCCCTCCTTTACCTGGTGACTCTG
  GCACTATTATTCCACCACCACCTGCTCCTGGGGATAGTACCACTCCTCCTCCTCCTCCTCCTCCTCCTCC
  TCCACCTCCTTTGCCTGGGGGTGTTTGCATCTCCTCACCCCCTTCTTTACCTGGAGGTACTGCTATCTCT
  CCACCCCCTCCTTTGTCTGGGGATGCTACCATCCCTCCACCCCCTCCTTTGCCTGAGGGTGTTGGCATCC
  CTTCACCCTCTTCTTTGCCTGGAGGTACTGCCATCCCCCCACCTCCTCCTTTGCCTGGGAGTGCTAGAAT
  CCCCCCACCACCACCTCCTTTGCCTGGGAGTGCTGGAATTCCCCCCCCACCTCCTCCCTTGCCTGGAGAA
  GCAGGAATGCCACCTCCTCCTCCCCCTCTTCCTGGTGGTCCTGGAATCCCTCCACCTCCTCCATTTCCCG
  GAGGCCCTGGCATTCCTCCACCTCCACCCGGAATGGGTATGCCTCCACCTCCCCCATTTGGATTTGGAGT
  TCCTGCAGCCCCAGTTCTGCCATTTGGATTAACCCCCAAAAAGCTTTATAAGCCAGAGGTGCAGCTCCGG
  AGGCCAAACTGGTCCAAGCTTGTGGCTGAGGACCTCTCCCAGGACTGCTTCTGGACAAAGGTGAAGGAGG
  ACCGCTTTGAGAACAATGAACTTTTCGCCAAACTTACCCTTACCTTCTCTGCCCAGACCAAGACTTCCAA
  AGCCAAGAAGGATCAAGAAGGTGGAGAAGAAAAGAAATCTGTGCAAAAGAAAAAAGTAAAAGAGTTAAAG
  GTGTTGGATTCAAAGACAGCCCAGAATCTCTCAATCTTTTTGGGTTCCTTCCGCATGCCCTATCAAGAGA
  TTAAGAATGTCATCCTGGAGGTGAATGAGGCTGTTCTGACTGAGTCTATGATCCAGAACCTCATTAAGCA
  AATGCCAGAGCCAGAGCAGTTAAAAATGCTTTCTGAACTGAAGGATGAATATGATGACCTGGCTGAGTCA
  GAGCAGTTTGGCGTGGTGATGGGCACTGTGCCCCGACTGCGGCCTCGCCTCAATGCCATTCTCTTCAAGC
  TACAATTCAGCGAGCAAGTGGAGAATATCAAGCCAGAGATTGTGTCTGTCACTGCTGCATGTGAGGAGTT
  ACGTAAGAGTGAGAGCTTTTCCAATCTCCTAGAGATTACCTTGCTTGTTGGAAATTACATGAATGCTGGC
  TCCAGAAATGCTGGTGCTTTTGGCTTCAATATCAGCTTCCTCTGTAAGCTTCGAGACACCAAGTCCACAG
  ATCAGAAGATGACGTTGTTACACTTCTTGGCTGAGTTGTGTGAGAATGACTATCCCGATGTCCTCAAGTT
  TCCAGACGAGCTTGCCCATGTGGAGAAAGCCAGCCGAGTTTCTGCTGAAAACTTGCAAAAGAACCTAGAT
  CAGATGAAGAAACAAATTTCTGATGTGGAACGTGATGTTCAGAATTTCCCAGCTGCCACAGATGAAAAAG
  ACAAGTTTGTTGAAAAAATGACCAGCTTTGTGAAGGATGCACAGGAACAGTATAACAAGCTGCGGATGAT
  GCATTCTAACATGGAGACCCTCTATAAGGAGCTGGGCGAGTACTTCCTCTTTGACCCCAAGAAGTTGTCT
  GTTGAAGAATTTTTCATGGATCTTCACAATTTTCGGAATATGTTTTTGCAAGCAGTCAAGGAGAACCAGA
  AGCGGCGGGAGACAGAAGAAAAGATGAGGCGAGCAAAACTAGCCAAGGAGAAGGCAGAGAAGGAGCGGCT
  AGAGAAGCAGCAGAAGAGAGAGCAACTCATAGACATGAATGCAGAGGGCGATGAGACAGGTGTGATGGAC
  AGTCTTCTAGAAGCCCTGCAGTCAGGGGCAGCATTCCGACGGAAGAGAGGGCCCCGTCAAGCCAACAGGA
  AGGCCGGGTGTGCAGTCACATCTCTGCTAGCTTCGGAGCTGACCAAGGATGATGCCATGGCTGCTGTTCC
  TGCCAAGGTGTCCAAGAACAGTGAGACATTCCCCACAATCCTTGAGGAAGCCAAGGAGTTGGTTGGCCGT
  GCAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213517 representing NM_001079812
  Red=Cloning site Green=Tags(s)
  
  MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDP
  TAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYI
  QELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIR
  CLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEM
  DEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMR
  VQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQ
  YYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQ
  VEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAA
  ITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPLPGGVCISSPPSLPGGTAIS
  PPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGE
  AGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLR
  RPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELK
  VLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAES
  EQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAG
  SRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLD
  QMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLS
  VEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMD
  SLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGR
  AS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079812
• ORF Size: 3786 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079812.1, NP_001073280.1
• RefSeq Size: 5718 bp
• RefSeq ORF: 3792 bp
• Locus ID: 1729
• Cytogenetics: 5q31.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton
• Gene Summary: 'This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'